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EC number: 840-149-6 | CAS number: 308831-95-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Results are based on calculated concentration data which are gained out of a non-validated HPLC method
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl 4-(bromomethyl)-2-[(2,6-difluorophenyl)methyl-ethoxycarbonylamino]-5-(4-nitrophenyl)thiophene-3-carboxylate
- Cas Number:
- 308831-95-0
- Molecular formula:
- C24H21BrF2N2O6S
- IUPAC Name:
- Ethyl 4-(bromomethyl)-2-[(2,6-difluorophenyl)methyl-ethoxycarbonylamino]-5-(4-nitrophenyl)thiophene-3-carboxylate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- Results are based on calculated concentration data which are gained out of a non-validated HPLC method
Test solutions
- Vehicle:
- yes
- Remarks:
- OECD medium
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum and Raphidocelis subcapitata)
Source: Culture Collection of Algae and Protozoa, Scottish Marine Institute (www.ccap.ac.uk)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 ± 2 °C
- pH:
- 7.226 - 7.376 (At the beginning of the test)
7.134 - 7.327 (Average pH at the end of the test) - Dissolved oxygen:
- not applicable
- Nominal and measured concentrations:
- 0.00044; 0.00088; 0.00132; 0.00176 and 0.00264 mg/L (nominal concentration)
- Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.003 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.004 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Nominal / measured:
- nominal
- Remarks on result:
- other: not conclusive
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the test results BNTC did not cause 50% reduction on alga growth rate even at its saturated concentration (0.00264 mg/L). According to the GHS and OECD series on testing and assessment, Number 33 BNTC is not hazardous to the aquatic environment [72 h EC50 (for algae or other aquatic plants) > saturated concentration of BNTC
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