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EC number: 943-330-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Sept - 20 Oct 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions: Analytical purity not stated.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- The Department of Health of the Government of the United Kingdom
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was collected from the aeration stage of the Severn Trent Plc sewage treatment plant at Belper, Derbyshire, which treats predominantly domestic sewage. (date of collection: 22 Sept 1992)
- Pretreatment: The activated sludge was homogenized for 10 min. The solids were then removed by centrifuging and the supernatant was taken for testing. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Solution 1: FeCl3x6H2O = 0.25 g/L; Solution 2: MgSO4x7H2O = 22.5 g/L; Solution 3: CaCl2 = 27.5 g/L; Solution 4: (NH4)2SO4 = 40 g/L; Solution 5: KH2PO4 = 8.5 g/L, K2HPO4 = 27.75 g/L, Na2HPO4x7H2O = 33.4 g/L, NH4Cl = 1.7 g/L
4 mL Solution 1, 1 mL of each Solution 2, 3 and 4 and 2 mL Solution 5 were added to 1 L of purified water
- Test temperature: 21 ± 1 °C
- pH: day 27: 10 mg/L = 5.4; 20 mg/L = 5.6; control = 5.3; standard = 5.4
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5 L glass culture vessel containing 3 L
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The culture vessels were sealed and CO2-free air bubbled through the solution at a rate of ~ 2 bubbles per second and stirred continuously by magnetic stirrers.
- Measuring equipment: Ionics 555 TOC analyser
- Details of trap for CO2 and volatile organics if used: CO2 was absorbed using 2 500 mL Dreschel bottles filled with 350 mL 0.05 M NaOH
SAMPLING
- Sampling frequency: Samples (2 mL, in triplicate) were taken from the first absorber vessel on days 0, 1, 2, 3, 6, 8, 10, 14, 16, 21, 23, 27 and 28. The second absorber vessel was sampled on days 0 and 28.
- Sampling method: On day 27 the pH of each test vessel was measured and 1 mL of concentrated HCl added to drive of inorganic carbonate. The vessels were aerated overnight and the final samples were taken from both absorber vessels on day 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 (with and without standard) - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 74
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg/L test substance concentration
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg/L test substance concentration
- Details on results:
- The test substance reached biodegradation values of 74 (10 mg/L) and 82% (20 mg/L) after 28 days test duration. Furthermore,
degradation over 60% took place within the 10d-window period. Therefore, the test substance can be considered as readily biodegradable. - Results with reference substance:
- Sodium acetate reached a biodegradation value of 86% at the end of the test. Besides, degradation over 60% occurred within 14 days (79%).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- 1. SOFTWARE
Vega version 1.1.4
2. MODEL (incl. version number)
Ready Biodegradability Model (IRFMN) v. 1.0.9
3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
See “Test material information”
4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
See attached information on the model provided by the developer. Further information on the OECD criteria as outlined by the applicant is provided below under "Any other information of materials and methods incl. tables"
5. APPLICABILITY DOMAIN
See attached information and information as provided in "Any other information on results incl. tables".
6. ADEQUACY OF THE RESULT
See assessment of adequacy as outlined in the "Overall remarks, attachments" section. - Principles of method if other than guideline:
- - Software tool(s) used including version: Vega v1.1.4
- Model(s) used: Ready biodegradability model (version 1.0.9)
Full reference and details of the used formulas can be found in:
Meylan W.M., Howard P.H., Boethling R.S. et al. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. 1999, Environ. Toxicol. Chem. 18(4): 664-672
- Model description: see field 'Justification for non-standard information', 'Attached justification' and 'any other information on Material and methods'
- Justification of QSAR prediction: see field 'Justification for type of information', 'Attached justification' and/or 'overall remarks' - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Remarks on result:
- other: The prediction for the substance component is Readily Biodegradable.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- 1. SOFTWARE
Vega version 1.1.4
2. MODEL (incl. version number)
Ready Biodegradability Model (IRFMN) v. 1.0.9
3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
See “Test material information”
4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
See attached information on the model provided by the developer. Further information on the OECD criteria as outlined by the applicant is provided below under "Any other information of materials and methods incl. tables"
5. APPLICABILITY DOMAIN
See attached information and information as provided in "Any other information on results incl. tables".
6. ADEQUACY OF THE RESULT
See assessment of adequacy as outlined in the "Overall remarks, attachments" section. - Principles of method if other than guideline:
- - Software tool(s) used including version: Vega v1.1.4
- Model(s) used: Ready biodegradability model (version 1.0.9)
Full reference and details of the used formulas can be found in:
Meylan W.M., Howard P.H., Boethling R.S. et al. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. 1999, Environ. Toxicol. Chem. 18(4): 664-672
- Model description: see field 'Justification for non-standard information', 'Attached justification' and 'any other information on Material and methods'
- Justification of QSAR prediction: see field 'Justification for type of information', 'Attached justification' and/or 'overall remarks' - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Remarks on result:
- other: The prediction for the substance component is Readily Biodegradable.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Remarks:
- Summary of available data used for the endpoint assessment of the target substance.
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue justification attached to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 74
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg/L test substance concentration
- Remarks:
- Source, RA-A, CAS 141-17-3
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg/L test substance concentration
- Remarks:
- Source, RA-A, CAS 141-17-3
Referenceopen allclose all
For detailed information on the results please refer to the attached report.
For detailed information on the results please refer to the attached report.
Description of key information
Readily biodegradable: 74 - 82% in 28 days (OECD 301B); read across;
QSAR prediction: readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Experimental data on the biodegradability of the target substance Reaction mass of bis[2-[2-(2-butoxyethoxy)ethoxy]ethyl] adipate and [2-[2-(2-butoxyethoxy)ethoxy]ethyl](3,6,9,12-tetraoxahexadecyl)adipate (EC 943-330-9) are not available. The assessment was therefore based on studies conducted with the analogue substance Bis(2-(2-butoxyethoxy)ethyl) adipate (CAS 141-17-3) and on QSAR calculations performed with the main substance components. The read across approach is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Please see the analogue justification attached to IUCLID section 13 for a detailed justification.
The biodegradability of the source substance Bis(2-(2-butoxyethoxy)ethyl) adipate (CAS 141-17-3) was tested according to OECD 301B under GLP conditions using non-adapted activated sludge as inoculum. The degradation rate was tested with two different test substance concentrations i.e. 10 mg and 20 mg test material/L. After 28 days a mean degradation rate of 74 % was observed at a test substance concentration of 10 mg/L). The observed degradation rate at 20 mg/L was 82% after 28 days. The 10-day-window was met and the substance was considered as readily biodegradable according to OECD criteria.
The read across approach is supported by QSAR calculations performed with two main substance components of the target substance. Ready biodegradability was predicted for both substance components (VEGA 1.1.4, Ready biodegradability model v1.0.9).
Based on the results for the source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5), as well as on the results of the QSAR calculation, Reaction mass of bis[2-[2-(2-butoxyethoxy)ethoxy]ethyl] adipate and [2-[2-(2-butoxyethoxy)ethoxy]ethyl](3,6,9,12-tetraoxahexadecyl)adipate (EC 943-330-9) is considered as readily biodegradable.
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