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Diss Factsheets
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EC number: 939-382-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
Short-term toxicity in freshwater from each of the three trophic levels (on fish, aquatic invertebrate and aquatic plants) was evaluated considering data on Direct Black 22 (DBk22) and data on structural analogues.
No adverse effects were recorded at the limit concentrations tested in studies on short-term to fish and aquatic invertebrates, as well as in tests conducted in order to investigate the toxicity to microorganisms.
The IC50 recorded at 72 hours in the test on algae was 38.5 mg/L of active material (Riva, 2000), similar to results obtained in the study on the analogous Similar Substance 01 (Dolezavola, 1994). Nevertheless coloured substances are often assumed to behave differently in the algal growth inhibition test due to shading of the algae.
In order to clarify the (eco)toxicological properties related to toxicity for aquatic plants, it was considered a read across test with the analogue Similar Substance 01 using Lemna gibba as a test organism (Caduff, 2012), as the test substance is a dyestuff which could influence the photosynthesis capacity of algae cells in the test solution. The results reported on the Similar Substance 01 (Caduff, 2012) confirm the non-toxicity of the dye that is a structural analogue of DBk22.
The differences in outcomes of the two studies are most likely due to the shading effects of coloured water in the algae test.
From this point of view the results recorded in Lemna study are more reliable and, considering the structural analogy of the two dyes, DBK22 is expected to be non-toxic to aquatic plants.
.Long-term toxicity was not tested because direct and indirect exposure is unlikely due to the risk management measures that will be applied at industrial level. The risk management measures to avoid any release of the substance directly in the environment are discussed in detail within the exposure scenario; furthermore DBk22 has a low potential for bioaccumulation and aquatic toxicity is unlikely to occur as indicated by the short-term aquatic testing results. Therefore, long-term toxicity testing is not necessary, because the chemical safety assessment according to Annex I does not indicate the need to investigate further the effects on aquatic organisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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