Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-714-1 | CAS number: 16669-27-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- For "read across justification" please refer to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- For "read across justification" please refer to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Details on test conditions:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The physico-chemical oxygen uptake was determined by a test mixture containing the highest concentrations of test substance, synthetic sewage and distilled water but no activated sludge. The measured data of the test mixtures containing test substance and inoculum were corrected by this value.
TEST CONCENTRATIONS
As the solubility of 2.4 g test substance/l could not be obtained, the test material was added directly to the test mixtures.
Five concentrations of the test material (1, 10, 100, 1000, 10000 mg/l) were tested. As the solubility of the substance was far exceeded and no physical inhibitory effects (e.g. agglomeration of activated sludge) were observed, no further tests were performed.- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Endpoint:
- toxicity to microorganisms
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- For "read across justification" please refer to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 16 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 936 mg/L
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- For "read across justification" please refer to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 5 d
- Dose descriptor:
- EC0
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Study period:
- 1989-07-18 to 1989-07-20
- Justification for type of information:
- For "read across justification" please refer to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- yes
- Key result
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Referenceopen allclose all
Description of key information
The EC50 of the substance is determined to be > 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
Discussion
No experimental data on toxicity to microorganisms are available for the substance (CAS 16669-27-5) itself. Therefore, the ecotoxicological potential was assessed based on data available for the close structural analogues isotridecyl methacrylate (CAS 94247-059), octadecyl methacrylate (CAS 32360-05-7), methacrylic acid ester 13.6 (CAS 90552-04-8) and dodecyl methacrylate (CAS 142-90-5). Several tests with activated sludge are available, all of them valid without or with minor restrictions only. In accordance with Annex XI section 1.2 of Regulation (EC) No 1907/2006 (REACH), the information was evaluated in a weight of evidence approach.
In a static Activated Sludge Respiration Inhibition Test in compliance with GLP criteria and according to OECD Guideline 209, the close structural analogue isotridecyl methacrylate (CAS 94247-059) was tested over an duration of 180.0 min for toxicity to bacteria relevant for the biodegradation of the substance (Evonik Röhm GmbH, 1989). Mixed liquor from activated sludge of a predominantly domestic sewage treatment plant was obtained and aerated during transportation to the laboratory. Two samples of 10 ml were weighed and dried at 105 °C. The amount of solids was calculated and the wet sludge was suspended with isotonic solution to yield a concentration of 3 g suspended solids per litre. Subsequently, the activated sludge was amended with 50 ml synthetic sewage per litre and aerated at 20 °C + 2 °C overnight. Before use the amount of solids was determined and adjusted to 3 g/L. Aditionally, the pH-value was controlled. An adjustment was not necessary as the pH was in the accepted range of 6.0 – 8.0. The test concentrations were 1, 10, 100, 1000 and 10000 mg/L in nominal. As the solubility of the substance was far exceeded and no physical inhibitory effects (e.g. agglomeration of activated sludge) were observed, no further tests were performed. In addition, a positive control with the reference substance 3,5-Dichlorophenol was performed. In result, at concentrations up to 10000 mg/L, which are far exceeding the water solubility of the substance, no inhibition of the oxygen consumption of the bacteria was observed within 180 min. The EC50 (3 hours) of 3,5-dichlorophenol was in the accepted range of 5 to 30 mg/L. Therefore, it can be concluded that the test is valid and the close structural analogue is non-toxic to bacteria.
In a static Activated Sludge Respiration Inhibition Test according to EU Method C.11 and in compliance with GLP criteria, the close structural analogue octadecyl methacrylate (CAS 32360-05-7) was tested over an duration of 180.0 min (test temperature: 20 °C ± 2 °C; pH 6.0 - 8.0) for toxicity to bacteria relevant for the biodegradation of the substance (Evonik RohMax GmbH, UNTER 88-011, 1988). A sample of mixed liquor from a predominantly domestic sewage plant was obtained. Five concentrations of the test material (1, 10, 100, 1000, 10000 mg/L) were tested. In addition, a positive control with the reference substance 3,5-Dichlorophenol was performed. As the solubility of octadecyl methacrylate was far exceeded and no physical inhibitory effects (e.g. agglomeration of activated sludge) were observed, no further tests were performed. In the first run of the experiment, the respiration rate after 180 min contact time could not be determined. the oxygen concentration in the measuring cell decreased rapidly. As consequence of technical reasons, the oxygen uptake after 30 min could not be determined in the second test run. In result, at concentrations up to 10000 mg/L, which are far exceeding the water solubility of the the structural analogue, no inhibition of the oxygen consumption of the bacteria was observed within 180 min. Therefore, it can be concluded that this close structural analogue is non-toxic to bacteria. The 3-hours EC50 of 3,5-dichlorophenol was in the accepted range of 5 to 30 mg/l. The test is regarded as valid.
In an Activated Sludge Respiration Inhibition Test in compliance with GLP criteria and according to OECD Guideline 209, the close structural analogue methacrylic acid methyl ester 13,6 (CAS 90552-04-8) was tested over an duration of 180.0 min for toxicity to bacteria relevant for the biodegradation of the substance (Evonik RohMax Additives GmbH, UNTER 88-012, 1988). The EC10 (3 h) for the inhibition of biodegradation was > 10000 mg/L. Therefore, it can be concluded that this close structural analogue is non-toxic to bacteria.
In a Growth Inhibition Test according to DEV L 8 ("Deutsches Einheitsverfahren", German standard method), Pseudomona gluorescens was exposed to the close structural analogue octadecyl methacrylate (CAS 32360-05-7). The principle of the test is that dissolved toxic substances inhibit the ability of the bacterium Pseudomonas gluorescens to produce organic acids from glucose. As a result, the EC0 (16 h) was > 936 mg/L. It can be concluded, that this close structural analogue is non-toxic to bacteria.
The toxicity to bacteria relevant for the biodegradation of the close structural analogue methacrylic acid methyl ester 13,6 (CAS 90552-04-8) was assessed in a static Activated Sludge Respiration Inhibition Test according to OECD Guideline 209 (Evonik Röhm GmbH, UNTER 88-032, 1989). Deviating from the guideline, the contact time was about 5 days (according to Offhaus). Due to low solubility, the close structural analogue was tested emulsified at concentrations of 100, 200 and 500 mg/L. In result, no inhibition of respirometric activity in any of the tested concentrations was observed. The 5-d EC0 (bacteria) was determined to be > 500 mg/L in emulsion according to OECD guideline 209.
Overall conclusion
The close structural analogues tested had an moiety chain length of C12–C22. No toxicity to bacteria was observed, the EC0 / EC50 values were, depending on the test conditions, between > 500 and > 10000 mg/L. Thus, concentrations of the test substances were several orders of magnitude above the limit of solubility. There is, however, no indication that the undissolved test substances interfered with bacterial respiration or the functioning of the test system. In conclusion, no inhibition of biodegradability in STPs or in the environment is expected.
Based on the most reliable information (Evonik Röhm GmbH, UNTER 89 -013, 1989), the EC 50 of docosyl methacrylate (CAS 16669 -27 -5) is considered to be > 10000 mg/L. This value is supported by two other studies (Evonik RohMax GmbH, UNTER 88-011, 1988; Evonik RohMax Additives GmbH, UNTER 88-012) since they had the same result (EC50 > 10000 mg/L). In two further studies (Evonik RohMax GmbH, UNTER 88-030, 1988; Evonik Röhm GmbH, UNTER 88-032, 1989) the estimated EC50 values were above the highest concentration tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.