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EC number: 209-269-0 | CAS number: 564-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- non-GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- [3aR-(3aα,5aβ,9aα,9bβ)]decahydro-3a,6,6,9a-tetramethylnaphth[2,1-b]furan-2(1H)-one
- EC Number:
- 209-269-0
- EC Name:
- [3aR-(3aα,5aβ,9aα,9bβ)]decahydro-3a,6,6,9a-tetramethylnaphth[2,1-b]furan-2(1H)-one
- Cas Number:
- 564-20-5
- Molecular formula:
- C16H26O2
- IUPAC Name:
- [3aR-(3aα,5aβ,9aα,9bβ)]decahydro-3a,6,6,9a-tetramethylnaphth[2,1-b]furan-2(1H)-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Storage conditions: stored at approximately 4°C (refrigerator) in the dark under N2 conditions.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- A mixed population of activated sewage sludge micro-organisms (Test System) was obtained on January 30th, 2017 from the secondary treatment stage of the sewage treatment plant at Villette (STEP Villette, av. de Thônex 105, 1226 THONEX (Geneva, Switzerland)), which treats predominantly domestic sewage.
The culture medium used in this study (see Appendix 1) was that recommended in the OECD Guideline N° 301F.
The sample of activated sewage sludge was maintained on continuous aeration upon receipt. A sample of the activated sewage sludge was washed three times by settlement (centrifuge: Heittich rotenta 460 RS) and suspension in culture medium. To remove any excessive amounts of Dissolved Organic Carbon (DOC) that may have been present, the solution was stirred and maintained on with pure oxygen at room temperature. Determination of dry weight is made to inoculate final solution with 30mg/L dry weight activated sludge. - Duration of test (contact time):
- 60 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 102.5 mg/L
- Initial conc.:
- 97.5 mg/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The test material (test 1) was dispersed directly in culture medium. An amount of test material (20.5mg) was dispersed in 200mL of culture medium inoculated to give the test concentration of 102.5mg /L.
The test material (test 2) was dispersed directly in culture medium. An amount of test material (19.5mg) was dispersed in 200mL of culture medium inoculated to give the test concentration of 97.5mg /L.
Analysis of the concentration, homogeneity and stability of the test material in the test solutions were not appropriate to the test guideline.
The culture medium used in this study was that recommended in the OECD Guideline N° 301F.
On Day 0, the following solutions were inoculated in 1000mL glass culture vessels (total volume when full 1145mL) each containing 200mL of solution:
A control, in duplicate, consisting of inoculated culture medium.
The test material (SCLAREOIDE NEW CRIST), in duplicate, in inoculated culture medium.
The reference material (Sodium Benzoate) in inoculated culture medium.
The test material and the reference material in inoculated culture medium as a toxicity control.
The system consists of a sample flask sealed by a sensor head/CO2 trap in a temperature controlled incubator. The samples were stirred for the duration of the study with a magnetically coupled stirrer.
As biodegradation progresses, the micro-organisms convert oxygen to carbon dioxide which is absorbed into NaOH causing a net reduction in gas pressure within the sample flask.
WTW oxitopC calculate automatically the consumption of oxygen.
The test was conducted in diffuse light at a temperature of 22°C +/- 1°C.
The daily Biochemical Oxygen Demand (BOD) values for the control, test and reference materials and the toxicity control have available below.
The BOD was adjusted automatically at the temperature measurement.
The total amount of oxygen required to oxidise a chemical completely is calculated from the molecular formula (assuming no nitrification occurs) and expressed as mg oxygen required per mg test item as described in the OECD Guideline N° 301F.
The biodegradability exerted after each period was calculated as :
BOD= (mgO2/luptake by test substance -O2/l uptake by blank)/ mg test substance/lin vessel = mg02/mg test substance
The percentage biodegradation of the test items and of the reference item sodium benzoate was calculated as:
% biodegrdation= BOD(mgO2/mg test substance)/ThOD(mgO2/mg test substance) x100
Validation criteria
A test is considered valid if the difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%, and if the percentage degradation of the reference compound has reached the pass levels by day 14. If either of these conditions is not met, the test should be repeated. Because of the stringency of the methods, low values do not necessarily mean that the test substance is not biodegradable under environmental conditions, but indicates that more work will be necessary to establish biodegradability.
The BOD of the inoculated culture control medium (control) is normally 20 to 30mgO2/L and should not exceed 60mgO2/L after 28 days. Value higher than 60mgO2/L requires critical examination of the data and experimental technique. If the pH value is outside the range 6-8.5 and the oxygen consumption by the test substance is less than 60%, the test should be repeated with a lower concentration of test substance.
The results of the degradation test are considered valid if in the same test the reference material yields 60% degradation by Day 14.
The test material may be considered to be readily biodegradable if 60% degradation is attained after 28 days. This level of degradation must be reached within 10 days of biodegradation exceeding 10%.
The toxicity control should attain 25% degradation by Day 14 for the test material to be considered as non-inhibitory.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- The test material (test 1) attained 45% degradation after 28 days, 62% degradation after 60 days.
The test material (test 2) attained 61 % degradation after 28 days days and didn’t satisfy the 10-day window validation criterion, 72% degradation after 60 days.
Therefore the test material cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline N° 301F.
The toxicity test attained 49% degradation after 28 days thereby confirming that the test material was not toxic to the sewage treatment micro-organisms used in the study.
Sodium Benzoate attained 78 % degradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- test 1
- Value:
- 45
- Sampling time:
- 28 d
- Remarks on result:
- other: 62% after 60 days
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- test 2
- Value:
- 61
- Sampling time:
- 28 d
- Remarks on result:
- other: 72% after 60 days
- Details on results:
- pH comprised between 7.37 and 7.63
BOD5 / COD results
- Results with reference substance:
- Sodium Benzoate attained 78 % degradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
Any other information on results incl. tables
Table 5.2.1/1: Cumulative Biochemical Oxygen Demand (mg O2/L) in test flask
days |
min |
bl1 |
bl2 |
test1 |
test2 |
reference |
toxicity |
abiotic |
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2880 |
6.2 |
0 |
6.2 |
6.2 |
80.6 |
86.8 |
0 |
4 |
5760 |
6.2 |
0 |
6.2 |
6.2 |
118 |
118 |
6.2 |
6 |
8640 |
6.2 |
6.2 |
31 |
55.8 |
130 |
136 |
6.2 |
8 |
11520 |
6.2 |
6.2 |
49.6 |
80.6 |
136 |
167 |
6.2 |
10 |
14400 |
12.4 |
6.2 |
62 |
99.3 |
130 |
186 |
0 |
12 |
17280 |
12.4 |
6.2 |
74.4 |
118 |
136 |
205 |
0 |
14 |
20160 |
12.4 |
12.4 |
86.8 |
130 |
143 |
223 |
6.2 |
16 |
23040 |
6.2 |
12.4 |
93.1 |
136 |
155 |
242 |
6.2 |
18 |
25920 |
18.6 |
18.6 |
112 |
155 |
149 |
242 |
0 |
20 |
28800 |
12.4 |
12.4 |
112 |
155 |
161 |
261 |
6.2 |
22 |
31680 |
18.6 |
24.8 |
130 |
167 |
161 |
273 |
12.4 |
24 |
34560 |
18.6 |
24.8 |
136 |
174 |
167 |
279 |
12.4 |
26 |
37440 |
24.8 |
24.8 |
143 |
186 |
161 |
279 |
6.2 |
28 |
40320 |
18.6 |
24.8 |
149 |
186 |
167 |
285 |
6.2 |
30 |
43200 |
18.6 |
24.8 |
155 |
192 |
161 |
292 |
6.2 |
32 |
46080 |
18.6 |
24.8 |
155 |
199 |
167 |
298 |
6.2 |
34 |
48960 |
24.8 |
24.8 |
161 |
205 |
161 |
298 |
6.2 |
36 |
51840 |
18.6 |
24.8 |
161 |
205 |
167 |
304 |
6.2 |
38 |
54720 |
24.8 |
24.8 |
167 |
217 |
167 |
310 |
6.2 |
40 |
57600 |
24.8 |
24.8 |
174 |
211 |
161 |
310 |
6.2 |
42 |
60480 |
24.8 |
24.8 |
174 |
217 |
167 |
316 |
12.4 |
44 |
63360 |
24.8 |
24.8 |
174 |
223 |
167 |
310 |
6.2 |
46 |
66240 |
24.8 |
24.8 |
180 |
223 |
161 |
316 |
6.2 |
48 |
69120 |
24.8 |
24.8 |
180 |
223 |
174 |
323 |
12.4 |
50 |
72000 |
24.8 |
24.8 |
186 |
223 |
174 |
329 |
12.4 |
52 |
74880 |
24.8 |
24.8 |
192 |
230 |
167 |
329 |
6.2 |
54 |
77760 |
24.8 |
24.8 |
192 |
230 |
174 |
335 |
6.2 |
56 |
80640 |
24.8 |
24.8 |
192 |
230 |
174 |
335 |
12.4 |
58 |
83520 |
24.8 |
24.8 |
199 |
236 |
167 |
335 |
6.2 |
60 |
86400 |
24.8 |
24.8 |
199 |
230 |
167 |
335 |
6.2 |
Table 5.2.1/2: Percentage biodegradation in Test 1
days |
blanc 1 |
blanc 2 |
average |
test1 |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.0 |
0 |
|
2 |
6.2 |
0 |
3.1 |
6.2 |
0.6 |
0.0 |
1 |
|
4 |
6.2 |
0 |
3.1 |
6.2 |
0.6 |
0.0 |
1 |
|
6 |
6.2 |
6.2 |
6.2 |
31 |
5.0 |
0.2 |
9 |
|
8 |
6.2 |
6.2 |
6.2 |
49.6 |
8.7 |
0.4 |
15 |
|
10 |
12.4 |
6.2 |
9.3 |
62 |
10.5 |
0.5 |
19 |
|
12 |
12.4 |
6.2 |
9.3 |
74.4 |
13.0 |
0.6 |
23 |
|
14 |
12.4 |
12.4 |
12.4 |
86.8 |
14.9 |
0.7 |
26 |
|
16 |
6.2 |
12.4 |
9.3 |
93.1 |
16.8 |
0.8 |
30 |
|
18 |
18.6 |
18.6 |
18.6 |
112 |
18.7 |
0.9 |
33 |
|
20 |
12.4 |
12.4 |
12.4 |
112 |
19.9 |
1.0 |
35 |
|
22 |
18.6 |
24.8 |
21.7 |
130 |
21.7 |
1.1 |
38 |
|
24 |
18.6 |
24.8 |
21.7 |
136 |
22.9 |
1.1 |
41 |
|
26 |
24.8 |
24.8 |
24.8 |
143 |
23.6 |
1.2 |
42 |
|
28 |
18.6 |
24.8 |
21.7 |
149 |
25.5 |
1.2 |
45 |
|
30 |
18.6 |
24.8 |
21.7 |
155 |
26.7 |
1.3 |
47 |
|
32 |
18.6 |
24.8 |
21.7 |
155 |
26.7 |
1.3 |
47 |
|
34 |
24.8 |
24.8 |
24.8 |
161 |
27.2 |
1.3 |
48 |
|
36 |
18.6 |
24.8 |
21.7 |
161 |
27.9 |
1.4 |
49 |
|
38 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
50 |
|
40 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
53 |
|
42 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
53 |
|
44 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
53 |
|
46 |
24.8 |
24.8 |
24.8 |
180 |
31.0 |
1.5 |
55 |
|
48 |
24.8 |
24.8 |
24.8 |
180 |
31.0 |
1.5 |
55 |
|
50 |
24.8 |
24.8 |
24.8 |
186 |
32.2 |
1.6 |
57 |
|
52 |
24.8 |
24.8 |
24.8 |
192 |
33.4 |
1.6 |
59 |
|
54 |
24.8 |
24.8 |
24.8 |
192 |
33.4 |
1.6 |
59 |
|
56 |
24.8 |
24.8 |
24.8 |
192 |
33.4 |
1.6 |
59 |
|
58 |
24.8 |
24.8 |
24.8 |
199 |
34.8 |
1.7 |
62 |
|
60 |
24.8 |
24.8 |
24.8 |
199 |
34.8 |
1.7 |
62 |
Table 5.2.1/3: Percentage Biodegradation in Test 2
days |
blank 1 |
blank 2 |
average |
test2 |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.0 |
0 |
|
2 |
6.2 |
0 |
3.1 |
6.2 |
0.6 |
0.0 |
1 |
|
4 |
6.2 |
0 |
3.1 |
6.2 |
0.6 |
0.0 |
1 |
|
6 |
6.2 |
6.2 |
6.2 |
55.8 |
9.9 |
0.5 |
19 |
|
8 |
6.2 |
6.2 |
6.2 |
80.6 |
14.9 |
0.8 |
28 |
|
10 |
12.4 |
6.2 |
9.3 |
99.3 |
18.0 |
0.9 |
34 |
|
12 |
12.4 |
6.2 |
9.3 |
118 |
21.7 |
1.1 |
41 |
|
14 |
12.4 |
12.4 |
12.4 |
130 |
23.5 |
1.2 |
44 |
|
16 |
6.2 |
12.4 |
9.3 |
136 |
25.3 |
1.3 |
47 |
|
18 |
18.6 |
18.6 |
18.6 |
155 |
27.3 |
1.4 |
51 |
|
20 |
12.4 |
12.4 |
12.4 |
155 |
28.5 |
1.5 |
53 |
|
22 |
18.6 |
24.8 |
21.7 |
167 |
29.1 |
1.5 |
54 |
|
24 |
18.6 |
24.8 |
21.7 |
174 |
30.5 |
1.6 |
57 |
|
26 |
24.8 |
24.8 |
24.8 |
186 |
32.2 |
1.7 |
60 |
|
28 |
18.6 |
24.8 |
21.7 |
186 |
32.9 |
1.7 |
61 |
|
30 |
18.6 |
24.8 |
21.7 |
192 |
34.1 |
1.7 |
64 |
|
32 |
18.6 |
24.8 |
21.7 |
199 |
35.5 |
1.8 |
66 |
|
34 |
24.8 |
24.8 |
24.8 |
205 |
36.0 |
1.8 |
67 |
|
36 |
18.6 |
24.8 |
21.7 |
205 |
36.7 |
1.9 |
68 |
|
38 |
24.8 |
24.8 |
24.8 |
217 |
38.4 |
2.0 |
72 |
|
40 |
24.8 |
24.8 |
24.8 |
211 |
37.2 |
1.9 |
69 |
|
42 |
24.8 |
24.8 |
24.8 |
217 |
38.4 |
2.0 |
72 |
|
44 |
24.8 |
24.8 |
24.8 |
223 |
39.6 |
2.0 |
74 |
|
46 |
24.8 |
24.8 |
24.8 |
223 |
39.6 |
2.0 |
74 |
|
48 |
24.8 |
24.8 |
24.8 |
223 |
39.6 |
2.0 |
74 |
|
50 |
24.8 |
24.8 |
24.8 |
223 |
39.6 |
2.0 |
74 |
|
52 |
24.8 |
24.8 |
24.8 |
230 |
41.0 |
2.1 |
77 |
|
54 |
24.8 |
24.8 |
24.8 |
230 |
41.0 |
2.1 |
77 |
|
56 |
24.8 |
24.8 |
24.8 |
230 |
41.0 |
2.1 |
77 |
|
58 |
24.8 |
24.8 |
24.8 |
236 |
42.2 |
2.2 |
79 |
|
60 |
24.8 |
24.8 |
24.8 |
230 |
41.0 |
2.1 |
77 |
Table 5.2.1/4: Percentage Biodegradation in Reference
days |
blank 1 |
blank 2 |
average |
ref |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.0 |
0 |
|
2 |
6.2 |
0 |
3.1 |
80.6 |
15.5 |
0.8 |
47 |
|
4 |
6.2 |
0 |
3.1 |
118 |
23.0 |
1.1 |
69 |
|
6 |
6.2 |
6.2 |
6.2 |
130 |
24.8 |
1.2 |
74 |
|
8 |
6.2 |
6.2 |
6.2 |
136 |
26.0 |
1.3 |
78 |
|
10 |
12.4 |
6.2 |
9.3 |
130 |
24.1 |
1.2 |
72 |
|
12 |
12.4 |
6.2 |
9.3 |
136 |
25.3 |
1.3 |
76 |
|
14 |
12.4 |
12.4 |
12.4 |
143 |
26.1 |
1.3 |
78 |
|
16 |
6.2 |
12.4 |
9.3 |
155 |
29.1 |
1.5 |
87 |
|
18 |
18.6 |
18.6 |
18.6 |
149 |
26.1 |
1.3 |
78 |
|
20 |
12.4 |
12.4 |
12.4 |
161 |
29.7 |
1.5 |
89 |
|
22 |
18.6 |
24.8 |
21.7 |
161 |
27.9 |
1.4 |
84 |
|
24 |
18.6 |
24.8 |
21.7 |
167 |
29.1 |
1.5 |
87 |
|
26 |
24.8 |
24.8 |
24.8 |
161 |
27.2 |
1.4 |
82 |
|
28 |
18.6 |
24.8 |
21.7 |
167 |
29.1 |
1.5 |
87 |
|
30 |
18.6 |
24.8 |
21.7 |
161 |
27.9 |
1.4 |
84 |
|
32 |
18.6 |
24.8 |
21.7 |
167 |
29.1 |
1.5 |
87 |
|
34 |
24.8 |
24.8 |
24.8 |
161 |
27.2 |
1.4 |
82 |
|
36 |
18.6 |
24.8 |
21.7 |
167 |
29.1 |
1.5 |
87 |
|
38 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
|
40 |
24.8 |
24.8 |
24.8 |
161 |
27.2 |
1.4 |
82 |
|
42 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
|
44 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
|
46 |
24.8 |
24.8 |
24.8 |
161 |
27.2 |
1.4 |
82 |
|
48 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
90 |
|
50 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
90 |
|
52 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
|
54 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
90 |
|
56 |
24.8 |
24.8 |
24.8 |
174 |
29.8 |
1.5 |
90 |
|
58 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
|
60 |
24.8 |
24.8 |
24.8 |
167 |
28.4 |
1.4 |
85 |
Table 5.2.1/5: Percentage Biodegradation in Toxicity
days |
blank 1 |
blank 2 |
average |
toxicity |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.0 |
0 |
|
2 |
6.2 |
0 |
3.1 |
86.8 |
16.7 |
0.8 |
19 |
|
4 |
6.2 |
0 |
3.1 |
118 |
23.0 |
1.1 |
27 |
|
6 |
6.2 |
6.2 |
6.2 |
136 |
26.0 |
1.3 |
30 |
|
8 |
6.2 |
6.2 |
6.2 |
167 |
32.2 |
1.6 |
37 |
|
10 |
12.4 |
6.2 |
9.3 |
186 |
35.3 |
1.8 |
41 |
|
12 |
12.4 |
6.2 |
9.3 |
205 |
39.1 |
2.0 |
45 |
|
14 |
12.4 |
12.4 |
12.4 |
223 |
42.1 |
2.1 |
49 |
|
16 |
6.2 |
12.4 |
9.3 |
242 |
46.5 |
2.3 |
54 |
|
18 |
18.6 |
18.6 |
18.6 |
242 |
44.7 |
2.2 |
52 |
|
20 |
12.4 |
12.4 |
12.4 |
261 |
49.7 |
2.5 |
58 |
|
22 |
18.6 |
24.8 |
21.7 |
273 |
50.3 |
2.5 |
58 |
|
24 |
18.6 |
24.8 |
21.7 |
279 |
51.5 |
2.6 |
60 |
|
26 |
24.8 |
24.8 |
24.8 |
279 |
50.8 |
2.5 |
59 |
|
28 |
18.6 |
24.8 |
21.7 |
285 |
52.7 |
2.6 |
61 |
|
30 |
18.6 |
24.8 |
21.7 |
292 |
54.1 |
2.7 |
63 |
|
32 |
18.6 |
24.8 |
21.7 |
298 |
55.3 |
2.8 |
64 |
|
34 |
24.8 |
24.8 |
24.8 |
298 |
54.6 |
2.7 |
63 |
|
36 |
18.6 |
24.8 |
21.7 |
304 |
56.5 |
2.8 |
65 |
|
38 |
24.8 |
24.8 |
24.8 |
310 |
57.0 |
2.9 |
66 |
|
40 |
24.8 |
24.8 |
24.8 |
310 |
57.0 |
2.9 |
66 |
|
42 |
24.8 |
24.8 |
24.8 |
316 |
58.2 |
2.9 |
67 |
|
44 |
24.8 |
24.8 |
24.8 |
310 |
57.0 |
2.9 |
66 |
|
46 |
24.8 |
24.8 |
24.8 |
316 |
58.2 |
2.9 |
67 |
|
48 |
24.8 |
24.8 |
24.8 |
323 |
59.6 |
3.0 |
69 |
|
50 |
24.8 |
24.8 |
24.8 |
329 |
60.8 |
3.0 |
70 |
|
52 |
24.8 |
24.8 |
24.8 |
329 |
60.8 |
3.0 |
70 |
|
54 |
24.8 |
24.8 |
24.8 |
335 |
62.0 |
3.1 |
72 |
|
56 |
24.8 |
24.8 |
24.8 |
335 |
62.0 |
3.1 |
72 |
|
58 |
24.8 |
24.8 |
24.8 |
335 |
62.0 |
3.1 |
72 |
|
60 |
24.8 |
24.8 |
24.8 |
335 |
62.0 |
3.1 |
72 |
Table 5.2.1/6: Percentage Biodegradation in Abiotic Test
blank 1 |
blank 2 |
average |
abiotic |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
||
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.0 |
0 |
|
2 |
6.2 |
0 |
3.1 |
0 |
-0.6 |
0.0 |
-1 |
|
4 |
6.2 |
0 |
3.1 |
6.2 |
0.6 |
0.0 |
1 |
|
6 |
6.2 |
6.2 |
6.2 |
6.2 |
0.0 |
0.0 |
0 |
|
8 |
6.2 |
6.2 |
6.2 |
6.2 |
0.0 |
0.0 |
0 |
|
10 |
12.4 |
6.2 |
9.3 |
0 |
-1.9 |
-0.1 |
-3 |
|
12 |
12.4 |
6.2 |
9.3 |
0 |
-1.9 |
-0.1 |
-3 |
|
14 |
12.4 |
12.4 |
12.4 |
6.2 |
-1.2 |
-0.1 |
-2 |
|
16 |
6.2 |
12.4 |
9.3 |
6.2 |
-0.6 |
0.0 |
-1 |
|
18 |
18.6 |
18.6 |
18.6 |
0 |
-3.7 |
-0.2 |
-7 |
|
20 |
12.4 |
12.4 |
12.4 |
6.2 |
-1.2 |
-0.1 |
-2 |
|
22 |
18.6 |
24.8 |
21.7 |
12.4 |
-1.9 |
-0.1 |
-3 |
|
24 |
18.6 |
24.8 |
21.7 |
12.4 |
-1.9 |
-0.1 |
-3 |
|
26 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
28 |
18.6 |
24.8 |
21.7 |
6.2 |
-3.1 |
-0.1 |
-5 |
|
30 |
18.6 |
24.8 |
21.7 |
6.2 |
-3.1 |
-0.1 |
-5 |
|
32 |
18.6 |
24.8 |
21.7 |
6.2 |
-3.1 |
-0.1 |
-5 |
|
34 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
36 |
18.6 |
24.8 |
21.7 |
6.2 |
-3.1 |
-0.1 |
-5 |
|
38 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
40 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
42 |
24.8 |
24.8 |
24.8 |
12.4 |
-2.5 |
-0.1 |
-4 |
|
44 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
46 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
48 |
24.8 |
24.8 |
24.8 |
12.4 |
-2.5 |
-0.1 |
-4 |
|
50 |
24.8 |
24.8 |
24.8 |
12.4 |
-2.5 |
-0.1 |
-4 |
|
52 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
54 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
56 |
24.8 |
24.8 |
24.8 |
12.4 |
-2.5 |
-0.1 |
-4 |
|
58 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
|
60 |
24.8 |
24.8 |
24.8 |
6.2 |
-3.7 |
-0.2 |
-7 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test material (test1) attained 45% degradation after 28 days.
The test material (test 2) attained 61% degradation after 28 days and didn’t satisfy the 10-day window validation criterion.
Therefore the test material cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline N° 301F.
In the prolonged Manometric Respirometry test, the test material (test1and2) attained respectively 62% and 72% at day 60, demonstrating that the test substance is not persistent. Moreover, the biodegradation percentage reached at day 60 demonstrates that the test material is ultimately (completely) biodegradable according to ECHA (European Chemicals Agency, ECHA guidance chR7b, p179&198, ”Enhanced biodegradation test”). - Executive summary:
The study was performed to assess the ready biodegradability of the test material in an aerobic aqueous media. The method followed that described in the OECD Guideline for testing Chemicals (1992) N°301 F, “Ready Biodegradability; Manometric Respirometry Test”. The test material, at a concentration of 102.5mg/L in test 1, at a concentration of 97.5 mg/L in test 2 was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in diffused light at 22°C ± 1°C for 60 days.
The test material (test1) attained 45% degradation after 28 days. The test material (test 2) attained 61% degradation after 28 days and didn’t satisfy the 10-day window validation criterion. Therefore the test material cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline N° 301F.
In the prolonged Manometric Respirometry test, the test material (test1and2) attained respectively 62% and 72%at day 60, demonstrating that the test substance is not persistent. Moreover, the biodegradation percentage reached at day 60 demonstrates that the test material is ultimately (completely) biodegradable according toECHA (European Chemicals Agency, ECHA guidance chR7b, p179&198, ”Enhanced biodegradation test”).
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