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Diss Factsheets
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EC number: 913-635-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Taken from OECD SIDS (OECD Guideline study)
- Justification for type of information:
- See attached file
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Supplier/Source (Strain Number): ATCC 22662
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 900 mg/L
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 1 000 mg/L
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4 000 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 2 700 mg/L
- Basis for effect:
- growth rate
- Conclusions:
- The EC50 (based on growth rate) to Pseudokirchneriella subcapitata was determined to be > 4000 mg/L.
- Executive summary:
The EC50 (based on growth rate) to Pseudokirchneriella subcapitata was determined to be > 4000 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline study without detailed description..
- Justification for type of information:
- See attached file
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
n.a. - Analytical monitoring:
- no
- Details on sampling:
- n.a.
- Vehicle:
- no
- Test organisms (species):
- Nitzschia sp.
- Details on test organisms:
- Described as Nitschia linearis
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 120 h
- Post exposure observation period:
- n.a.
- Hardness:
- 100 mg/L as CaCO3
- Test temperature:
- not specified
- pH:
- not specified
- Dissolved oxygen:
- not specified
- Salinity:
- not specified
- Nominal and measured concentrations:
- not specified
- Details on test conditions:
- not specified
- Reference substance (positive control):
- no
- Duration:
- 120 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 430 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Results with reference substance (positive control):
- n.a.
- Reported statistics and error estimates:
- No statistic described, no error estimates provided.
- Conclusions:
- EC50 (120 hr, Nitzschia linearis, cell counts) = 2430 mg/L
- Executive summary:
The study tested EC50 /LC50 for fish (Lepomis macrochirus), snail (Physa heterostropha), and algae (Nitzschia linearis, diatom) to conclude on relative sensitivity. Sodium chloride was tested for fish and algae toxicity only. The 120 hr EC50 with NItzschia linearis based on cell count was determined as 2430 mg/L. Bluegill was less sensitive with 96hr-LC50=12946 mg/L (not summarised in this dataset).
Referenceopen allclose all
Table: Measured algal cell densities
--------------------------------------------------
Nominal test Cell density x 10^4 (cells/ml)
concentration
--------------------------------------------------
(mg/l) Day 1 Day 2 Day 3
--------------------------------------------------
0 2.494 15.24 77.62
0 2.324 13.05 61.49
0 2.282 14.10 68.88
0 2.409 15.44 74.73
0 2.536 16.15 81.91
0 2.367 13.62 63.51
1000 1.859 9.106 39.74
1000 2.240 13.43 70.85
1000 2.071 12.02 60.09
1400 1.902 10.99 48.54
1400 1.859 10.19 46.84
1400 1.817 10.86 48.29
2000 1.859 17.49 33.98
2000 2.113 15.82 43.79
2000 1.902 12.52 32.03
2800 1.986 12.52 44.04
2800 2.155 8.389 30.73
2800 1.732 9.645 26.78
4000 1.775 8.299 19.88
4000 1.563 6.336 19.26
4000 1.732 11.93 18.35
----------------------------------------------------
Table: Percent biomass inhibition per concentration
----------------------------------------------------
Nominal test Percent growth inhibition
concentration
----------------------------------------------------
(mg/l) Area under the growth curve
----------------------------------------------------
0 0
1000 21.2
1400 33.6
2000 35.6
2800 47.1
4000 64.9
----------------------------------------------------
Table: Water parameters of test solutions
----------------------------------------------------
concentration Temperature pH
CaCl2 (mg/l) (°C)
----------------------------------------------------
0 22.2 8.5
1000 22.2 7.5
1400 22.4 7.8
2000 22.2 7.9
2800 22.3 7.9
4000 22.3 7.8
----------------------------------------------------
Table: EC50 and EC20
----------------------------------------------------
Hours of EC50 EC20
exposure (mg/l) (mg/l)
----------------------------------------------------
72 2900 1000
----------------------------------------------------
Table: Percent growth rate inhibition per concentration
-------------------------------------------------------
Nominal test Percent growth inhibition
concentration
(mg/l) Growth rate
-------------------------------------------------------
0 0
1000 4.9
1400 9.9
2000 15.5
2800 17.6
4000 31.0
-------------------------------------------------------
Table: EC50 and EC20
---------------------------------------
Hours of EC50 EC20
exposure (mg/l) (mg/l)
---------------------------------------
72 > 4000 2700
---------------------------------------
Description of key information
The reaction mass contains c.a. 68% of calcium chloride and 30% of sodium chloride. The EC50 of the reaction mass was defined based on a worst case (i.e. the lowest EC50 of the two constituents was selected to reflect the EC50 of the reaction mass). Accordingly, the EC50 for algae and cyanobacteria of the reaction mass of calcium chloride and sodium chloride is 2430 mg/L (based on the EC50 of sodium chloride).
As no NOEC for sodium chloride could be derived from the key study, the EC20 for calcium chloride was selected (i.e. 1000 mg/L).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2 430 mg/L
- EC10 or NOEC for freshwater algae:
- 1 000 mg/L
Additional information
No experimental data are available to assess the short-term toxicity of the reaction mass of calcium chloride and sodium chloride to algae and cyanobacteria. Nevertheless, reliable information is available for calcium chloride (CaCl2) and sodium chloride (NaCl). The acute toxicity towards algae and cyanobacteria of the constituents of the reaction mass (i.e. CaCl2 and NaCl) is known and the LD50 of the substance can thus be predicted. To this end, the key study assessing the acute toxicity of both compounds identified in the REACH registration dossier was selected.
Three studies are available from published literature on acute toxicity of sodium chloride to algae and cyanobacteria. The diatom Nitzschia linearis was found to be the most sensitive species with an EC50 (120 h, cell number) of 2430 mg/L. Unfortunately, the article does not provide sufficient information to conclude on a No Observed Effect Concentration for Nitzschia linearis. Only this key study was reported in the data of this dossier.
For calcium chloride, a single study with the freshwater algae, Pseudokirchneriella subcapitata (previously named as Selenastrum capricornutum) was conducted according to OECD guideline 201. The 72-hour EC50 and EC20 obtained on the basis of biomass from the study were 2900 and 1000 mg/L, respectively.
The reaction mass of calcium chloride and sodium chloride contains c.a. 68% of calcium chloride and 30% sodium chloride. The EC50 of the reaction mass was defined based on a worst case (i.e. the lowest EC50 of the two constituent was selected to reflect the EC50 of the reaction mass). Accordingly, the EC50 for algae and cynaobacteria of the reaction mass of calcium chloride and sodium chloride is 2430 mg/L. As no NOEC for sodium chloride could be derived from the key study, the EC20 for calcium chloride was selected (i.e. 1000 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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