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EC number: 216-319-5 | CAS number: 1558-33-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dichloro(chloromethyl)methylsilane
- EC Number:
- 216-319-5
- EC Name:
- Dichloro(chloromethyl)methylsilane
- Cas Number:
- 1558-33-4
- Molecular formula:
- C2H5Cl3Si
- IUPAC Name:
- dichloro(chloromethyl)methylsilane
- Details on test material:
- Designation Chlormethyl-methyl-dichlorsilan
CAS No. 1558-33-4
Date of receipt March 6th, 2002
Aggregate state under storage conditions liquid at + 20°C
Storage conditions at room temperature, dark, in original container tightly closed
Colour colourless
Solvent abs. ethylene glycol dimethylether
Stability in solvent at +4°C in the dark: > 1 day
Expiry date March 2003
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 102
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 10, 31.6, 100, 316 and 1000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethylene glycol dimethylether
- Justification for choice of solvent/vehicle: solubility
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- strains TA 1535, TA 100 without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- strain TA 98 without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- strain TA 1537 without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- strain TA 102 without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-anthracene amide
- Remarks:
- strains TA 98, TA 102, TA 1537 with metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- strains TA 100, TA 1535 with metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) for 1st experiment, preincubation for second experiment
DURATION
- Preincubation period: 60 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: - Evaluation criteria:
- The statisIn the test laboratory, a test chemical is considered to show a positive response if the number of revertants is significantly increased (p <= 0.05, U-test according to MANN and WHITNEY, compared with the solvent control to at least 2-fold of the solvent control for TA 98, TA 100 and TA 102 and 3-fold of the solvent control for TA 1535 and TA 1537 in both independent experiments; in addition, a significant (p <= 0.05) concentration (log value)-related effect 5pearman's rank correlation coefficient, is observed; positive results have to be reproducible and the histidine independence of the revertants has to be confirmed by streaking random samples on histidine-free agar plates.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No mutagenic effect (no increase in revertant colony numbers as compared with control counts) was observed for Chlormethyl-methyl-dichlorsilan tested up to a concentration of 1000 Jµg/plate in any of the 5 test strains in two independent experiments without and with metabolic activation (plate incorporation or preincubation test). - Executive summary:
Chlormethyl-methyl-dichlorsilan was examined in the 5 Sa/monella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 in two independent experiments, each carried out without and with metabolic activation (a microsomal preparation derived from Aroclor 1254-induced rat liver). The first experiment was carried out as a plate incorporation test and the second as a preincubation test.
Preliminary test
Chlormethyl-methyl-dichlorsilan was examined in a preliminary cytotoxicity test without metabolic activation in test strain TA 100. Ten concentrations of Chlormethylmethyl- dichlorsilan ranging from 0.316 to 5000 µg/plate were examined in the preliminary test using strain TA 100. Cytotoxicity (scarce background lawn) was noted at the concentrations of 1000 up to 5000 µg/plate. Hence, 1000 µg/plate was chosen as the top concentration for the main study.
Main study
Five (5) concentrations ranging from 10 to 1000 µg Chlormethyl-methyl-dichlorsilan/ plate were employed in two independent experiments each carried out without and with metabolic activation.
Cytotoxicity
In the plate incorporation test without and with metabolic activation cytotoxicity (scarce background lawn) was noted at the top concentration of 1000 µg Chlormethyl-methyldichlorsilan/ plate in all test strains. In the preincubation test without and with metabolic activation cytotoxicity (scarce background lawn) was noted at the concentration of 1000 µg Chlormethyl-methyldichlorsilan/ plate in all test strains. Pronounced cytotoxicity (reduction of the number of revertants by more than 50%) was noted at the concentration of 1000 µg/plate in test strains TA 1535 and TA 1537.
Mutagenicity
No mutagenic effect (no increase in revertant colony numbers as compared with control counts) was observed for Chlormethyl-methyl-dichlorsilan tested up to 1000 µg Chlormethyl-methyl-dichlorsilan/plate in any of the 5 test strains in two independent experiments without and with metabolic activation (plate incorporation or preincubation test).
In conclusion, under the present test conditions Chlormethyl-methyl-dichlorsilan tested up to cytotoxic concentrations caused no mutagenic effect in the Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test nor in the preincubation test each carried out without and with metabolic activation.
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