Biphenyl-2-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9’-spirobifluoren-2-yl)-amine

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug 21, 2014 - Nov 17, 2014

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no OECD GLP

Justification for type of information:

This study was performed according OECD TG 207. The quality system applied was not OECD GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

no

Remarks:

no OECD GLP

Test material

Test material information

Constituent 1

Reference substance name:

N-{[1,1'-biphenyl]-2-yl}-N-(9,9-dimethyl-9H-fluoren-2-yl)-9,9'-spirobi[fluoren]-2-amine

EC Number:

700-681-0

Cas Number:

1364603-07-5

Molecular formula:

C52H37N

IUPAC Name:

N-{[1,1'-biphenyl]-2-yl}-N-(9,9-dimethyl-9H-fluoren-2-yl)-9,9'-spirobi[fluoren]-2-amine

Test material form:

solid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: OS14001822
- Expiration date of the lot/batch: 2019-04-30

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

Acetone

Details on preparation and application of test substrate:

A defined artificial soil of the following composition was used for this assay.

Sphagnum peat 222g
Kaolinite clay: 444 g
Silica sand(screened with 0.2mm sieve) 1556 g
Water: 778 mL


Test subsance amount was calculated based on dry weight

Test organisms

Test organisms (species):

Eisenia fetida

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia fetida
- Source: Jia Liming Earthworm Cultivation Ltd. Tianjin, China
- Breeding generation: EFA000-20140715-02
- Age at test initiation (mean and range, SD): < 2 month
- Weight at test initiation (mean and range, SD): 455 mg

ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): no feeding
- Health during acclimation (any mortality observed): no mortality, no other findings

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

19.2 - 21.2 °C

pH:

5.78 - 5.84

Moisture:

35 %

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4



OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 621 - 705 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 0 and 14

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
1000 mg/kg dw

Nominal and measured concentrations:

1000 mg/kg dw

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LD50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The mortalities of the blank control group, the solvent control group and the treatment group on day 14 were 0%.

Results with reference substance (positive control):

LD50 at about 15 mg/kg soil dw

Reported statistics and error estimates:

Standard statistical methods have been applied for data processing.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.