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Diss Factsheets
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EC number: 200-839-4 | CAS number: 75-10-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The 96-h EC50 for algae estimated using ECOSAR v1.11 for neutral organics is 313 mg/l.
Given the physical nature of difluoromethane and due to the difficulty to test it meaningfully, no experimental aquatic results are available. Instead, QSAR methods were used to estimate the toxicity of the substance to aquatic organisms. The effect concentrations of difluoromethane were estimated using the method described in the EU Technical Guidance Document (2003) and/or the US EPA program ECOSAR (v1.11, 2012). The measured log Kow value of 0.21 was used for the predictions using both QSARs.
Only calculated effect concentrations are available, all calculations are reliable and the substance properties fall within the applicability domain of the QSARs as described in the QPRF report attached. The calculated acute effect parameter using ECOSAR v1.11 resulted in an EC50 (96h) = 313 mg/l and this result is used in the assessment.
In a supporting read-across study with the analogue substance HFC-245fa, slight growth inhibition was observed at 118 mg/l test substance. The 72 hour EC50 was greater than 118 mg/l. In a second supporting read-across study with the analogue substance HFC-365mfc, no EC50 for biomass and growth rate could be determined at any timepoint (72h-EC50 > 114 mg/l). A 72h-NOEC of 13.2 mg/l was determined both for biomass and growth rate. Both studies support the fact that substances in this category are not likely to be toxic to algae, even when volatilization is minimized.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 313 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.