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EC number: 700-908-3 | CAS number: 19444-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Instead of a centrifuged sludge a settled sludge was used.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
- EC Number:
- 700-908-3
- Cas Number:
- 19444-21-4
- Molecular formula:
- C7H12O3
- IUPAC Name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge collection from the sewage treatment plant of CH-4153 Reinach on November 3, 1999.
The pH after collection was 7.7.
The preparation was carried out according to the method described in the guideline.
The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 7.9.
The sludge concentration in the test bottles was 1.75 g suspended solids/L.
Study design
- Test type:
- other: aeration
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2°c
- pH:
- The pH after collection was 7.7.
The pH before use was 7.9. - Nominal and measured concentrations:
- Test item: 100.0, 32.0, 10.0, 3.2 and 1.0 mg/L
Reference item: 32.0, 10.0 and 3.2 mg/L - Details on test conditions:
Nutrient Solution:
16g Peptone
11g Meat extract
3.0g Urea
0.7g NaCL
0.4g CaCl2.2H2O
0.2g MgSO4.7H2O
2.8g K2HPO4
Were dissolved and the volume made up to one litre with de-chlorinated drinking water.
Vessels:
250 ml BOD flasks with gas inlet
Water:
De-chlorinated drinking water
Temperature:
20 ± 2 °C
Duration:
3 hours.
Stock solution:
200.8 mg test item were mixed and made up to 1000 ml with water.
The stock solution was freshly prepared at the start of the test.
Test concentrations:
Test item: 100.0, 32.0, 10.0, 3.2 and 1.0 mg/L
Reference item: 32.0, 10.0 and 3.2 mg/L
Test Concentrations:
Test item: 100.0, 32.0, 10.0, 3.2 and 1.0 mg/L
Reference item: 32.0, 10.0 and 3.2 mg/L
Calculated amounts of test item to produce the desired test concentrations were mixed with 114 ml water and 6.4 ml nutrient solution
(= test solution). Then 80 ml of the sludge were added. The test item, 6.4 ml of the nutrient solution, 114 ml water and 80 ml of the sludge were
mixed in 250 ml BOD flasks and aerated immediately.
Measurements:
Oxygen consumption per hour in mg/L with a ORION – Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and plotted on a recorder.- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
The inhibitory effect of th tet item was expressed as a percentage of the mean respiration rates of two controls. Following values were calculated based on nominal concentrations:
The test item was tested at nominal concentrations of 1.0, 3.2, 10.0, 32.0 and 100 mg/L after a contact time of 3 hours. The calculation of the following effect concentrations was based on these nominal values.
EC50 (3h) : >100 mg/L
EC20 (3h) : >100 mg/L
EC80 (3h) : >100 mg/L- Results with reference substance (positive control):
- For the reference item (tested nominal concentrations : 3.2, 10.0, 32.0 mg/L):
EC50 (3h) : >11.4 mg/L
EC20 (3h) : >4.1 mg/L
EC80 (3h) : >31.4 mg/L - Reported statistics and error estimates:
- No error estimates reported
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC 50 (3h) for CA 2215 A in this test alone, is >100 mg/L. Based on the inherenttoxicity of CA 2215 A in this test, the test item can be classified as “not toxic to bacteria“.
- Executive summary:
In the test with CA 2215 A the temperature was maintained constant at 20 ± 1°C.
The pH of sludge was 7.7 after collection and 7.9 after preparation (before use). The pH of the mixtures in the test bottles was 8.2 – 8.3 at the end of exposure time.
The EC50 (3h) of the reference item 3,5-Dichlorophenol was 11.4 mg/L.
The inhibitory effect of the test item was expressed as a percentage of the mean respiration rates of two controls. Following values were calculated based on nominal concentrations:
EC50 (3h): > 100 mg/L
EC20 (3h): > 100 mg/L
EC80 (3h): > 100 mg/L
The EC 50 (3h) for CA 2215 A in this test alone, the test item can be classified as “not toxic to bacteria“.
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