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EC number: 201-173-7 | CAS number: 79-06-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
- Principles of method if other than guideline:
- ASTM, 1984b. Proposed New Standard Practice for conducting Life-cycle Toxici ty Tests with Saltwater Mysids. Subcommittee E.47.01, May, 1984.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Acrylamide
- EC Number:
- 201-173-7
- EC Name:
- Acrylamide
- Cas Number:
- 79-06-1
- Molecular formula:
- C3H5NO
- IUPAC Name:
- prop-2-enamide
- Details on test material:
- - Name of test material (as cited in study report): acrylamide monomer (2-propenamide; CAS No. 79-06-1)
- Physical state: whi te crystalline solid
- Analytical purity: Ultra Pure Electrophoresis Grade 99.9%
- Lot/batch No.: Lot 44569
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- SOLVENT /CARRIER: Deionized water
Test organisms
- Test organisms (species):
- Americamysis bahia (previous name: Mysidopsis bahia)
- Details on test organisms:
- TEST ORGANISM
- Common name: Mysid shrimp
- Strain/clone: Mysidopsis bahia
- Source: Bionomics Culture,
- Age of parental stock : ≤ 26 hours old
The test organisms were held in natural seawater at 30 parts per thousand (0/00) salinity and 25°C prior to distribution into the test aquaria.
Study design
- Test type:
- flow-through
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- Not measured
- Test temperature:
- 22.9-26.9 °C (mean 24.8±0.6 °C)
- pH:
- 8.0-8.1
- Dissolved oxygen:
- 5.3-7.2 mg/L ( >80% of saturation)
- Salinity:
- mean 30± 1 0/00 (28-31 0/00)
- Nominal and measured concentrations:
- Targeted nominal test concentrations: 0.09, 0.19, 0.38, 0.78, 1.50, 3.0 and 6.0 mg/L
Mean measured test concentrations: 0.06, 0.12, 0.24, 0.45, 1.02, 2.04 and 4.40 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material: aquarium with fill volume of 9 L . The retention chambers were glass Petri dishes to which a 15-centimeter high nylon screen collar was attached with silicone sealant.
- Aeration: no
- Type of flow-through: proportional diluter
- Renewal rate of test solution (frequency/flow rate): Each diluter was constructed to deliver 0.5 L/cycle/test aquarium at a dilution rate of 50. The average number of diluter cycles was approximately 6.1 or 6.7 per hour, providing approximately 8-9 volume replacements per aquarium every 24 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding during test: live brine shrimp (Artemia salina) nauplii daily
TEST MEDIUM / WATER PARAMETERS
- Source: Seawater (natural, filtered from Big Lagoon)
- Intervals of water quality measurement: The results of routine water quality analyses for metals, nutrients, pesticides, PCB's and petroleum hydrocarbons. Test dilution water quality was also biologically monitored in the laboratory through the maintenance of continuous cultures of mysids. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.04 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality
- Remarks:
- parental F0
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.04 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality
- Remarks:
- Offspring F1
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: from 8 to 22% in test concentrations >2.04 mg/L. Mortalities at these concentrations were not significantly different from the control mortality of 22%. At test termination, mortality in the highest concentration tested, 4.40 mg/L, reached 45% which was statistically greater than the control.
- No. of offspring produced per day per female: The mean number of offspring produced per female reproductive day ranged from 0.13 to 0.50 with no apparent correlation to acrylamide concentration; mean number of offspring in the control per female reproductive day was 0.33.
- Body length and weight of parent animals: Average dry weights of male mysids ranged from 0.42 mg at 4.40 mg/L acrylamíde to 0.55 mg in the control seawater. Male mysids exposed to ≥0.12 mg/L acrylamide had dry weights which were significantly less when statistically compared to the dry weights of control male mysids. Average dry weights of female mysids ranged from 0.47 mg at 4.40 mg/L acrylamide to 0.62 mg in the control and 0.24 mg/L test concentration. Female mysids exposed to ≥1.02 mg/L acrylamide had dry weights which were significantly less when statistically compared to the dry weights of control female mysids. Dry weight data of females probably do not accurately reflect growth, because of the possible presence of Artemia nauplii in their brood pouches. As Artemia were not removed prior to weight measurements, use of these data for setting criteria standards is not recommended by this laboratory.
- Number of males and females (parental): 40 per concentration
- Time to first brood release or time to hatch: There was no significant delay in time to release of the first brood at any test concentration. Time to first brood release in all replicate control and test aquaria ranged from 15 to 24 days with 84% of all first brood releases occurring between 15 and 20 days of exposure. Mean times to first brood release were not apparently concentration-related and ranged from 16 days in 0.24 mg/L to 21 days in 1.02 mg/L acrylamide
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Mysid reproduction was not adversely effected at any tested concentration. The NOEC was determined to be 2.04 mg/L for both parental (F0) and offspring (F1) survival.
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