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EC number: 206-058-5 | CAS number: 298-12-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.11 (Mutagenicity - In Vivo Mammalian Bone-Marrow Chromosome Aberration Test)
- Version / remarks:
- rules of GLP practice (Fed. Register, 22.12.78 and 11.04.80)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Glyoxylic acid
- EC Number:
- 206-058-5
- EC Name:
- Glyoxylic acid
- Cas Number:
- 298-12-4
- Molecular formula:
- C2H2O3
- IUPAC Name:
- 2-oxoacetic acid
- Details on test material:
- - Name of test material (as cited in study report): HF 0021 , Glyoxylic Acid 50%
- Physical state: clear, yellowish liquid
- Composition of test material, percentage of components: 50 % aqueous solution
- Lot/batch No.: Bac 203 A (5.10.82)
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River France breeding center
- Age at study initiation: 11-12 weeks
- Housing: animalös were kept 2 and/or 4 to a sterilisable polycarbonate cage
- Diet: pellet feed ad libitum
- Water: sterilised by filtration (millipore) ad libitum
- Acclimation period: 11 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 2 °C
- Humidity (%): 50 ± 20 %
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - clear, sterile pyogen-free water
- Details on exposure:
- Administraton:
Oral route (negative control and HF0021):
- orally by intubation in the volume of 25 ml/kg bw using a stainless steel, curved oesophagial cannula 16/10 mm in diameter and
40 mm long mounted on aq syringe
Intraperitoneal route (positive controls):
- intraperitoneally using a disposable 25 G needle 0.5 mm in diameter and 1.6 mm long - Duration of treatment / exposure:
- 48 hours
- Frequency of treatment:
- - 2 doses were administererd at an intervall of 24 hours, in the morning between 8.30 and 9.30 a.m.
- Post exposure period:
- 24 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 183, 366 or 914 mg/kg bw
Basis:
nominal conc.
gavage
- No. of animals per sex per dose:
- 4
- Control animals:
- yes
- yes, concurrent vehicle
- Positive control(s):
- - benzo(a)pyrene : 500 mg/kg bw
- cyclophosphamide: 100 mg/kg bw
- Route of administration: intraperitoneally
Examinations
- Tissues and cell types examined:
- 1) Hematological investigations
- a blood sample was collected by puncture of the retro-orbital sinus before sacrifice
performed: red blood count, haematokrit, haemoglobin, mean corpuscular volume, white blood count
2) Counting the micronuclei:
- femurs were removed after killing and bone marrow smears were prepared on a slide and stained
- The counts are based on examination of 1000 polychromatic erythrocytes - Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
The doses were set at 1/2, 1/5 and 1/10 of the LD50 of HF 0021 administered by oral route to the mouse
- Statistics:
- The values obtained for treated animals were compared with those obtained for control animals by means of a Student' t test.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Remarks:
- (but highest dose level corresponded to half ot the mouse LD50)
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- The substance HF 0021 (Glyoxylic Acid 50 %) was tested for mutagenicity in the mouse using the micronucleus test.
The results of the haematological investigations showed that the red cell parameters were normal for the animals treated with HF 0021.
The number of micronuclei found in the polychromatophilic erythrocytes of animals treated with the test substance was no greater than the number found in the negative control group.
The mutagenicity of benzo(a)pyrene and of cyclophophamide was confirmed by this test.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the experimental conditions, the substance HF 0021 (Glyoxylic Acid 50%) was found to have no mutagenic properties. - Executive summary:
In a Crl:Cobs CD-1 (1 CR) mouse bone marrow micronucleus assay, 4 animals/sex/dose were treated by gavage with Glyoxylic Acid 50% at doses of 0, 183, 366, or 914 mg/kg bw. Bone marrow cells were harvested 24 hours post-treatment. The vehicle was water. The highest dose corresponded to 1/2 of the mouse LD50 and thus represents the maximum tolerable dose.
There were no signs of toxicity during the study. The positive controls (Benzo(a)pyrene and Cyclophosphamide) induced the appropriate response. There was not a significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow up to the dose of 914 mg/kg bw after treatment time.
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