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EC number: 915-008-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- March 2006, corrected July 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: the control, the lowest, the highest concentrations and a concentration around the expected EC50 concentration: 0, 3.2, 100 and 320 mg/L
- Sampling method: All the solutions will be analysed taken directly from the starting solutions without algae.
- Sample storage conditions before analysis: All the solutions were kept in refrigerator or freeze in order to be analyzed if necessary or relevant. - Vehicle:
- no
- Details on test solutions:
- According to the sponsor, the test item is soluble in water. The dilution water was the algae test medium, a weakly saline aqueous medium.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: /
- Strain: CCAP 278.4
- Source (laboratory, culture collection): laboratory
- Age of inoculum (at test initiation): not specified
- Method of cultivation: The algae cultures are stored in the laboratory on a solid medium (LC/agar Petri plates) and in a liquid medium with regular sub-culturing (every week).
ACCLIMATION
no acclimation - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.6 – 22.9°C
- pH:
- 7.1-7.7
- Nominal and measured concentrations:
- According to the results of the preliminary test, we proposed to test five concentrations for this study: 3.2 – 10.0 – 32.0 – 100.0 and 320.0 mg/L.
Measured concentrations: see table - Details on test conditions:
- TEST SYSTEM
- Test vessel: erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: initial concentration of 10^4 cells/mL
- Control end cells density:
- No. of organisms per vessel: initial concentration of 10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): not relevant
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: not applicable
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuously illuminated
- Light intensity and quality: 6035 Lux
- Salinity (for marine algae): not relevant
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
The algae growth inhibition in relation to the control was evaluated over a period of 72 hours. The algae cells per unit volume were measured during the test at 24, 48 and 72 hours with a particle counter (Beckman Coulter Z2) and/ or under microscope with Malassez counting cell.
- Chlorophyll measurement: no
TEST CONCENTRATIONS
0 - 3.2 – 10.0 – 32.0 – 100.0 and 320.0 mg/L.
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: yes
- Test concentrations:
range finding test: 0, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Results with reference substance (positive control):
- The last algae growth inhibition test performed with the reference item K2Cr2O7 (September 18 2017) gave an ErC50(0-72h) = 1.11 mg/L (0.98 – 1.24), indicating that the algae sensitivity is correct (within the accepted interval of 0.65 to 1.73 mg/L fixed by the International Standard NF EN ISO 8692 from Mai 2012 as mentioned in the OECD Guideline) and in accordance with previous data obtained by the laboratory.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results obtained for the test item “REACTION MIXTURE OF CuDTPA and CuHEEDTA” on the toxicological endpoint “inhibition of growth rate” indicate an ErC50-(0-72h) = 17.9 mg/L [16.3 – 19.5] value expressed in nominal test item concentration.
The results obtained for the test item “REACTION MIXTURE OF CuDTPA and CuHEEDTA” on the toxicological endpoint “Yield inhibition” indicate an EyC50-(0-72h) lower than 3.2 mg/L (lowest tested concentration), value expressed in nominal test item concentration.
In the control. the increase of the biomass during 72 hours was determined to be higher than factor of 16.
The coefficients of variation of daily section-by-section specific growth rate and of average specific growth rate were determined respectively to be less than 35% and 7% in the control.
The validity criteria were successful fulfilled. - Executive summary:
The aim of this study was to determine the effects of the test item "REACTION MIXTURE OF CuDTPA and CuHEEDTA" on the growth of the unicellular green algaPseudokirchneriella subcapitata, according to the OECD 201 guideline (March 2006, corrected July 2011).
The study was carried out in accordance with the study plan n°17/1108F/b dated from Novembre 6, 2017.
There was no deviation and no amendment during this study.
The results of this study are summarized below:
Test item REACTION MIXTURE OF CuDTPA and CuHEEDTA
Test system Pseudokirchneriella subcapitata
Exposure duration 72 hours
Test concentrations Control – 3.2 – 10.0 – 32.0 - 100.0 and 320.0 mg/L
Incubator Temperature 22.6 – 22.9°C ; shaking at 120 rpm; light intensity: 6035 Lux
Measurement of cell growth Beckman Coulter Z2 and Malassez counting cell
Results
The results obtained for the test item “REACTION MIXTURE OF CuDTPA and CuHEEDTA” on the toxicological endpoint “inhibition of growth rate” indicate an ErC50-(0-72h) = 17.9 mg/L [16.3 – 19.5], value expressed in nominal test item concentration.
The results obtained for the test item “REACTION MIXTURE OF CuDTPA and CuHEEDTA” on the toxicological endpoint “Yield inhibition” indicate an EyC50-(0-72h) lower than 3.2 mg/L (lowest tested concentration), value expressed in nominal test item concentration.
In the control. the increase of the biomass during 72 hours was determined to be higher than factor of 16.
The coefficients of variation ofdaily section-by-section specific growth rateand ofaverage specific growth ratewere determined respectively to be less than 35% and 7% in the control.
The validity criteria were successful fulfilled.
Reference
1 . Calculation of the ErC50 (0-72h)
Inhibitions of the average specific growth rate in comparison to the Control are presented in the following table:
Nominal test item concentration |
Algae *104/mL at 0 hour |
Average growth rate µ0-72h |
Inhibition growth rate (% Ir) |
Average inhibition of growth rate (%) |
Coefficient of variation |
Significance |
|
Control |
a |
1 |
1,791 |
Not applicable |
2.0 |
- |
|
b |
1,838 |
||||||
c |
1,872 |
||||||
d |
1,776 |
||||||
e |
1,795 |
||||||
f |
1,812 |
||||||
3.2 mg/L |
a |
1 |
1,565 |
13,8 |
13,7
|
Not required |
* |
b |
1,560 |
14,1 |
|||||
c |
1,577 |
13,1 |
|||||
10.0 mg/L |
a |
1 |
1,128 |
37,9 |
40,6
|
Not required |
* |
b |
1,058 |
41,7 |
|||||
c |
1,042 |
42,6 |
|||||
32.0 mg/L |
a |
1 |
0,732 |
59,7 |
61,4
|
Not required |
* |
b |
0,672 |
63,0 |
|||||
c |
0,693 |
61,8 |
|||||
100.0 mg/L |
a |
1 |
0,536 |
70,5 |
72,5
|
Not required |
* |
b |
0,478 |
73,7 |
|||||
c |
0,478 |
73,7 |
|||||
320.0 mg/L |
a |
1 |
0,355 |
80,5 |
80,2 |
Not required |
* |
b |
0,343 |
81,1 |
|||||
c |
0,377 |
79,2 |
- : not relevant
ns : not significantly different compared to the Control. Dunnet test. α =0.05
* : significantly different compared to
the Control. Dunnett test. α=0.05
The average specific growth rate (µ) was determined according to the OECD guideline.
Statistical significance was determined with the Dunnett test (α=5%) from raw data with the scientific software Minitab 17. The NOEC/LOEC were determined as:
NOEC |
LOEC |
Not determined < 3.2 mg/L |
3.2 mg/L |
A regression model (non linear) was relevant to calcultate an ErC50-(0-72h) . The equation is:
Inhibition Growth rate (%) = 82.8045*Test item concentration / (11.7123 + Test item concentration)
The results obtained for thetest item “REACTION MIXTURE OF CuDTPA and CuHEEDTA”on the toxicological endpoint “inhibition of growth rate” indicate anErC50-(0-72h)= 17.9 mg/L [16.3 – 19.5]value expressed in nominal test item concentration.
The results obtained for thetest item “REACTION MIXTURE OF CuDTPA and CuHEEDTA”on the toxicological endpoint “inhibition of growth rate” indicate anErC10-(0-72h)lower than 3.2 mg/L (lowest tested concentration) value expressed in nominal test item concentration.
2. Calculation of the EyC50 (0-72h)
Inhibitions of the yield in comparison to the Control are presented in the following table:
Nominal test item concentration |
[algae] *104/mL at 0 hour |
[algae] *104/mL at 72 hours |
Yield (*104/mL) |
Average yield (*104/mL) |
Yield inhibition (Iy) (%) |
||
Per replicate |
Average/ replicate |
||||||
Control |
a |
1 |
215,5 |
214,5 |
231,1
|
Not Applicable |
|
b |
248,2 |
247,2 |
|||||
c |
275,2 |
274,2 |
|||||
d |
206,3 |
205,3 |
|||||
e |
217,8 |
216,8 |
|||||
f |
229,6 |
228,6 |
|||||
3.2 mg/L |
a |
1 |
109,5 |
108,5 |
109,3
|
53,1 |
52,7
|
b |
107,9 |
106,9 |
53,7 |
||||
c |
113,5 |
112,5 |
51,3 |
||||
10.0 mg/L |
a |
1 |
29,5 |
28,5 |
24,4
|
87,7 |
89,4
|
b |
23,9 |
22,9 |
90,1 |
||||
c |
22,8 |
21,8 |
90,6 |
||||
32.0 mg/L |
a |
1 |
9,0 |
8,0 |
7,2
|
96,5 |
96,9
|
b |
7,5 |
6,5 |
97,2 |
||||
c |
8,0 |
7,0 |
97,0 |
||||
100.0 mg/L |
a |
1 |
5,0 |
4,0 |
3,5
|
98,3 |
98,5
|
b |
4,2 |
3,2 |
98,6 |
||||
c |
4,2 |
3,2 |
98,6 |
||||
320.0 mg/L |
a |
1 |
2,9 |
1,9 |
1,9 |
99,2 |
99,2 |
b |
2,8 |
1,8 |
99,2 |
||||
c |
3,1 |
2,1 |
99,1 |
The equation of the regression (non linear regression) is the following:
Yield inhibition (%) = 102.793 * Test item concentration / (2.50896 + Test item concentration)
The results obtained for thetest item “REACTION MIXTURE OF CuDTPA and CuHEEDTA”on the toxicological endpoint “Yield inhibition” indicate anEyC50-(0-72h)lower than 3.2 mg/L (lowest tested concentration),value expressed in nominal test item concentration.
Description of key information
Chelating agents are known to make metal ions less bioavailable in freshwater (Sorvari et al. 1996).
The aquatic toxicity of the Reaction mixture of CuDTPA and CuHEEDTA is thus expected to be lower compared to more bioavailble copper compounds.
Based on an OECD 201 study, the 72-h ErC50 of 17.9 mg/L was determined.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 17.9 mg/L
Additional information
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