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EC number: 231-701-1 | CAS number: 7691-02-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1994-05-03 to 1994-05-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted according to an appropriate OECD test guideline, and in compliance with GLP, and was considered to be reliability 1 (reliable without restrictions).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.3
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations at the start and end of the test in fresh and/or old media as applicable
- Sampling method: Not reported
- Sample storage conditions before analysis: Not reported - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test media prepared by mixing for 18 h followed by filtration. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81.SAG
- Source (laboratory, culture collection): Institute for Water, Ground and Air Hygiene, Berlin (Germany)
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A pre-culture is produced from an original culture by super-inoculation three days before the test begins. From this culture the test cultures are inoculated at a density of about 20000 cells/ml. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- No data
- Test temperature:
- 24+/-2ºC
- pH:
- 8.5-9.3 at start of test
8.7-9.8 at end of test - Dissolved oxygen:
- No data
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Target test substance concentrations were 1.4, 3.1, 6.4, 14, 31, 64 and 140 mg/L.
The 72-hour values were 2.0, 2.7, 5.6, 11.9, 40.7, 66.4, and 151 mg/L.
The geometric mean of analytical values at 0 and 72 hours deviated by <20% from that of the nominal concentrations (except at the target concentration of 1.4 mg/L).
The nominal concentrations were used to present and interpret the test results. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open Erlenmeyer flasks
- Material: glass
- Aeration: none
- Initial cells density: 20000 cells/ml
- Control end cells density: 1130000 cells/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: start and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 8000 Lux
EFFECT PARAMETERS MEASURED: Algae cell counts were made photometrically at 0, 24, 48 and 72 hrs.
- Determination of cell concentrations: spectrophotometer (absorption at 685 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: test substance hydrolysis products
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Reported statistics and error estimates:
- The ECx values were determined by Probit analysis. The NOEC was taken as the EC10 values.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour EC50 value of 50 mg/L and a NOEC of 7.5 mg/L has been determined for the effects of the test substance on growth rate of Scenedesmus subspicatus. It is likely that the test organisms were exposed to the hydrolysis products of the substance.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2004-03-11 to 2004-03-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Negative Control, 31, 63, 125, 250, 500 and 1000 mg/L
- Sampling method: Test concentrations were measured in each treatment at test initiation and at test termination.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct addition of test article to freshwater algal medium
- Controls: Freshwater Algal Medium (OECD 201) - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Original algal cultures were obtained from the University of Toronto Culture Collection, and had been maintained in culture medium at Wildlife International, Ltd., Easton, Maryland. Algal cells used in this test were obtained from Wildlife International, Ltd. cultures that had been actively growing in culture medium for at least two weeks prior to test initiation. The culture was last transferred to fresh medium three days prior to test initiation.
- Culture medium: The algal cells were cultured and tested in freshwater algal medium. Stock nutrient solutions were prepared by adding reagent-grade chemicals to purified Wildlife International, Ltd. well water. The test medium then was prepared by adding appropriate volumes of the stock nutrient solutions to purified well water (NANOpure® water). The pH of the medium was adjusted to 7.9 using 10% HCl and the medium was sterilized by filtration (0.22 μm) prior to use.
- Initial density: 10,000 cells/mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Test temperature:
- 23.6 to 24.6 ºC
- pH:
- 7.9 at test initiation
8.3 to 9.9 at test termination - Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Negative Control, 31, 63, 125, 250, 500 and 1000 mg/L.
Measured concentrations:
Test concentrations declined to less than 70% of nominal at test termination. Consequently, the results of the study were based on Day 0 measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250-mL Erlenmeyer flasks plugged with cotton stoppers containing 100 mL of test solution.
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): static test
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater algal growth medium (OECD 201) with NANOpure water (pH: 7.9)
- Culture medium different from test medium: no
- Intervals of water quality measurement: Start and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: Continuous
- Light intensity and quality: 3900 to 4700 lux, continuous cool-white fluorescent lighting
EFFECT PARAMETERS MEASURED: Cell densities measured at start of test and then at 24 hour intervals. Cell densities were used to determine effects on average-specific growth rate and biomass.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 555 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 141-612
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 135 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 683 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 641-727
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 566 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 409-618
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 625 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 555-702
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 053 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not calculable
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 522 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 053 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not calculable
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 750 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not calculable
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 684 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Was control response satisfactory: Yes
Observations: There were no noticeable changes in cell morphology in any of the tested concentrations in comparison to the control.
- Reported statistics and error estimates:
- Statistical methods: Non-linear regression, linear interpolation and Dunnett's test
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour EC50 value of >1053 mg/L and NOEC of 70 mg/L have been determined for the effects of the test on growth rate of Selenastrum capricornutum (new name: Pseudokirchneriella subcapitata) based on initial measured concentrations of the test substance. When expressed relative to geometric mean measured concentrations the equivalent values are >684 mg/L and 44 mg/L.
Referenceopen allclose all
Table 1. Results of analysis of test media
Nominal concentration (mg/L) | Measured concentration at the start of the test (mg/L) | Measured concentration at end of test (mg/L) | Concentration used to assess results (mg/L) |
0 (Control) | - | - | - |
1.4 | 2.0 | 2.0 | 1.4 |
3.1 | 4.0 | 2.7 | 3.1 |
6.4 | 7.8 | 5.6 | 6.4 |
14 | 13.9 | 11.9 | 14 |
31 | 32.4 | 40.7 | 31 |
64 | 70.4 | 66.4 | 64 |
140 | 148 | 151 | 140 |
Table 2. Test results
Test concentration (mg/L) | Mean cell concentration at t0 (cells/ml) | Mean cell concentration at t24 (cells/ml) | Mean cell concentration at t48 (cells/ml) | Mean cell concentration at t72 (cells/ml) |
0 (Control) | 20000 | 120000 | 370000 | 1130000 |
1.4 | 20000 | 100000 | 360000 | 940000 |
3.1 | 20000 | 120000 | 320000 | 870000 |
6.4 | 20000 | 120000 | 340000 | 990000 |
14 | 20000 | 100000 | 300000 | 710000 |
31 | 20000 | 70000 | 220000 | 430000 |
64 | 20000 | 50000 | 70000 | 80000 |
140 | 20000 | 40000 | 40000 | 40000 |
Table 1. Test results (cell densities in cells/mL)
Day 0 Measured Concentration (mg/L)
0 33 70 135 257 522 1053
0-Hour 10000 10000 10000 10000 10000 10000 10000
24-Hour 72000 75000 67000 67000 60000 51000 22000
48-Hour 396000 389000 345000 322000 263000 193000 44000
72-Hour 1780000 1700000 1680000 1090000 882000 995000 172000
96-Hour 4660000 4620000 4690000 4740000 3810000 3500000 570000
Table 2. Percent biomass/growth rate inhibition per concentration
Day 0 Measured Concentration (mg/L)
33 70 135 257 522 1053
72-Hour Cell Density 4.6 5.8 39 51 44 90
96-Hour Cell Density 0.74 -0.70 -1.8 18 25 88
72-Hour Biomass 3.4 8.0 32 45 46 90
96-Hour Biomass 2.3 3.1 16 32 35 89
72-Hour Growth Rate 0.94 1.2 9.5 14 11 45
96-Hour Growth Rate 0.12 -0.11 -0.30 3.3 4.6 34
Description of key information
Algal 72 hr EC50 50 mg/l (nominal) (EU method C.3), read-across from a structurally related substance, 1,1,1,3,3,3-hexamethyldisilazane (CAS 999-97-3). The EC50 is equivalent to 28 mg/l when expressed in terms of the silanol hydrolysis product, trimethylsilanol and 5 mg/l in terms of NH3.
Algal 72 hr EC50 >684 mg/l and NOEC 44 mg/l (geometric mean measured) (OECD 201), read-across from an analogous substance, trimethylsilanol (CAS 1066-40-6).
Algal 72 hour EC50 >89 mg/l (nominal), read-across from an analogous, trimethoxy(vinyl)silane (CAS 2768-02-7). The EC50 is equivalent to >64 mg/l when expressed in terms of the silanol hydrolysis product, vinylsilanetriol.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 44 mg/L
Additional information
There are no reliable algal inhibition data for N-(dimethylvinylsilyl)-1,1-dimethyl-1-vinylsilylamine, therefore good quality data have been read-across for the effects of two read-across substances: 1,1,1,3,3,3-hexamethyldisilazane (CAS 999-97-3), and trimethylsilanol (CAS 1066-40-6) based on structural similarity and production of ammonia, and structural similarity and log Kow of the silanol hydrolysis product of the registered substance, respectively. In addition, reliable supporting data are presented for trimethoxy(vinyl)silane (CAS 2768-02-7) to demonstrate the low toxicity of the vinyl group.
The EC50 values of 50 (72 hr), >684 (72 hr) and >89 (72 hour) mg/l and NOECs of 7.5, 44 and ≥89 mg/l (nominal, measured and nominal respectively) have been determined for the effects of the read-across substances 1,1,1,3,3,3-hexamethyldisilazane (CAS 999-97-3), trimethylsilanol (CAS 1066-40-6) and trimethoxy(vinyl)silane (CAS 2768-02-7), respectively, on growth rate of Scenedesmus subspicatus (new name: Desmodesmus subspicatus) for the first two substances and on growth rate of Pseudokirchnerella subcapitata for trimethoxy(vinyl)silane. The trimethylsilanol (CAS 1066-40-6) study results are reported in terms of the geometric mean measured concentrations, due to the reduction in test substance recovery by the end of the test (<70%).
The data on trimethylsilanol (CAS 1066-40-6) are used to derive the PNEC for the silanol hydrolysis product, whereas the data for 1,1,1,3,3,3-hexamethyldisilazane (CAS 999-97-3) are used to establish the classification and labelling of the registered substance due to the presence of the ammonia hydrolysis product, which drives toxicity. The PNECs for ammonia are derived based on literature data.
For trimethylsilanol, a solution at 100 mg/l is predicted to contain >99.9% monomer. At loadings above about 500 - 1000 mg/l the concentration of the dimer is predicted to exceed its solubility, resulting in formation of a separate phase. During the algae test with trimethylsilanol, less than 20% growth inhibition was observed up to the 522 mg/l loading level and the EC50 is considered to be above the highest concentration tested. In addition, the dimer that could be formed due to the condensation of two trimethylsilanol molecules is hexamethyldisiloxane (HMDS, CAS 107-46-0). HMDS is classified as Aquatic Acute 1 H400 and Aquatic Chronic 2 H411[1] and therefore its presence may impact the toxicity results. However, the estimated concentration of HMDS is 0.04, 0.2, 0.3, 0.6 and 0.9 mg/l at 100, 200, 300, 400 and 500 mg/l of TMS respectively, and there was no reporting of undissolved test material or droplets being formed, which would otherwise clearly indicate the formation of HMDS. Furthermore, the log Kowof trimethylsilanol (1.62) is such that effects at relatively high loadings may be observed. Therefore, any condensation reactions that may have occurred did not significantly impact the results of the study and it is concluded that the study is considered to be reliable and sufficient for the assessment as part of the Weight of Evidence approach. This conclusion is supported by the analogous EC50 results obtained in the two algal studies with TMS.
In view of the exposure regime and the fast hydrolysis rates of 1,1,1,3,3,3-hexamethyldisilazanethyldisilazane (CAS 999-97-3) and trimethoxy(vinyl)silane (CAS 2768-02-7) it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substances, trimethylsilanol and ammonia, and vinylsilanetriol and methanol, respectively.
1,1,1,3,3,3-Hexamethyldisilazane (CAS 999-97-3)
The results may be expressed in terms of concentration of the hydrolysis product, trimethylsilanol, by applying a molecular weight correction:
(MW of silanol = 90.2 / MW of parent = 161.4) * EC50/NOEC mg/l = 28 mg/l EC50 and 4.2 mg/l NOEC
The results may also be expressed in terms of ammonia (NH3), by applying a molecular weight correction:
(MW of ammonia 17.03 / MW parent 161.4) * EC50/NOEC mg/l = 5.3 mg/l EC50 and 0.8 mg/l.
However, data with 1,1,1,3,3,3-hexamethyldisilazane are read-across for the purpose of classification and labelling and the relevant values are those determined in terms of the parent substance.
Trimethoxy(vinyl)silane (CAS 2768-02-7)
The results may be expressed in terms of concentration of the hydrolysis product, vinylsilanetriol, by applying a molecular weight correction:
(MW of silanol = 106.15 / MW of parent = 148.24) * EC50 or NOEC value thus 106.15/148.24 * EC50 of >89 mg/L and NOEC of ≥89 mg/L = EC50 >64 mg/L and NOEC ≥64 mg/L as vinylsilantriol.
These values are read across to dimethylvinylsilanol without further correction.
Refer to the discussion in the Section 6 endpoint summary (Section 7.0 of CSR) for further discussion of the approach to chemical safety assessment for this registration substance. The justification for read-across can be found in IUCLID Section 13.
Ammonia
Reliable short-term test results are available for ammonia sourced from the Environment Agency Proposed EQS for Water Framework Directive Annex VIII Substances: Ammonia (unionised) 2007. A five day EC50 of 2.0 mg NH3/l, and a 14 day LOEC of 3.0 mg NH3/l has been reported for toxicity of ammonia to algae.
[1]https://echa.europa.eu/information-on-chemicals/cl-inventory-database/-/discli/notification-details/32082/948794
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