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EC number: 219-948-3 | CAS number: 2580-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 2 September 1997 and 16 September 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: "Standards for Mutagenicity Test using Microorganisms" of "Occupational Safety and Health Law"
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(2-hydroxyethyl) sulphone
- EC Number:
- 219-948-3
- EC Name:
- Bis(2-hydroxyethyl) sulphone
- Cas Number:
- 2580-77-0
- Molecular formula:
- C4H10O4S
- IUPAC Name:
- 2-(2-hydroxyethanesulfonyl)ethan-1-ol
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Test substance: BS-1
Sample nurnber: 97-180
Concentration: 65 percent aqueous solution
Method
- Target gene:
- histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- The highest dose of the test solution was 10μl because the concentration of test substance was 65%.
Solutions of five different concentration, 0. 391, 0. 156, 0. 6 25, 2. 50 and 10. 0 μl per per plate were used for dose range finding test.
For main test, concentration of the test solution was prepared to be 0. 6 25, 1.25, 2.50, 5.00 and 10.0 μl per plate. - Vehicle / solvent:
- sterilized pure water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- sterilized pure water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- dissolved in DMSO. 0.01 and 0.1µg/plate for TA100 and TA98
- Positive control substance:
- other: 2- ( 2-Furyl)-3- (5-nitro- 2-furyl)acrylamide (AF2)
- Remarks:
- without S9 mix
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- sterilized pure water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- dissolved in DMSO. 5 µg/plate for TA100 and TA98
- Positive control substance:
- other: Benzo[a]pyrene (BP)
- Remarks:
- with S9 mix
- Details on test system and experimental conditions:
- Control test
For the solvent control test, three plates were used.
Meanwhile, two plates were used for positive control test.
Every positive control was dissolved in DMSO and stored in a freezer at - 20 °C.
For the test substance two plates of each dose were used in every test.
Test method
Test solution (0.1 ml) was mixed with 0.1 M sodium phosphate buffer (pH 7.4, 0.5 ml) and the overnight culture of tester strain (0.1 ml) and then the mixture was pre-incubated at
37 °C for 20 minutes with gentle shaking. For metabolic activation, S9 Mix (0.5 ml) was mixed instead of 0.1 M sodium phosphate buffer. After pre-incubation, melted soft agar ( 2 ml)
was added and the resulting mixture was poured onto minimal glucose agar plate. The soft agar contained 0.5 mM D-biotin and 0.5 mM L-histidine.
After incubation for 2 days, revertant colonies were counted and average number of colonies in each dose and control were obtained.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Dose range finding test:
The number of revertant colonies did not increase twice or more over that in negative control in test strains both with and without S9Mix.
Main test:
The number of revertant colonies did not increase twice or more over that in negative control in test strains both with and without S9Mix.
Any other information on results incl. tables
Results from the main test are presented in the table below
Test period |
97/9/11~97/9/16 |
||||
With (+) or without (-) S9 mix |
Dose µg/plate |
Number of revertant colonies /plate |
|||
Base-pair substitution type |
Frameshift type |
||||
TA100 |
TA98 |
||||
S9 Mix (-) |
Solvent control |
127 |
|
18 |
|
167 |
|
28 |
|
||
140 |
(145) |
23 |
(23) |
||
1 0.625 |
136 |
|
25 |
|
|
145 |
(141) |
14 |
(20) |
||
2 1.25 |
155 |
|
19 |
|
|
127 |
(141) |
23 |
(21) |
||
3 2.5 |
140 |
|
18 |
|
|
140 |
(140) |
26 |
(22) |
||
4 5 |
144 |
|
14 |
|
|
128 |
(136) |
23 |
(19) |
||
5 10 |
137 |
|
21 |
|
|
142 |
(140) |
23 |
(22) |
||
S9 Mix (+) |
Solvent control |
139 |
|
38 |
|
116 |
|
48 |
|
||
127 |
(127) |
40 |
(42) |
||
1 0.625 |
112 |
|
39 |
|
|
161 |
(137) |
43 |
(41) |
||
2 1.25 |
122 |
|
40 |
|
|
145 |
(134) |
40 |
(40) |
||
3 2.5 |
155 |
|
37 |
|
|
130 |
(143) |
40 |
(39) |
||
4 5 |
136 |
|
28 |
|
|
148 |
(142) |
44 |
(36) |
||
5 10 |
133 |
|
27 |
|
|
155 |
(144) |
40 |
(34) |
||
Positive control without S9 mix |
Chemical |
AF2 |
AF2 |
||
Dose µg/plate |
0.01 |
0.1 |
|||
Revertants /plate |
1166 1240 |
(1203) |
164 140 |
(152) |
|
Positive control with S9 mix |
Chemical |
BP |
BP |
||
Dose µg/plate |
5 |
5 |
|||
Revertants /plate |
1122 1036 |
(1079) |
293 367 |
(330) |
The figures in parentheses show the mean of each plate.
Positive controls
AF2: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide
BP: Benzo[a]pyrene
Applicant's summary and conclusion
- Conclusions:
- The number of revertant colonies of BS-1 did not show any tendency to increase twice or more over that in negative control
for all strains. It was confirmed that the test was appropriately performed because the negative control and the
positive control induced the reasonable increase in the number of revertant colonies.
Based on the above results, it is concluded that BS-1 was non-mutagenic in this test system. - Executive summary:
BS-1 was tested for mutagenicity using Salmonella typhimurium (TA100 and TA98) as indicator strains and the liver microsome fraction of rats for metabolic activation system (S9 Mix).
The test was carried out according to "Standards for Mutagenicity Test using Microorganisms" of "Occupational Safety and Health Law" and "GLP Standards Applied to Industrial Chemicals" of "Law Concerning the Examination and Regulation of Manufacture, etc. , of Chemical Substance".
The test substance was judged non-mutagenic in this test system.
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