ethyl-2-[(ethoxycarbonyl)amino]-4-methyl-5-(4-nitrophenyl)thiophene-3-carboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2022

Report date:

2022

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

results are based on calculated concentration data which are gained out of a non-validated HPLC method

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-[(ethoxycarbonyl)amino]-4-methyl-5-(4-nitrophenyl)thiophene-3-carboxylate

Cas Number:

308831-93-8

Molecular formula:

C17H18N2O6S

IUPAC Name:

Ethyl 2-[(ethoxycarbonyl)amino]-4-methyl-5-(4-nitrophenyl)thiophene-3-carboxylate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Remarks:

results are based on calculated concentration data which are gained out of a non-validated HPLC method

Test solutions

Vehicle:

yes

Remarks:

Elendt M4 medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test species: Daphnia magna Straus
Age: < 24 hours neonates
Origin: National Institute of Environmental Health

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None.

Test conditions

Test temperature:

21 ± 1 °C

pH:

6.953 - 7.273 (pH at the start (d0))
6.589 - 6.788 (pH after 48 h (d2))

Dissolved oxygen:

6.16 mg/l - 8.59 mg/l (oxygen concentration at the start (d0))
8.05 mg/l - 8.71 mg/l (oxygen concentration after 48 h (d2))

Nominal and measured concentrations:

In the test the following calculated concentration was applied: 0.000012; 0.000023; 0.000035; 0.000047 and 0.00007 mg/L (nominal concentrations).

Details on test conditions:

The test was maintained under static conditions for a period of 48 hours.
At the start of the study each test vessels contained 50 ml of test solutions and five young daphnids. The test vessels were covered with plastic lids and were kept at 21 ± 1 °C with a 16/8 hour light/dark cycle.
Each control vessels containing five young daphnids in Elendt M4 medium were prepared in four replicates and kept under the same static conditions.
The number of immobilised and abnormally behaved daphnids were recorded after 24 and 48 hours. Those daphnids were considered to be immobilised that are not able to swim within 15 seconds after gentle agitation of the test vessels.
The daphnids were not fed during the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Remarks on result:

not determinable

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: EC20

Remarks on result:

not determinable

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Remarks on result:

not determinable

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 0 mg/L

Nominal / measured:

nominal

Effect concentrations 5

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0 mg/L

Nominal / measured:

nominal

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to the test results ENTC has no toxic effect on Daphnia immobilisation even at its saturated concentration (0.00007 mg/L).