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EC number: 265-165-5 | CAS number: 64742-61-6 A complex combination of hydrocarbons obtained from a petroleum fraction by solvent crystallization (solvent dewaxing) or as a distillation fraction from a very waxy crude. It consists predominantly of saturated straight and branched chain hydrocarbons having carbon numbers predominantly greater than C20.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1985-08-20 to 1986-02-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it was conducted according to or similar to guideline study OECD TG 402.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- Four instead of 5 animals per sex used, animals not transferred to clean cages every 2 weeks, temperature ranged from 21 to 24 degrees celsius instead of 21 +/- 2 degrees Celsius
- GLP compliance:
- yes
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- 64741-50-0
- Cas Number:
- 64741-50-0
- IUPAC Name:
- 64741-50-0
- Reference substance name:
- Unrefined light paraffinic distillate
- IUPAC Name:
- Unrefined light paraffinic distillate
- Test material form:
- other: oily liquid
- Details on test material:
- - Name of test material (as cited in study report): Light Paraffinic Distillate
- Test substance: Unrefined light paraffinic distillate
- Molecular weight (if other than submission substance): 296
- Physical state: Liquid (light amber)
- CAS number: 64741-50-0
- Viscosity, cSt: 2.67 at 100°C
- API Gravity: 31.5
- Flash Point: (°F) 372
- Sulfur, Wt %: 0.2
- Nitrogen ppm: 256
- Distillation ASTM D 86 equivalent (°F) range: 601-803 (10-95%)
- Initial Boiling Point (°F): 579
- Composition of test material, Wt. %:
Olefins: 39.7
Naphthenes 21.7
- PONA % by MS: 37
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: Approximately 14 weeks
- Weight at study initiation: 2561 to 3192 grams
- Housing:
- Diet (e.g. ad libitum): Ad libitum, Purina Certified Rabbit Chow F5322
- Water (e.g. ad libitum): Ad libitum
- Acclimation period: Not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 to 24
- Humidity (%): 56 to 70
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure:
- % coverage: 10 % of total body area was shaved
- Type of wrap if used: eight-ply gauze bandage overwrapped with saran wrap and elastoplast tape.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): None
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 4
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observed for clinical signs and mortality hourly for the first 6 hours after dosing, then daily for dermal irritation and twice daily for clinical signs and mortality for a period of 14 days
- Necropsy of survivors performed: Yes
- Other examinations performed: Clinical signs, body weight, histopathology - Statistics:
- No data reported.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- Number dead/Number dosed
Females (intact): 0/2
Females (abraded): 0/2
Males (intact): 0/2
Females (abraded): 0/2 - Clinical signs:
- other: With the exception of dermal irritation, the only clinical sign observed was soft stool on study day 4 for one male and three female animals.
- Gross pathology:
- Necropsy Findings: No visible lesions on 2 males and 4 females. Dry, crusty, tan material covering skin of test site on 2 males.
- Other findings:
- Body weights:
Average body weights (g)
Male (intact):
initial - 2603
day 7 - 2635
terminal - 2773
Male (abraded):
initial - 2697
day 7 - 2651
terminal - 2627
Female (intact):
initial - 2978
day 7 - 2892
terminal - 3171
Female (abraded):
initial - 3022
day 7 - 2985
terminal - 3246
Any other information on results incl. tables
Acute Dermal Toxicity
Summary of Clinical Signs
(Number of Animals Affected)
|
Hours |
Days |
||||||||||||||||||
Observation |
1 |
2 |
3 |
4 |
5 |
6 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
2.0 g/kg - Males |
||||||||||||||||||||
Appeared |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
3 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
Soft stool |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2.0 g/kg – Females |
||||||||||||||||||||
Appeared |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
1 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
Soft stool |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The dermal LD50 was determined to be greater than 2000 mg/kg of body weight for both males and females.
- Executive summary:
Read across justification
No acute dermal toxicity studies have been reported for slack waxes (carcinogenic or unknown feed-stock), but a study has been reported for unrefined / acid treated lubricant base oils, materials similar to the oil entrained in slack waxes (carcinogenic or unknown feed-stock).
In an acute dermal toxicity study, groups of 14 week old New Zealand White rabbits (4 male/4 female) were dermally exposed to light paraffinic distillate for 24 hours to 10% of body surface area at a single dose of 2000 mg/kg bw. Animals then were observed for 14 days.
With the exception of dermal irritation, the only clinical sign observed was soft stool on study day 4 for one male and three female animals. Dermal irritation observed during the study ranged from slight to severe for erythema and oedema, from slight to marked for fissuring, and from slight to moderate for atonia and desquamation. Slight coriaceousness was also observed. One male animal exhibited a body weight loss at days 7 and 14 weighings. This animal had an abraded test site. The dermal LD50 was determined to be greater than 2000 mg/kg of body weight for both males and females.
This study received a Klimisch score of 1 and is classified as reliable without restrictions because it was conducted according to or similar to guideline study OECD TG 402.
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