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EC number: 700-422-1 | CAS number: 61320-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial forward mutation assay
Test material
- Reference substance name:
- methyl 5-amino-4-cyano-3-methylthiophene-2-carboxylate
- EC Number:
- 700-422-1
- Cas Number:
- 61320-65-8
- Molecular formula:
- C8 H8 N2 O2 S
- IUPAC Name:
- methyl 5-amino-4-cyano-3-methylthiophene-2-carboxylate
- Details on test material:
- content: 99.1 %
batch no. BOS 2589
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix of livers from adult male Sprague-Dawley rats induced with Aroclor 1254a
- Test concentrations with justification for top dose:
- plate incorporation methodology: 0 (solvent control), 5000, 1600, 500, 160 or 50 µg/plate
due to precipitation at 5000 µg/plate:
preincubation methodology: 0 (solvent control), 2000, 1000, 500, 250. 125 µg/plate - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: Sodium azide, Nitrofurantoin, 4-Nitro-1,2-phenylene diamine, Mitomycin C, Cumene hydroperoxid, 2-Aminoanthracene
- Details on test system and experimental conditions:
- as described in the respective OECD Guideline:
plate incorporation methodology
preincubation methodology - Evaluation criteria:
- A reproducibel and dose related increase in mutant counts of at least one strain is considered to be positive result. For TA1535, TA100, TA1537 and TA98 thid indrease should be about twice tht of the negative controlls. For TA102 an increase of about 100 mutants should be reached. Otherwise, the result is evaluated as negative.
However, these criteria may be overruled by good scientific judgement.
In case of questionable results, investigations should should continue, possibly with modifications until a final evaluation is possible. - Statistics:
- no
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Remarks:
- 5000 µg/plate (plate incorporation assay), 2000 µg/plate (preincubation assay)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
Diazokomponente Rot B was investigated using the Salmonella/microsome test according to OECD TG 471 using TA1535, TA100, TA1537, TA98 and TA 102 in the presence and in the absence of a metabolic activation system. Initially the plate incorporation methodology was used and concentrations up to and including 5000 µg/plate. Due to precipitation at 5000 µg/plate the following preincubation methodoloy was done with concentrations up to and including 2000 µg/tube showing predipitation from 1000 µg/tube onwareds. Evidence of mutagenic activity of Diazokomponente Rot B was not seen. No biologically relevant increase in the mutant count in comparison th the negative controls was observed in any of the strains tested with and without S9 -mix in the plate incorporation as well as in the preincubation modification under the experimental conditions applied. The positive controls were functional.
Therefore, Diazokomponente Rot B was considered to be non-mutagenic without and with S9-mix in the plate incorporation as well as in the preincubation modification of the Salmonella / microsme test (Bayer 2011).
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