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EC number: 200-860-9 | CAS number: 75-31-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Only 30 min exposure time; only 2 concentrations tested; however, supported by tests on ready and inherent biodegradability (no microorganism toxicity observed.
- Justification for type of information:
- Exposure time in this test was limited to 30 minutes. However, available biodegradation screening tests (see cross-references) support this study with regard to long-term exposure of microorganisms: neither at 100 mg/L nor at 656 mg/L initial test item concentration was any relevant microorganism toxicity observed (fast and thorough mineralisation).
- Reason / purpose for cross-reference:
- reference to other study
- Remarks:
- Ready biodegradability
- Reason / purpose for cross-reference:
- reference to other study
- Remarks:
- Inherent biodegradability (oxygen consumption)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted in April 1984
- Deviations:
- yes
- Remarks:
- only 2 concentrations tested
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Based on a relative density of 0.69, 181 µL and 362 µl were directly added to the test medium to arrive at test item concentrations of 500 and 1000 mg/L (250 mL total volume).
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge from a laboratory wastewater plant treating municipal and artificial sewage. Concentration of dry substance 1 g/L .
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Hardness:
- no data
- Test temperature:
- 20 ± 2 °C
- pH:
- According to the report, pH was adapted to pH 7.0 to 8.0 before inoculum was added.
However, in the results table, pH values of 10.9 (500 mg/L) and 11.2 (1000 mg/L) are given (see attached illustration). This is contradictory and it is not clear what actual pH prevailed during the study. - Dissolved oxygen:
- no data
- Salinity:
- fresh water
- Nominal and measured concentrations:
- 500 and 1000 mg/L (nominal)
- Details on test conditions:
- Incubation temperature: 20 +/-2 °C;
Synthetic medium: 100x concentrated OECD medium, 8 mL per sample (final volume 250 mL); - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 600 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC20 approx. 1.5 mg/L, EC50 approx. 10 mg/L, EC80 approx. 80 mg/L - Validity criteria fulfilled:
- yes
- Remarks:
- according to OECD 209 (1984)
- Conclusions:
- In a valid (RL 2) activated sludge respiration inhibition test (OECD 209, 1984) performed compliant to GLP the following results were obtained:
EC20 (30 min) ca. 600 mg/L;
EC50 (30 min) > 1000 mg/L (27 % inhibition observed relative to control).
The study is supported regarding long-term microorganism toxicity by available biodegradation screening tests, where no microorganism toxicity was observed up to 656 mg/L test item (fast and thorough mineralisation). - Executive summary:
In a valid (RL 2) activated sludge respiration inhibition test (OECD 209, 1984) performed compliant to GLP the following results were obtained:
EC20 (30 min) ca. 600 mg/L;
EC50 (30 min) > 1000 mg/L (27 % inhibition observed relative to control).
Contradictory information is given in the report with regard to the pH prevailing during the test. While the text section states that neutralization were performed before addition of inoculum, the results table gives pH values of 10.9 (500 mg/L) and 11.2 (1000 mg/L) which would make pH induced toxicity probable.
While the exposure time was limited to 30 minutes instead of 3 hours demanded today as the minimum exposure time, the study is supported regarding long-term microorganism toxicity by available biodegradation screening tests, where no microorganism toxicity was observed up to 656 mg/L test item (fast and thorough mineralisation).
Altogether, the data allow to reliably conclude on a rather low STP microorganism toxicity, which may have been induced solely due to the alkaline pH produced by isopropylamine (pKa 10.8 for alkaline functionality). Accordingly, an approximate EC10 of 300 mg/L is derived from the data and is taken as the relevant key value for chemical safety assessment.
Reference
Description of key information
In a valid (RL 2) activated sludge respiration inhibition test (OECD 209, 1984) performed compliant to GLP the following results were obtained:
EC20 (30 min) ca. 600 mg/L;
EC50 (30 min) > 1000 mg/L (27 % inhibition observed relative to control).
The study is supported regarding long-term microorganism toxicity by available biodegradation screening tests, where no microorganism toxicity was observed up to 656 mg/L test item (fast
and thorough mineralisation).
Accordingly, an approximate EC10 of 300 mg/L is derived from the data on respiration inhibition and is taken as the relevant key value for chemical safety assessment.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 300 mg/L
Additional information
In a valid (RL 2) activated sludge respiration inhibition test (OECD 209, 1984) performed compliant to GLP the following results were obtained:
EC20 (30 min) ca. 600 mg/L;
EC50 (30 min) > 1000 mg/L (27 % inhibition observed relative to control).
Contradictory information is given in the report with regard to the pH prevailing during the test. While the text section states that neutralization were performed before addition of inoculum, the results table gives pH values of 10.9 (500 mg/L) and 11.2 (1000 mg/L) which would make pH induced toxicity probable.
While the exposure time was limited to 30 minutes instead of 3 hours demanded today as the minimum exposure time, the study is supported regarding long-term microorganism toxicity by available biodegradation screening tests, where no microorganism toxicity was observed up to 656 mg/L test item (fast and thorough mineralisation).
Altogether, the data allow to reliably conclude on a rather low STP microorganism toxicity, which may have been induced solely due to the alkaline pH produced by isopropylamine (pKa 10.8 for alkaline functionality). Accordingly, an approximate EC10 of 300 mg/L is derived from the data on respiration inhibition and is taken as the relevant key value for chemical safety assessment.
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