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EC number: 227-497-9 | CAS number: 5858-81-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 July 2017 - 20 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23
- Version / remarks:
- 2000
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Stability in water: stable
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken from the test concentration and the control according to the schedule below:
Frequency at t=0 h and t=96 h
Volume 2.0 mL
Storage Samples were stored in a freezer (≤-15°C)
In addition, the filter containing the undissolved residue was kept for possible analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct application to the test medium.
All test solutions were individually prepared at loading rates of 1.0, 10 and 100 mg/L. A one day period of magnetic stirring was applied to ensure maximum dissolution of the test item in medium. Thereafter, the aqueous Water Accommodated Fractions (WAFs) were filtered through a 0.45 µm membrane filter and used as test concentrations.
- Controls: Test medium without test item or other additives.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Solutions were checked for Tyndall effect with a laser pen. No distraction of the beam was observed. All test solutions were clear and colorless at the end of the preparation procedure. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: carp
- Strain: Linnaeus, 1758
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
- Age at study initiation: not indicated
- Length at study initiation: 2.7 ± 0.2 cm
- Weight at study initiation: 0.21 ± 0.07 g
- Method of breeding: in UV-treated water
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: daily with pelleted fish food (Essence (300-500 um), Coppens International bv, Helmond, The Netherlands)
- Health during acclimation: mortality during the seven days prior to the start of the test was less than 5%
FEEDING DURING TEST: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg CaCO3/L
- Test temperature:
- 21°C
- pH:
- 7.9 at test initiation
7.5-7.8 throughout the remainder of the test - Dissolved oxygen:
- 9.0 and 8.9 at test initiation
6.5-9.0 throughout the remainder of the test. - Nominal and measured concentrations:
- Nominal concentration: WAF prepared at 100 mg/L
Measured concentration: 0.151 mg/L at t=0 and 0.149 mg/L at t=96 (99% of initial measured concentration at t=0) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 6.5 litres, all-glass, fill volume: 5.0 litres
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.29 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Test medium used: adjusted ISO medium according to OECD guideline 203
- Source/preparation of dilution water: tap-water purified by reverse osmosis
- Culture medium different from test medium: no
- Intervals of water quality measurement:
* Conductivity, pH, nitrate, nitrite and ammonia concentration: once a week
* Temperature: continuous
* pH and temperature were measured before transferring the fish to the test system
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours, daily
EFFECT PARAMETERS MEASURED: mortality at t= 2 1/2, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: based on the results of a dose-range finding test, a limit test was performed.
- Range finding study
- Test concentrations: WAFs prepared at 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes, since no mortality or clinical effects were observed at any of the test concentrations during the test period, the LC50 was expected to exceed the highest WAF prepared at a nominal loading rate of 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol (February 2017)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.15 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Details on results:
- - No mortality occured during the test period. Mortality was not recorded at t=48. However, since no mortality occured during the test period, this deviation of the study plan does not influence the results of the study.
- No abnormalities were observed during the final test. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mortality: all carp exposed to a concentration of 0.46 mg/L died within 24 hours, while no mortality occurred at 0.10 and 0.22 mg/L.
- LC50: 0.32 mg/L (95 % confidence interval between 0.22 and 0.46 mg/L)
- Other: since the results with the reference substance were within the historical data range, the carp used in this study are considered to be of anticipated sensitivy. - Validity criteria fulfilled:
- yes
- Remarks:
- see 'overall remarks' section.
- Conclusions:
- Based on the results of an acute toxicty study in fish, according to OECD guideline 203 and GLP principles, the LC50 of D&C Red 6 was >0.15 mg/L, which was considered to be the maximum soluble concentration of the test item in test medium.
- Executive summary:
An acute toxicity study in fish was performed, according to OECD guideline 203 and GLP principles, to assess the effect of D&C Red 6 on carp after 96 hours of exposure. The test solutions were prepared indivudually by adding the test item directly to the test medium, stirring for one day and subsequently filtering the WAFs. Loading rates of 1.0, 10 and 100 mg/L were used in a range-finding study. Since no effects were observed in the range-finding study, the highest concentration was used to determined the final effect concentrations. Seven fish (Cyprinus carpio) were exposed to the limit concentration for an exposure period of 96 hours in which mortality was recorded at t=2 1/2, 24, 72 and 96 hours. Samples of the test solution were analysed, which showed actual exposure concentrations of 0.151 mg/L at t=0 and 0.149 mg/L at t=96. Since the test item remained stable during the exposure period (99% of initial), the effect concentrations was based on the measured concentration.
No mortality or other abnormalities were observed. Therefore, the LC50 was determined to exceed the exposure concentration of 0.15 mg/L, which was considered to be the maximum soluble concentration of the test item in test medium.
All acceptability criteria were met and the study was considered to be valid.
Reference
Description of key information
An acute toxicity study in fish was performed, according to OECD guideline 203 and GLP principles, to assess the effect of D&C Red 6 on carp after 96 hours of exposure. The test solutions were prepared indivudually by adding the test item directly to the test medium, stirring for one day and subsequently filtering the WAFs. Loading rates of 1.0, 10 and 100 mg/L were used in a range-finding study. Since no effects were observed in the range-finding study, the highest concentration was used to determined the final effect concentrations. Seven fish (Cyprinus carpio) were exposed to the limit concentration for an exposure period of 96 hours in which mortality was recorded at t=2 1/2, 24, 72 and 96 hours. Samples of the test solution were analysed, which showed actual exposure concentrations of 0.151 mg/L at t=0 and 0.149 mg/L at t=96. Since the test item remained stable during the exposure period (99% of initial), the effect concentrations was based on the measured concentration.
No mortality or other abnormalities were observed. Therefore, the LC50 was determined to exceed the exposure concentration of 0.15 mg/L, which was considered to be the maximum soluble concentration of the test item in test medium.
All acceptability criteria were met and the study was considered to be valid.
Key value for chemical safety assessment
Additional information
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