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EC number: 218-259-5 | CAS number: 2095-06-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP study performed according to OECD Guideline 203 [1992] and EEC directive 92/69 Part C.1 [1992]. No analytical control of test concentrations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- EC Number:
- 225-716-2
- EC Name:
- p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- Cas Number:
- 5026-74-4
- Molecular formula:
- C15H19NO4
- IUPAC Name:
- 4-(oxiran-2-ylmethoxy)-N,N-bis(oxiran-2-ylmethyl)aniline
- Test material form:
- liquid: viscous
- Details on test material:
- - Substance type: organic
- Storage condition of test material: In refrigerator (2-8°C) in the dark
- Stability under test conditions: stable
- Descrition : Clear yellow liquid (Determined at NOTOX)
- Analytical purity: >95%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material dissolved by stirring in acetone to create stock solutions. 0.2 ml of solvent was added to 2 l of test medium and mixed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.01%
- Evidence of undissolved material (e.g. precipitate, surface film, etc): All final test solutions appeared clear and without precipitation.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp (Cyprinus carpio. Teleostei, Cyprinidae) (Linnaeus, 1758)
- Reason for selection : This system has been selected as an internationally accepted species.
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, NL
- Total fish used : 79
- Length at study initiation (length definition, mean, range and SD): 1.96 +/- 0.10 cm
- Weight at study initiation (mean and range, SD): 0.21 +/- 0.03 g
- Method of breeding: F1 from a single partent-pair bred in UV-treated water
- Feeding during test : no feeding from 24 hours prior to test and during total test period
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same, with feeding; test without feeding
- Type and amount of food: Trouvit, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): < 5% mortality during the 7 days prior the start of the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 20.5 deg C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 9.2 - 8.7 mg/L
- Salinity:
- Iso medium (Ca++, Mg++, Na+, K+, Cl-, SO4 2-, HCO3-); total 351 mg / L
- Nominal and measured concentrations:
- Nominal: 10, 5.6, 3.2, 1.8, 1.0 mg/L test material plus a solvent and a negative control
Analytics have not been performed - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 liters
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass
- Aeration: continously
- No. of organisms per vessel: 7 fish per concentration and control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.74 g fish / litre, e.g. 7 fish per 2 litres of test medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Millipore reverse osmosis tap water, supplemented with salts to generate iso medium
- Particulate matter: none observed
- Intervals of water quality measurement: Oxygen, pH, temperature: daily // Ammonia, Nitrite, Nitrate once a week.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours / day
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality (at 2, 24, 48, 72 and 96 hours after start of treatment)
- disturbance of swimming behaviour (at 2, 24, 48, 72 and 96 hours after start of treatment)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L in Rang-Finding study
- Results used to determine the conditions for the definitive study: 100% mortality at 100 and 10 mg/L, 0% mortality at 1 mg/L in RF study - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- >= 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- <= 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Swimming behaviour disturbed at 48 hours of 3 moribund fish at the concentration of 5.6 mg/l
- Observations on body length and weight: no data
- Other biological observations: no data
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no observations
- Effect concentrations exceeding solubility of substance in test medium: no data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 in carp after 96 hours of treatment is 4.2 mg/L with 0% mortality at 3.2 mg/L and 100% mortality at 5.6 mg/L.
- Executive summary:
In the acute toxicity study in carp, each 7 fish were exposed for 96 hours to diluted solutions of TK 12576. Acetone was used as a solvent at 0.01% in the test medium. The concentration range tested was selected on the basis of results obtained in a range finder study. The LC50 after 96 hours of treatment was 4.2 mg/L with 0% mortality at 3.2 mg/L and 100% mortality at 5.6 mg/L.
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