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EC number: 620-056-5 | CAS number: 874195-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Jun - 15 Jul 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF guideline (12 Nou(h)san No 8147 (2000))
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were analysed for the test medium of all treatment levels and the control on day 0 and 3.
- Vehicle:
- yes
- Details on test solutions:
- 1073 mg test substance ad 10 mL dimethylformamid was prepared immediately prior to test initiation. The stock solution was well agitated on a magnetic stirrer for 5 minutes before further use. An adequate amount of the stock solution was transferred to a dilution series to obtain the concentratio levels used in the study.
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 61.81
- Source (laboratory, culture collection): Collection of Algal Cultures, Inst. for Plant Physiology, University of Göttingen, Germany
- Age of inoculum (at test initiation): 4 days old, log phase, cell density 10000 cells/mL
- Method of cultivation: 200 μL of a 7-10 days old stock culture was transferred into a 250 mL cotton plugged Erlenmeyer flask containing 50 mL of nutrient medium (Bringmann and Kühn, 1980) once every week. Stock cultures of algae were kept at 22 ± 2°C with 24 h light.
CULTURE
- Culture conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21.3 - 22.4°C
- pH:
- 7.8 - 8.2
- Nominal and measured concentrations:
- Nominal: 0 (control), 0 (solvent control), 0.0300, 0.0960, 0.307, 0.980, 3.13 and 10.0 mg a.s./L
Recovery: Inital 96 % to 108 % of nominal (average 102 %). On day three 92 % to 100 % of nominal (average 95 %) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks with a total volume of 300 mL
- Material, fill volume: glass, 150 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, prepared according to the mentioned guideline
- Source/preparation of dilution water: deionized well water
- The stock solutions were sterilized by membrane filtration (mean pore diameter 0.2 μm) and stored at 4°C.
- The pH is adjusted to 8.3 ± 0.2 with NaOH
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Shaking: The shaker table rotated at approximately 100 oscillations per minute
- Photoperiod: continuous light
- Light intensity and quality: 4,440 – 8,880 lux; variation within the test: ± 15 % lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: with counting chamber after 0, 24, 48, and 72 h - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % CI: 5.29 – 7.51 mg a.s./L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.096 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- Test conditions met all validity criteria, given by the mentioned guideline.
No statistically significant different was determined between the growth rates of the control and the solvent control. - Results with reference substance (positive control):
- Positive control results are documented and archived together with strain protocols separatly.
Reference
Description of key information
Pseudokirchnerella subcapitata EC50 (72h) = 6.23 mg/L (nominal, based on growth)
Pseudokirchnerella subcapitata NOEC (72h) = 0.096 mg/L (nominal, based on growth)
The test item was initially suspended in solubilizer.
Key value for chemical safety assessment
Additional information
One study is available on the toxicity of triafamone (CAS No. 874195-61-6) to the unicellular freshwater green algae Pseudokirchnerella subcapitata (Bruns, 2010). The study was performed according to OECD guideline 201 and GLP, with analytical monitoring of the test item concentration. The measured recovery rates at the end of test were above 90 percent. Therefore it was proven that the test item was stable during the 72 hours test duration and the results can be given as nominal values. Since the substance has a moderate water solubility (approx. 33 mg/L) the test item was initially dissolved in dimethylformamide. No statistically significant different was determined between the growth rates in the controls and the solvent controls. In the treated samples the algae growth was inhibited, which results in an EC50 (72h) value of 6.23 mg/L triafamone. The no observed effect concentration (NOEC) after 72 hours exposure is reported with 0.096 mg/L triafamone.
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