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EC number: 249-854-8 | CAS number: 29797-40-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Dichloromethylbenzene
- EC Number:
- 249-854-8
- EC Name:
- Dichloromethylbenzene
- Cas Number:
- 29797-40-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- (dichloromethyl)benzene
- Details on test material:
- content: 99.02% (analytical result)
composition: 44.8 % 2.5-Dichlorotoluene
25.9 % 2.4-Dichlorotoluene
12.6 % 3.4-Dichlorotoluene
9.2 % 2.3-Dichlorotoluene
6.6 % 2.6-Dichlorotoluene
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- single treatment
- Frequency of treatment:
- single treatment
- Post exposure period:
- Time of sacrifice:
negative control: 24 hrs,
test substance: 16, 24, 48 hrs,
positive control cyclophosphamide: 24 hrs
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1500 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- not specified
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Summary of results of Micronucleus test with Dichlorotoluene (mixture of isomers) (after acute intraperitoneal treatment with 1500 mg/kg body weight:
micronucleated cells per 1000 | ||||
experimental groups | number of evaluated polychromatic erythrocytes | number of normochromatic erythrocytes per 1000 polychromatic erythrocytes | normochromatic erythrocytes | polychromatic erythrocytes |
negative control | 10.000 | 1138 + 460 | 2.0 + 2.5 | 2.0 + 2.2 |
test substance 16 hrs | 10.000 | 977 + 232 | 1.9 + 1.3 | 1.7 + 1.5 |
test substance 24 hrs | 10.000 | 1149 + 243 | 1.9 + 1.2 | 2.0 + 1.8 |
test substance 48 hrs | 10.000 | 870 + 242 | 2.2 + 2.0 | 2.3 + 1.8 |
positive control CP 20 mg/kg | 10.000 | 728 + 227 | 1.6 + 1.4 | 19.1* + 10.4 |
*P< 0.01 in non-parametric Wilcoxon ranking test
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
- Executive summary:
This micronucleus test was conducted to investigate the test substance dichlorotoluene (mixture of isomers) in male and female mice for a possible clastogenic effect on the chromosomes of bone marrow erythroblasts. The known clastogen and cytostatic agent, cyclophosphamide, served as control.
The treated animals received a single intraperitoneal administration of either the test substance or cyclophosphamide. The femoral marrow of groups treated with the test substance was prepared 16, 24 and 48 hours after administration. All negative and positive control animals were sacrificed after 24 hours. The doses of the test substance and the positive control, cyclphosphamide, were 1500 and 20 mg/kg body weight, respectively.
The animals treated with the test substance showed symptoms of toxicity (apathy, stretching of body, roughed fur, staggering gait, spasm and difficulty in breathing) after administration. However, all animals survived untill the end of the test.
There was no altered ratio between polychromatic and normochromatic erythrocytes.
Cyclophosphamide, the positive control, had a clear clastogenic effect, as is shown by the biologically relevant increase in polychromatic erythrocytes with micronuclei. The ratio of polychromatic to normochromatic erythrocytes was not altered.
No indications of a clastogenic effect of the test substance dichlorotoluene (mixture of isomers) were found after a single intraperitoneal treatment with 1500 mg/kg.
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