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EC number: 300-339-7 | CAS number: 93925-37-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7 July 2009 - 20 August 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, without deviations from standard test guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
- EC Number:
- 300-339-7
- EC Name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
- Cas Number:
- 93925-37-2
- Molecular formula:
- C18H15O3P.C7H12O2.C4H6.S as an example
- IUPAC Name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l
- Sampling method: Water samples were taken from the control (replicates R1 – R2 pooled) and each loading rate WAF test group (replicates R1 – R2 pooled) at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours for quantitative analysis.
- Sample storage conditions before analysis: Samples were used immediately. Duplicate samples were taken and stored at -20 C for further analysis if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) solution preparation. The aqueous phase or WAF was removed by mid-depth siphoning method.
- Eluate: Not applicable.
- Differential loading: Water Accommodated Fractions (WAFs) loading rate.
- Controls: Untreated negative control and positive control which conducted every 6 months.
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test material to be present.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water Flea
- Strain: Daphnia magna
- Source: In house laboratory cultures
- Age at study initiation (mean and range, SD): 1st instar, less than 24 h old
- Weight at study initiation (mean and range, SD): no data available.
- Length at study initiation (length definition, mean, range and SD): no data available.
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data available.
- Peripheral shell growth removed prior to test initiation: no data available.
- Method of breeding: parthenogenises
- Feeding during test : The daphnids received no food during exposure
- Food type: Not applicable.
- Amount: Not applicable.
- Frequency: Not applicable.
ACCLIMATION
- Acclimation period: Not applicable.
- Acclimation conditions (same as test or not): Not applicable.
- Type and amount of food: Each culture was fed daily with a suspension of algae (Chlorella sp.).
- Feeding frequency: daily
- Health during acclimation (any mortality observed): Not applicable.
QUARANTINE (wild caught)
- Duration: Not applicable.
- Health/mortality: Not applicable.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
Test conditions
- Hardness:
- The reconstituted water had an approximate theoretical total hardness of 250 mg/l as
CaCO3. - Test temperature:
- 21ºC to 22ºC
- pH:
- There were no treatment related differences in pH (pH = 8.0).
- Dissolved oxygen:
- There were no treatment related differences in oxygen concentration (%ASV = 98~102%).
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Nominal loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml glass jars
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml glass jars containing approximately 250 ml of test preparation were used.
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable.
- Renewal rate of test solution (frequency/flow rate): the test media renewal at 24 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Duplicate test vessels were used for each test and control group
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): Not applicable.
- Biomass loading rate: No data available.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Total organic carbon: NDA
- Particulate matter: NDA
- Metals: NDA
- Pesticides: NDA
- Chlorine: NDA
- Alkalinity: NDA
- Ca/mg ratio: NDA
- Conductivity: NDA
- Culture medium different from test medium: No difference
- Intervals of water quality measurement: N/A
OTHER TEST CONDITIONS
- Adjustment of pH: Using NaOH or HCl were necessary
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods
- Light intensity: NDA
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The criterion of effect used was that Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8x
- Justification for using less concentrations than requested by guideline: NA
- RANGE FINDING STUDY
- Test concentrations: nominal loading rates of 1.0, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: No immobilisation was observed at the 1.0 mg/l loading rate WAF. However, immobilisation was observed at the 10 and 100 mg/l loading rate WAFs.
Based on this information loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l, using a stirring period of 23 hours followed by a 1-Hour standing period, were selected for the definitive test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (non-concurrent)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 8.4 - 13 mg/L WAF
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: NDA
- Observations on body length and weight: NDA
- Other biological observations: NDA
- Mortality of control: 0
- Other adverse effects control: None noted
- Abnormal responses: NDA
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None noted
- Effect concentrations exceeding solubility of substance in test medium: N/A - Results with reference substance (positive control):
- - Results with reference substance valid? Yes.
- Mortality: mortality was observed at concentration ≥ 0.56 mg/L
- EC50/LC50:
Time (h) 3
EC50 (mg/l) > 3.2
95% Confidence limits (mg/l) - no data
Time (h) 24
EC50 (mg/l) = 1.0
95% Confidence limits (mg/l) = 0.90 – 1.2
Time (h) 48
EC50 (mg/l) = 0.78
95% Confidence limits (mg/l) = 0.68 - 0.88 - Reported statistics and error estimates:
- No data available
Any other information on results incl. tables
Cumulative Immobilisation data in the Range Finding Test
Nominal Loading Rate (mg/l) |
Cumulative ImmobilisedDaphnia(Initial Population: 10 Per Replicate) |
|
24 Hours |
48 Hours |
|
Control |
0 |
0 |
1.0 |
0 |
0 |
10 |
0 |
2 |
100 |
0 |
4 |
Cumulative Immobilisation Data in the Definitive Test
Nominal Loading Rate (mg/l) |
Cumulative Immobilised Daphnia (Initial Population: 10 Per Replicate) |
|||||||
24 Hours |
48 Hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
10 |
5.6 |
0 |
0 |
0 |
0 |
3 |
3 |
6 |
30 |
10 |
0 |
0 |
0 |
0 |
5 |
4 |
9 |
45 |
18 |
0 |
0 |
0 |
0 |
6 |
7 |
13 |
65 |
32 |
0 |
0 |
0 |
0 |
9 |
9 |
18 |
90 |
56 |
0 |
0 |
0 |
0 |
10 |
10 |
20 |
100 |
100 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
Chemical analysis of test loading rates
Chemical analysis of the test preparations (see Appendix 3) at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period.
The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l. Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous.
The results of the chemical analysis showed measured concentrations in the definitive test that were lower than the measured Total Organic Carbon (TOC) levels observed in the pre-study validation of mixing period work. This was considered to be due to the analytical method employed only detecting the 399 ion whereas TOC analysis detected the total dissolved test material present in the WAF.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL*50 value of 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate at 48 hours was 1.8 mg/l loading rate WAF.
- Executive summary:
Introduction.A study was performed to assess the acute toxicity of the test material to daphnia magna followed that described in the OECD 202.
Methods.Following a preliminary range-finding test, twenty daphnids (2 replicates of
10 animals) were exposed to Water Accommodated Fractions (WAFs) of the test material over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l for 48 hours at a temperature of 21ºC to 22ºC under semi-static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.
Results.The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading rates was 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/l loading rate WAF.
Chemical analysis of the test preparations at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period.
The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l.
Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous.
The dissolved test material may have been one or several components of the test material. Given that toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50 for the reference material to Daphnia magna based on nominal concentrations was 0.78 mg/l with 95% confidence limits of 0.68 – 0.88 mg/l. The No Observed Effect Concentration was 0.32 mg/l.
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