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EC number: 254-463-0 | CAS number: 39455-80-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reasonably well descrtibed study. No GLP is reported.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of genotoxicity of oral exposure to tetravalent vanadium in vivo.
- Author:
- Villani, P.; et al.
- Year:
- 2 007
- Bibliographic source:
- Toxicology letters, 170, 11-18.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- not specified
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- vanadium oxide sulphate pentahydrate
- IUPAC Name:
- vanadium oxide sulphate pentahydrate
- Reference substance name:
- 123334-20-3
- Cas Number:
- 123334-20-3
- IUPAC Name:
- 123334-20-3
- Reference substance name:
- Vanadium oxide sulphate
- EC Number:
- 248-652-7
- EC Name:
- Vanadium oxide sulphate
- Cas Number:
- 27774-13-6
- IUPAC Name:
- oxovanadium(2+) sulfate
- Test material form:
- other: solid
- Details on test material:
- - Name of test material (as cited in study report): Vanadyl sulphate hydrate
- Physical state: solid
- Analytical purity: 99.99%
No further details are given.
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan s.r.l. (Italy)
- Assigned to test groups randomly: yes
- Diet: ad libitum; laboratory rodent diet
- Water: ad libitum
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 55 +/- 15
- Photoperiod: 12 hours dark/light cycle
No further details are given.
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- - Vehicle(s)/solvent(s) used: distilled water
No further details are reported. - Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: Tap water was used to dilute VOSO4 stock solutions and administered as such to control animals.
DIET PREPARATION
not applicable - Duration of treatment / exposure:
- 5 weeks
- Frequency of treatment:
- daily
- Post exposure period:
- no data
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
2 mg/L
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
10 mg/L
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
100 mg/L
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
500 mg/L
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
1000 mg/L
Basis:
nominal in water
- No. of animals per sex per dose:
- 8 to 10 male mice were randomly assigned to each treatment group.
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- methylmethanesulfonate
- Doses / concentrations: 80 mg/kg body weight
No further details are given.
Examinations
- Tissues and cell types examined:
- bone marrow
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: preliminary test results
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): no further details
DETAILS OF SLIDE PREPARATION: Bone marrow cells were obtained by flushing both femours with phosphate buffer saline (PBS). After centrifugation and resuspension in PBS, the cell suspension was used for micronucleus assay.
Some drops of bone marrow cell suspension were spread on slides. For each animal 4 slides were prepared. Air-dried smears were then fixed in absolute methanol at room temperature for 5 minutes and stained with 5% solution of Giemsa in 0.01M phosphate buffer at pH 6.8 for 20 minutes to differentiate bone marrow polychromatic (PCE) from normochromatic erythrocytes (NCE).
METHOD OF ANALYSIS: Slides were coded and blind scored using a brightfield microscope. In the first experiment the frequency of micronucleated PCEs was evaluated analysing 2000 cells/animal (1000 cels each of two scorers); 1000 cells/animal were analysed in the positive control group.
OTHER: To assess bone marrow toxicity, the percentage of PCEs was evaluated over 1000 total erythrocytes (PCEs + NCEs). - Evaluation criteria:
- No details are reported.
- Statistics:
- Means of different experimental groups were compared by two-tailed Student's t-test. The limit of statistical significance was set at p=0.05.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- No increase in the incidence of MnPCEs was observed in the mice treated with doses from 10 to 1000 mg/L.
- Toxicity:
- no effects
- Remarks:
- No treatment related changes in the PCE/NCE ratio.
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
No details are reported, but the top dose was selected as maximum tolerated dose on the basis of the results of a preliminary range-finding experiment and of literature data (ciranni et al. 1995).
RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): No increase in the incidence of micronucleated polychromatic erythrocyte (MnPCEs) was observed in bone marrow cells of mice treated with vanadyl sulphate in a range of doses from 10 to 1000 mg/L. The negative result obtained in the first experiment was confirmed in the second experiment.
- Ratio of PCE/NCE (for Micronucleus assay): The determination of the PCE/NCE ratio did not show dose-related deviations in this parameter indicating bone marrow toxicity in treated mice.
General toxicity: Daily water consumption was significantly reduced in the two high dose groups (1000 and 500 µg/L), possibly because of the effect of vanadyl sulphate on the palatability of drinking water. This led to a less than proportional increase in vanadium intake in the high dose groups. Consequently, in the second experiment an increased water consumption was observed in the low dose group (2 mg/L). No significant differences in food consumption were observed among experimental groups. Treatment did not affect body weight gain in the 5 weeks of exposure.
Vanadium distribution: In the first experiment a dose-related, linear increase of vanadium content was observed in bone tissues (R²=0.95 and R²=0.96 in femours and in tibias, respectively). Slightly higher vanadium concentrations were measured in bone in the second experiments at the dose of 10 mg/L: however, when data from the two experiments were compared by t-test, no statistical significant differences at 0.05 level were observed.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the given test conditions reported, vanadyl sulphate pentahydrate is not mutagenic in the bone marrow micronucleus assay. - Executive summary:
In this reference, the genotoxic effects induced in vivo by subacute oral exposure to vanadyl sulphate (VOSO4) were investigated. To this aim male CD1 mice were administered with VOSO4 in drinking water over the dose range 2 -1000 mg/L for 5 weeks. At the end of treatment, micronuclei in bone marrow polychromatic erythrocytes were determined. Tissue distribution of vandaium at sacrifice was determined by atomic absorption spectrometry.
The analysis of micronuclei did not reveal treatment related effects. Under the given test conditions reported, vanadyl sulphate pentahydrate is not mutagenic in the bone marrow micronucleus assay.
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