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EC number: 442-480-8 | CAS number: 182893-11-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- TRIGONOX R-938 is a clear colourless liquid and was treated as 100% pure.
The test substance was hardly soluble in water and was added quantitatively to the test vessels.
A concentration range of 32 to 320 mg/I forming a geometric progression with a factor 1.8 was tested. 100 mg/I was tested in duplicate. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Test System: Micro-organisms in activated sludge
- Source: Municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands.
- Number of micro-organisms: Number of micro-organisms was determined as the amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium.
- Preparation of the sludge: The sludge was coarsely sieved, washed and diluted with tap-water. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.2 g/I of sludge, as used for the test). Before use the pH was checked (measured value: 7.5). The batch of sludge was used on subsequent days (maximum four days), therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of each working day. The sludge was kept aerated at test temperature until use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- The temperature of the sludge, stored overnight in the laboratory, was 23.4°C at the start of the day of testing. Thus, this temperature was 1.4°C above the protocolled range.
Thereafter the sludge was transferred to a climate room. The study was performed in this climate room. The temperature in this room was 19.5°C.
The deviation at the start of the day of testing was considered to have no effect on the outcome of this study. - pH:
- 7.9-8.1
- Dissolved oxygen:
- At the start of the test: 6.5 - 9.7 mg/l
- Nominal and measured concentrations:
- Nominal: 32, 56, 100, 180 and 320 mg/l
- Details on test conditions:
- TEST SYSTEM
- Contact time: 30 minutes, during which aeration and stirring took place.
- Vessels: All glass, 300 ml oxygen bottles and 1 I test bottles.
- Milli-RO / Milli-Q water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ionexchange cartridges (Milli-Q) (Millipore Corp., Bedford, Mass., USA).
- Synthetic sewage feed:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCI
0.4 g CaCI2.2H20
0.2 g MgS04 .7H20
2.8 g K2HP04
Dissolved in 1 I Milli-Q water and filtered.
The pH was 7.0.
- Air supply: Clean, oil-free air.
- Oxygen meter: Oxygen electrode (Tri Ox EO 200, WTW, FRG) supplied with a recorder (Kipp BD40).
- Performance of the test: The synthetic sewage feed (16 ml) and an adequate amount of the test substance were mixed and made up to 300 ml with Milli-RO water. Activated sludge (200 ml) was added and the mixture was aerated in a 1 I bottle during the contact time, using a pipette as an aeration device. Then a well mixed sample of the contents was poured into a 300 ml oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel. Oxygen consumption was measured and recorded for approximately 10 min. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. The pH was determined in the remaining part of the reaction mixture.
This procedure was repeated for all concentrations of the test substance. In each test series two controls without test substance were tested, one at the start and one at the end. Each batch of activated sludge was checked for sensitivity by testing the reference substance 3,5dichlorophenol.
The flasks were filled up to 500 ml final volume. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% C.I. 40-103 mg/l
- Details on results:
- The inhibitory effect of TRIGONOX R-938 on aerobic waste water (activated sludge) bacteria increased with increasing concentration, ranging from 27% inhibition at the lowest concentration tested (32 mg/I) to 92% inhibition at the highest concentration tested (320 mg/I).
The EC-values were determined using linear regression (regression line: Y = 67.38 X -71.91, Y = % inhibition and X = log concentration (mg/I)). - Results with reference substance (positive control):
- Results were as expected.
- Reported statistics and error estimates:
- The EC-values were determined using linear regression (regression line: Y = 67.38 X -71.91, Y = % inhibition and X = log concentration (mg/I)).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 was 65 mg/I with a 95% confidence interval ranging from 40 to 103 mg/l.
- Executive summary:
The influence of the test substance on the respiration rate of activated sludge was investigated after a contact time of 30 minutes.
The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L133, adopted May 30, 1988.
The test substance was hardly soluble in water and was added quantitatively to the test vessels. A concentration range of 32 to 320 mg/I forming a geometric progression with a factor 1.8 was tested. 100 mg/I was tested in duplicate.
The inhibitory effect of the test substance on aerobic waste water (activated sludge) bacteria increased with increasing concentration, ranging from 27% inhibition at the lowest concentration tested (32 mg/I) to 92% inhibition at the highest concentration tested (320 mg/I).
The EC-values were determined using linear regression (regression line: Y =67.38 X -71.91, Y = % inhibition and X = log concentration (mg/I)).
The respiration rates of the controls were within 15% of each other. The EC50 of the reference substance, 3,5-dichlorophenol, was 11 mg/l. Therefore, the test was considered to be valid.
In conclusion, under the conditions of this present test, the test substance was toxic to waste water (activated sludge) bacteria at and above 32 mg/I, the lowest concentration tested.
The EC10 was 16 mg/I with a 95% confidence interval ranging from 9 to 31 mg/l.
The EC20 was 23 mg/I with a 95% confidence interval ranging from 13 to 41 mg/l.
The EC50 was 65 mg/I with a 95% confidence interval ranging from 40 to 103 mg/l.
The EC80 was 180 mg/I with a 95% confidence interval ranging from 111 to 290 mg/l.
Reference
pH, oxygen concentration at the start of measurement and the influence of 3,5-dichlorophenol and the test substance on the oxygen consumption of microbes in activated sludge and percentage inhibition in respiration rate.
Flask |
Conc. Ref./test substance (mg/l) |
Oxygen conc. At the start (mg O2/l) |
Oxygen consumption (mg O2/l/h) |
Inhibition (%) |
pH |
C1 |
- |
8.0 |
34 |
- |
8.0 |
C2 |
- |
6.5 |
37 |
- |
8.1 |
R1 |
3.2 |
8.5 |
31 |
13 |
8.0 |
R2 |
10 |
9.7 |
17 |
52 |
8.1 |
R3 |
32 |
9.5 |
8 |
77 |
8.1 |
T1 |
32 |
7.8 |
26 |
27 |
7.9 |
T2 |
56 |
7.7 |
20 |
44 |
7.9 |
T3 |
100 |
8.5 |
11 |
69 |
8.0 |
T4 |
100 |
8.5 |
13 |
63 |
8.0 |
T5 |
180 |
8.7 |
6 |
83 |
7.9 |
T6 |
320 |
9.4 |
3 |
92 |
8.0 |
Description of key information
The EC10 was 16 mg/L
The EC50 was 65 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 65 mg/L
- EC10 or NOEC for microorganisms:
- 16 mg/L
Additional information
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