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EC number: 482-140-6 | CAS number: 13641-96-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Feb 2011 – 21 Mar 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- adopted 2008
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 482-140-6
- EC Name:
- -
- Cas Number:
- 13641-96-8
- Molecular formula:
- Hill formula: C6 H7 N O3 CAS formula: C6 H7 N O3
- IUPAC Name:
- 2-isocyanatoethyl prop-2-enoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: All test solutions were taken from all test solutions at the beginning (0 d), immediately prior to the first renewal of the test solution (1 d) and weekly thereafter (10, 21 d).The highest and lowest test conditions were analyzed after pre-treatments.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution of AOI (100 mg/L) was prepared by directly adding 0.010 g of the test item into 100 mL test water. Ultrasonication for 30 min was used to facilitate the dispersion of the test substance. Other test solutions with lower concentrations were prepared by diluting the solution of 100 mg/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): The test species were 6 to 24 hours old at the start of the test.
- Source: All organisms used for a particular test originated from cultures established from the same healthy stock of daphnia.
- Amount: 0.2 – 0.4 mg C/Daphnia
- Frequency: Once every two days.
ACCLIMATION
- Acclimation conditions (same as test or not): The stock animals were maintained in culture conditions (light, temperature, and medium) similar to those to be used in the test.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- see Table 1 in "Any other information on results incl. tables"
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.4 – 7.5
- Dissolved oxygen:
- 85 – 98%
- Nominal and measured concentrations:
- Nominal: 0.1, 0.3, 0.9, 27, 8.1 mg/L
Measured: 0.0773 (nominal 0.1 mg/L), 5.47 (nominal 8.1 mg/L) mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Fill volume: 50 mL
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): The test medium was renwed every 24 hours.
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 50 mL/daphnid
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water which had been dechlorinated for at least 24 h was used.
- Culture medium different from test medium: No
- Intervals of water quality measurement: Besides, oxygen concentration, temperatue, hardness and pH values were measured once a week, in the control(s) and in the highest test substance concentration.
OTHER TEST CONDITIONS
- Photoperiod: 16-hour light and 8-hour dark cycle daily
- Light intensity: Light intensity: 1000 to 1500 lux
- Temperature: 20 ± 1 °C
- Oxygen concentration: 85 – 98%
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Observations: The number of living offspring was counted for each vessel daily. The offspring produced by each parent animal were preferably removed and counted daily from the appearance of the first brood to prevent them consuming food intended for the adult. Time to production of first brood; number of broods per animal.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: The following nominal concentrations of AOI were tested in range-finding test: 0.1 ,1 ,10 ,100 mg/L. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 8.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of control: During the test, all animals in the control were alive and the mean number of live offspring produced per parent animal surviving at the end of the test was >60. The coefficient of variation around the mean number of living offspring produced per parent animal in the control(s) was ≤ 25%.
- Other: Compared with the reproduction of the controls, no significant differences were found in 0.1, 0.3 and 0.9 mg/L treatments (p<0.05). However, reproductions of 2.7 and 8.1 mg/L treatments were inhibited significantly by 10.8% and 21.84% respectively (p<0.05). - Results with reference substance (positive control):
- No reference substance was used
- Reported statistics and error estimates:
- Effect concentrations were calculated using One-Way ANOVA
Any other information on results incl. tables
Table 1: Stability test results of AOI in test media
Nominal Concentration (mg/L) |
Actual concentration (mg/L(TOC)) |
Analyzed concentration (mg/L(TOC)) |
|||||
Day 0 |
Day 1 |
Day 10 |
Day 21 |
||||
fresh |
aged |
fresh |
aged |
fresh |
aged |
||
0 |
- |
0.692 |
0.747 |
0.811 |
0.699 |
0.733 |
0.595 |
0.1 |
0.0773 |
0.797 |
0.790 |
0.800 |
0.805 |
0.797 |
0.796 |
8.1 |
5.47 |
6.22 |
6.33 |
5.89 |
6.14 |
6.22 |
6.31 |
Table 2: Daphnia adult mortality
Nominal concentration (mg/L) |
Exposure time (d) |
|||||||||||||||||||||
|
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
No. of dead adults |
|||||||||||||||||||||
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.9 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2.7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
8.1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
3 |
1 |
0 |
0 |
Table 3: Reproduction inhibition effects
Nominal con. (mg/L) |
Initial No. of adults |
21 d |
|
Mean fecundity (No. of live offspring per adult) |
Inhibition rate |
||
0 |
10 |
61 |
- |
0.1 |
10 |
59 |
3.26% |
0.3 |
10 |
61 |
1.14% |
0.9 |
10 |
57 |
6.51% |
2.7 |
10 |
55 |
10.8% |
8.1 |
10 |
56 |
8.31% |
LOEC (by One-Way ANOVA) |
2.7 mg/L |
||
NOEC (by One-Way ANOVA) |
0.9 mg/L |
||
EC50 |
> 8.1 mg/L (nominal concentration) |
Table 4: Validity criteria for OECD 211
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality of the parent animals in the control (femaleDaphnia) does not exceed 20% at the end of the test. |
During the test, all animals in the control were alive. |
yes |
The mean number of living offspring produced per parent animalsurvivingat the end of the test is ≥ 60 in the control. |
The mean number of live offspring produced per parent animal surviving at the end of the test was 61. |
yes |
Note: The same validity criterion (20%) can be used for accidental and inadvertent parental mortality for the controls as well as for each of the test concentrations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 4 in "Any other information on results incl. tables"
- Conclusions:
- Under semi-static conditions of 24 h-renewal, the effect on the reproductive output of Daphnia magna exposed to the test substance for 21 days was assessed according to OCED 211. Under valid semi-static test conditions (24h-renewal), the LOEC is 2.7 mg/L (nominal concentration). And thus the NOEC is 0.9 mg/L (nominal concentration). The EC50 is greater than 8.1 mg/L (nominal concentration).
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