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EC number: 443-010-4 | CAS number: 53641-10-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-11-23 to 2000-01-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- 1. Copper (II) sulphate pentahydrate instead of Dichlorophenol (unsuitable for routine lab use) 2. Sludge was washed only twice, was deemed sufficient 3. Lower sludge concentration of 3 g/L +- 20 % was deemed sufficient instead of 4 g/L.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 443-010-4
- EC Name:
- -
- Cas Number:
- 53641-10-4
- Molecular formula:
- C14H15ClN2O4
- IUPAC Name:
- N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Disperson treatment: 15 min. ultrasonic treatment at room temperature
Stock solution: None, directly weighing
Test concentrations (nominal): 1000 - 1800 - 3200 - 5800 - 10000 mg/L
Controls: 2 control samples without test item were included in every test series at the beginning and at the end of the test.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The sludge was washed twice with autoclaved tap water and diluted 1:3.
Nutrient solution: Synthetic waste water according to OECD 209
Dry sludge concentration: 2.9 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18 - 22 °C
- pH:
- of activated sludge: 7.21
- Nominal and measured concentrations:
- Nominal: 1000, 1800, 3200, 5800, 10000 mg/L
- Details on test conditions:
- Permanent ventilation
Test formulation: 200 mL inoculum + 16 mL synthetic waste water + 284 mL aqua demin. + directly weighing of the test item
1 replicate per concentration and 2 per control - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate (p.a. MERCK), CAS 7758-99-8, purity 99.0-100.5 % Test concentrations: 58, 100, 180 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7 102 mg/L
- 95% CI:
- 5 452 - 9 251
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 1 178 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- In the reference test the EC50-value was determined by probit analysis. The EC50-value of the reference item is:
EC50= 84 mg/L
The EC50-value of the reference item copper(II)-sulphate-pentahydrate is in the
confidence range of 56 - 146 mg/L. - Reported statistics and error estimates:
- The EC-values of the test and reference item were calculated with probit analysis using softeware SigmaPlot (Windows) rel. 3.02, 1995, JANDEL SCIENTIFIC. Probit values were taken from WEBER (1986). Confidence intervals were calculated according to a standard procedure (BREITIG & TÜMPLING 1982).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The quality criteria were fulfilled: - The two control respiration rates differ not more than 15 %. - The EC50(3 hours) of copper(I1)sulphate pentahydrate is in the accepted range of 56 - 146 mg/L
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 and GLP principles was performed about 3 h with the test item CL-DAEP (batch-no. 90003CCL) on November 30, 1999 at the test facility DR. U. NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE.
Test system was activated sludge of the municipal treatment plant of D-31137Hildesheim. The test method was static. Nominal concentrations of the test item were as follows:1000- 1800 - 3200 - 5800 - 10000 mg/L
The respiration rates of control, reference and test item were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates.
The EC20 and EC50 values were determinable in the tested concentration range . In order to check the activity of the test system and the test conditions a reference test is carried out once every 7 days with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity determined. The EC50 value for the reference item was 84 mg/L tested December 02, 1999. The quality criteria of the test guideline were fulfilled.
EC-values with confidence interval of Cl-DAEP:
EC20 = 1178 mg/L
EC50 = 7102 mg/L (95% CI = 5452 - 9251 mg/L)
EC80 = > 10000 mg/L
The test item CL-DAEP is not toxic in concentrations 5_ 1178 mg/L to activated sludge of a municipal sewage treatment plant.
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