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EC number: 407-550-4 | CAS number: 69184-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Mar- 16 Oct 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Adopted in 1981, updated in 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- EEC Directive 67/548 Annex V C.1 (84/449/EEC). Adopted in 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- EC Number:
- 407-550-4
- EC Name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- Cas Number:
- 69184-17-4
- Molecular formula:
- Hill formula: C8 H7 Cl2 F N2 O3 CAS formula: C8 H7 Cl2 F N2 O3
- IUPAC Name:
- methyl 2-[(4-amino-3,5-dichloro-6-fluoropyridin-2-yl)oxy]acetate
- Details on test material:
- - Name of test material (as cited in study report): Fluroxypyr methyl ester
- Physical state: solid, grey powder
- Analytical purity: 90.8%
- Lot/batch No.: C-3B
- Stability under test conditions: ensured by daily renewal of test media and verified by chemical analysis
- Storage condition of test material: in darkness at 4°C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Duplicate samples (each comprising 100.0 mL test medla plus 1.0 ml 50% v/v orthophosphoric acid (aqueous) in 100 ml glass bottles) were
received from the Unit of Fish Toxicology between 22 and 26 March 1991. One of each sample pair was stored at -20 °C. The retained sample was shaken and a sub-sample transferred by bulb pipette (5.0 mL) to a volumetric flask (10.0 mL). The sample was made to volume with acetonitrile and shaken. An aliquot was passed through a 0.45 micron membrane filter and retained for analysis.
- Samples were collected from control and test concentrations at 0 and 72 hours (fresh media) and at 24 and 96 hours (expired media)
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The preliminary solution was created by adding the test substance and 1% Tween 80-acetone
- Controls: blank and vehicle control
- Chemical name of vehicle: acetone
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Swaffham, United Kingdom
- Length at study initiation (mean, SD): 3.8 cm, 0.2 cm
- Weight at study initiation (mean, SD): 0.67 g, 0.14 g
- Feeding during test: no
ACCLIMATION
- Acclimation period: the fish stock was held since 26 February 1991 and acclimatised to test conditions since 4-22 March 1991
- Acclimation conditions (same as test or not): yes
- Type of food: commercial trout pellets
- Feeding frequency: daily, discontinued 24 h prior to test
- Health during acclimation (any mortality observed): no mortalities were observed during the acclimation period
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- ~ 350 mg/L as CaCO3
- Test temperature:
- 14 ± 1 °C
- pH:
- 7.9-8.0
- Dissolved oxygen:
- ≥ 10.2 mgO2/L
- Nominal and measured concentrations:
- Nominal: control, 1.0, 1.8, 3.2, 5.6 and 10 mg/L
Measured: control, 0.81, 1.5, 2.5, 4.6 and 7.8 mg/L
The test substance concentration ranged from 87-96% of nominal concentrations for fresh media and 67-87% for expired media - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria containing 20 L of test medium
- Aeration: yes, via narrow bore glass tubes
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.34 g bw/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water, dechlorinated by the addition of sodium thiosulphate
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality and behaviour at 3, 6, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 2.5-4.6 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 2.6-3.8 mg/L
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 1.5-2.5 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 1.4-1.8 mg/L
- Details on results:
- - Behavioural abnormalities: Loss of equilibrium, increased pigmentation and lethargic state were observed at concentrations from 1.8 mg/L and above
- Mortality of control: no mortalities were reported in either control or solvent control
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1. Cumulative mortality data for rainbow trout exposed for 96 hours to fluroxypyr methyl ester
Test concentration (mg/L)
Cumulative mortality
(initial population: 10)
3 h
6 h
24 h
48 h
72 h
96 h
Control
0
0
0
0
0
0
Solvent control
0
0
0
0
0
0
1.0
0
0
0
0
0
0
1.8
0
0
0
0
0
2
3.2
0
0
0
3
10
10
5.6
0
0
10
10
10
10
10.0
0
0
10
10
10
10
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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