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EC number: 251-257-2 | CAS number: 32846-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Sodium 6-amino-5-[[2-[(cyclohexylmethylamino)sulphonyl]phenyl]azo]-4-hydroxynaphthalene-2-sulphonate
- EC Number:
- 251-257-2
- EC Name:
- Sodium 6-amino-5-[[2-[(cyclohexylmethylamino)sulphonyl]phenyl]azo]-4-hydroxynaphthalene-2-sulphonate
- Cas Number:
- 32846-21-2
- Molecular formula:
- C23H26N4O6S2.Na
- IUPAC Name:
- sodium 6-amino-5-({2-[cyclohexyl(methyl)sulfamoyl]phenyl}diazenyl)-4-hydroxynaphthalene-2-sulfonate
Constituent 1
- Specific details on test material used for the study:
- Sample Name : Tectilon Red 2B KWL
Product # : 01-135303-100-0
Batch # : E 525, DCT #80220
Material received : 9/25/78
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- New Zealand White rabbits approximately 8 to 11 weeks of age were, received in good health from our local supplier and remained in good health during the equilibration period in this laboratory. The rabbits were equilibrated for at least 7 days. The rabbits were individually housed in elevated wire mesh cages in temperature-controlled rooms reserved exclusively for rabbits on acute tests.
Each animal was identified by a uniquely numbered metal tag affixed to its ear. Purina Rabbit Chow and water from bottles were available ad libitum.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Remarks:
- Red Powder-used as 50 % weight/volume suspension in distilled water
- Details on dermal exposure:
- Twenty-four hours prior to dosing the backs of the rabbits were clipped free of fur with an Oster ANG-RA clipper head designed specifically for clipping rabbits. The rabbits were returned to their cages overnight. Just prior to dosing, the backs of even-numbered rabbits were abraded with a 21-gauge bent tip needle. The abrasions, made every 2 to 3 cm longitudinally, scratched the stratum corneum, but did not disturb the derma or produce bleeding.
The test material was applied to the backs of two male and two female rabbits at a dose of 2000 mg/kg. The test site was covered with gauze and the trunk was wrapped with impervious material for 24 hours. Following removal of the binder at 24 hours, the test site was washed with warm tap water. One hour after washing, the test sites were graded for skin irritation. Skin sites were read again at 7 and 14 days. - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 2 males and 2 females
- Control animals:
- no
- Details on study design:
- Body weights were recorded pretest and at 7 and 14 days. The rabbits were observed daily for 14 days for signs of toxicity and mortality. Necropsies were performed on all rabbits.
- Statistics:
- None
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One (animal #4) out of the 4 animals was found dead on day 8.
- Clinical signs:
- other: Yellow nasal discharge - Animal #2, days 4-6, 11-13 Mucus in stool - Animal #4, days 3-6 Diarrhea - Animal #4, days 3-7 Ptosis - Animal #4, days 6 & 7 Lethargy - Animal #4, day
- Gross pathology:
- Animal #1 and 3 - No gross pathological observations.
Animal #2 : yellow exudate, nose/mouth; dark areas on lungs.
Animal #4 - yellow areas on intestines; red areas on stomach; mottled liver; dark lungs; mottled and pale kidney, dark and small spleen.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute dermal LD50 was found to be greater than 2000 mg/kg bw.
- Executive summary:
FAT 20003 was tested for acute dermal toxicity potential (LD50) using methodology similar to OECD Guideline 402. The test substance was applied to back of two male and two female rabbits at a dose of 2000 mg/kg bw (1 intact and 1 abraded animal/ sex/ group). The test site was covered with gauze and trunk was wrapped with impervious material for 24 hours. After patch removal, animals were observed for 14 days for mortality, clinical signs and changes in bodyweight. They were submitted at random to a necropsy whenever they died, survivors at the end of the observation period. One animal (#4) with abraded skin was found dead on day 8. No other mortality was observed. Hence, the acute dermal LD50 was found to be greater than 2000 mg/kg bw.
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