Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 212-593-5 | CAS number: 830-03-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Acetic acid, 4-nitrophenyl ester
- IUPAC Name:
- Acetic acid, 4-nitrophenyl ester
- Reference substance name:
- 4-nitrophenyl acetate
- EC Number:
- 212-593-5
- EC Name:
- 4-nitrophenyl acetate
- Cas Number:
- 830-03-5
- Molecular formula:
- C8H7NO4
- IUPAC Name:
- 4-nitrophenyl acetate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 4-Nitrophenyl Acetate
- Physical state: solid
- Analytical purity: 95 % w/w
- Impurities (identity and concentrations):
Phenol <5.0 % (w/w)
4-nitrophenol <5.0 % (w/w)
Acetic acid <1.0 % (w/w)
Water <5.0 % (w/w)
- Lot/batch No.: 201409
- Expiration date of the lot/batch: unlisted
- Storage condition of test material: During the study the test substance was stored in closed
container in the dark at laboratory temperature.
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- Preparation of inoculum for exposure: The fresh collected waste water was filtered through paper filter. COD was determined and waste water was aerated till using.
Initial test substance concentration
- Initial conc.:
- 3.39 mg/L
- Based on:
- COD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Inoculated medium
The mineral medium was strongly aerated for 20 minute and let standing for 20 hours at the test temperature. The volume of waste water for the inoculation of mineral medium was chosen 2.1 mL per 1 L of medium (COD of waste water: 61 mg·L-1).
30 L aerated mineral medium was prepared for the test and before the beginning of the test it was inoculated with 63 mL of modified waste water. The pH value of medium: 7.4.
Test substance
The stock solution of test substance was prepared in concentration 0.1010 g.L-1 of water.
From this solution 140.4 mL (23.4 mL.L-1) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L.
The solution prepared in this way contained 2.36 mg·L-1 of test substance, and had COD 3.39 mg.L-1. The pH value of solution: 7.4.
Reference substance
The stock solution of sodium benzoate was prepared in concentration 1.0000 g·L-1of water. From this solution the 12.6 mL (2.1 mL·L-1) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L.
The solution prepared in this way contained 2.10 mg·L-1 of sodium benzoate, and had COD 3.45 mg·L-1. The pH value of solution: 7.4.
Toxicity test
The solution was prepared by dosing 93.6 mL of stock solution of test substance and 8.4 mL of stock solution of reference substance into inoculated medium and completed with this medium to the volume of 4 L.
The mixture prepared in this way contained 2.36 mg·L-1 of test substance and 2.10 mg.L-1 of sodium benzoate and had COD 6.84 mg·L-1.
- Test temperature: 20 +- 1.0°C
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- 2 x 9 bottles with test substance and inoculated medium
- 2 x 9 bottles with reference substance and inoculated medium for the check of inoculum activity
- 9 bottles with test substance and inoculated medium for the determination of oxidized nitrogen forms
- 2 x 5 bottles with test and reference substance mixture and inoculated medium for the toxicity test
- 2 x 9 bottles with inoculated medium only for the blank determination
- Measuring equipment:
pH meter WTW Inolab pH 730
- analytical balance XS 105 DU (Mettler Toledo)
- UV/VIS spectrophotometer Helios Alpha
- equipment for the determination of nitrite
- equipment for the determination of nitrate
- volumetric flasks, flasks, beakers, pipettes and further common laboratory equipment
SAMPLING
- Sampling frequency: At the zero-time and at the 3rd, 7th, 10th, 14th, 17th, 21st, 24th and 28th day of test the appropriate bottles from each series were taken off
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70.4
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- BOD5
- Value:
- 0.238 other: mg/mg
- Parameter:
- COD
- Value:
- 1.436 other: mg/mg
- Results with reference substance:
- COD of the reference substance: 1.641 mg.mg-1
Any other information on results incl. tables
The oxygen consumption and percentage of the biodegradation of the test substance for each test interval and each bottle were calculated. In time intervals when the increase of oxidized nitrogen forms was detected, the correction of oxygen consumption for nitrification was carried out. The evaluation of the reference substance and of the test and reference substance mixture was done by the same way. All the calculations were related to the initial values of COD of the test and reference substance. Formulae for the calculations are given in tables 4 and 5. The results of the test were plotted into a graph from which the degree of degradability in percentage in each time interval can be deduced.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In this 28-day study of ready biological degradability the degradation of 70.4 % of the test substance, 4-Nitrophenyl Acetate, was attained in the end of study
- Executive summary:
The test substance, 4-Nitrophenyl Acetate, was tested for the ready biological degradability in Closed Bottle Test. Test performance The test was performed according to: Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008. The results of biological degradation are related to experimentally determined COD values of test and reference substance at the beginning of the test. The test substance was sufficiently soluble in used mineral medium so the dosage from the stock solution was carried out. Sodium benzoate was used as the reference substance. The dosage was carried out from the stock solution. COD of the test substance in medium at the beginning of the main test: 3.39 mg·L-1 COD of reference substance in medium at the beginning of the main test: 3.45 mg·L-1 In parallel to the main test the toxicity test was performed. Based on the test substance contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out. The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7-8 at the beginning of the test. Validity of the test The prescribed validity criteria in the test were fulfilled. The test substance was not inhibiting for the used inoculum. Since all criteria of acceptability were met, this study is considered to be valid. Test results In this 28-day study of ready biological degradability the degradation of 70.4 % of the test substance, 4-Nitrophenyl Acetate, was attained in the end of study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.