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EC number: 219-147-9 | CAS number: 2373-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Guideline and GLP compliant study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 100 mg test item /L
- Sampling method: For the determination of the actual test item concentrations, duplicate samples were taken from each treatment at the start and at the end of the test (after 48 hours). For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium was prepared by dissolving 50.1 mg of test item completely in 500 mL of test water using ultrasonic treatment for 15 minutes and intense stirring for 15 minutes at room temperature. The test medium was prepared just before introduction of the daphnids (i.e. start of the test).
- Eluate: no
- Differential loading: no
- Controls: dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Concentration of vehicle in test medium (stock solution and final test solution): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no, recoveries of freshly prepared and aged test solutions were 88% at both sampling times, indicating that no significant precipitation could have been occured. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Strain: clone 5
- Source: A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield / UK in 1992. Since that time, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the test.
- Age at study initiation (mean and range, SD): At the start of the test, the organisms used in the test were 6 24 hours old and were not first brood progeny.
- Method of breeding: During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at Harlan Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle / Germany).
- Feeding during test: no
- Food type: NA
- Amount: NA
- Frequency: NA
ACCLIMATION
- Acclimation period: The test was conducted in AAP Medium. The parental daphnids are cultivated in water of the same quality (in respect to pH, main ions, and total hardness). Reconstituted test water (AAP medium) was prepared according to OECD Test Guideline No 201
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at Harlan Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle / Germany).
- Feeding frequency: three times a week
- Health during acclimation (any mortality observed): no health problems reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 15 mg/L as CaCO3
- Test temperature:
- The water temperature during the test was between 20 - 21 °C.
- pH:
- The pH values of the test medium and control were 7.0
- Dissolved oxygen:
- At the beginning and end of the test period, the dissolved oxygen concentration in the test medium and control was at least 7.4 mg/L.
- Nominal and measured concentrations:
- nominal concentrations: 0 (control), 100 mg test item/L
measured concentrations Control < LOQ, 100 mg/L: 88 mg/L in the freshly prepared and aged test solutions - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100-mL glass beakers filled with 50 mL of test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 daphnia/10 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test was conducted in AAP Medium. The parental daphnids are cultivated in water of the same quality (in respect to pH, main ions, and total hardness). Reconstituted test water (AAP medium) was prepared according to OECD Test Guideline No 201.
- Culture medium different from test medium: No
- Intervals of water quality measurement: start and termination of the exposure.
OTHER TEST CONDITIONS
- Photoperiod: A 16-hour light to 8-hour dark cycle with a 30-minute transition period was used. Light intensity during the light period was approximately between 390 and 560 Lux.
- Light intensity: Light intensity during the light period was approximately between 390 and 560 Lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : NOEC, EC10, EC50 at 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: NA since limit test
- Range finding study: performed but results were not reported. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilization
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilization
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilization
- Details on results:
- - Behavioural abnormalities: none
- Other biological observations: not reported
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in September 2012 (48-hour EC50: 0.94 mg/L, Harlan Laboratories Study D64333) showed that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from 2000 to 2012: 0.43 1.1 mg/L).
- Reported statistics and error estimates:
- Since no immobilization was observed, the NOEC, EC0, EC50 and EC100 were determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48-hour-EC50 to daphnids: >100 mg/L.
- Executive summary:
In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Sodium di(1.3-dimethylbutyl) sulfosuccinate to Daphnia magna was determined in a static limit test according to EEC C.2 and OECD 202. The test was started with a control and a nominal concentrations of 100 mg test item. The concentrations were analyzed by HPLC analyses. The mean measured concentrations were 0 (control) and 88 mg/L for the nominal concentration of 100 mg/L at the start and end of the exposure. Since all values were > 80 % the results were based on nominal concentrations. For each test concentration and the control, 4 replicates with 5 daphinds each were set up in 100 mL vessels filled with 50 mL test solution. After 48 hours of exposure no immobilization was observed in the control and the 100 mg/L test solution. The corresponding 48 h EC10 and EC50 value are > 100 mg test item/L.
This study is considered to be acceptable for the risk assessment.
Reference
Table 1 Effect of Sodium di(1.3-dimethylbutyl) sulfosuccinate on the Mobility of Daphnia magna
Nominal |
No. of |
Immobilized |
Immobilized |
||
[mg/L] |
|
No. |
[%] |
No. |
[%] |
Control |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0 |
0 |
0 |
0 |
Description of key information
Daphnia 48-h: EC50 > 100 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 100 mg/L
Additional information
In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Sodium di(1.3-dimethylbutyl) sulfosuccinate to Daphnia magna was determined in a static limit test according to EEC C.2 and OECD 202. The test was started with a control and a nominal concentrations of 100 mg test item. The concentrations were analyzed by HPLC analyses. The mean measured concentrations were 0 (control) and 88 mg/L for the nominal concentration of 100 mg/L at the start and end of the exposure. Since all values were > 80 % the results were based on nominal concentrations. For each test concentration and the control, 4 replicates with 5 daphinds each were set up in 100 mL vessels filled with 50 mL test solution. After 48 hours of exposure no immobilization was observed in the control and the 100 mg/L test solution. The corresponding 48 h EC10 and EC50 value are > 100 mg test item/L.
This study is considered to be acceptable for the risk assessment.
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