Hydrocarbons, C11-14, n-alkanes, isoalkanes, <2% aromatics

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March - November 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

An equilibration study was conducted prior to the study to determine the length of time needed to develop water accommodated fractions (WAFs) at the 1000 mg/L loading level. The aqueous phase of the WAF was analyzed after 1, 24, 48, and 96 hours of stirring. In all samples, the quantity of test substance in solution was less than the limit of detection (0.5 mg/L). Further analysis of test substance in test media a loading level of 1 g/L also showed no detectable amounts of test substance. Consequently, analysis of treatment solutions from the definitive study were not conducted.

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish were obtained from Zeals Trouit Farm, Zeals, Wiltshire, UK, and acclimated to the test conditions for more than nine days before use. A sample of ten of the fish had a mean length of 4.6 cm and a mean weight of 0.97 g.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

242 to 340 mg/L CaCO3

Test temperature:

15 to 17 degrees C

pH:

6.7 to 7.0

Dissolved oxygen:

8.3 to 9.5 mg/L

Nominal and measured concentrations:

The nominal loading rate was 1000 mg/L. A control was also included.

Details on test conditions:

The study was conducted in closed test systems that were renewed daily with fresh treatment solution. The test systems used were 12L glass bottles. Ten fish were exposed to each of the control and treatment solutions and were not fed during the study.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LL0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Ten fish were added to the control and treatment solution. Mortality results are as follows:

Loading Rate Mortality
(mg/L) 24 hr 48 hr 72 hr 96 hr
Control 0 0 0 0
1000 0 0 0 0

Reported statistics and error estimates:

None

Validity criteria fulfilled:

yes

Conclusions:

A water accommodated fraction of the test substance produced a 96-hour LL50 value of >1000 mg/L and a LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in the treatment solution.

Executive summary:

Water accommodated fractions of the test substance produced a 96-hour LL50 value of >1000 mg/L and a LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in the treatment solution.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

The registration substance Hydrocarbons, C11-C14, n-alkanes, isoalkanes, <2% aromatics, EC number 701-280-3 (target) and the test substance Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics (source) are both hydrocarbons with many constituents in common.
The source substance covers the carbon number range of the target substance. The substances therefore have qualitatively similar properties (RAAF Scenario 2 applies).

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Description of key information

Water accommodated fractions of hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 96-hour LL₅₀ value of >1000 mg/L and LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in either the control or treatment test systems. These data are used as read-across data for Hydrocarbons, C11-C14, n-alkanes, isoalkanes, <2% aromatics.

Additional information

No measured fish acute toxicity data are available for Hydrocarbons, C11-C14, n-alkanes, isoalkanes, <2% aromatics, however data are available for a closely related substance, Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics.

Water accommodated fractions of Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 96-hour LL₅₀ value of >1000 mg/L and LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in either the control or treatment test systems. These data are used as read-across data for Hydrocarbons, C11-C14, n-alkanes, isoalkanes, <2% aromatics.