Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-280-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April to May 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted according to a standard guideline without deviations from the protocol, and was conducted under GLP guidelines.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- An un-acclimated activated sludge inoculum was used in this study. The inoculum was obtained from the Chester Sewage Works, Welch Water, Cheshire, UK. The treatment plant receives predominantly domestic sewage. The sludge was collected the day before test initiation. It was prepared for the test by homogenizing it for two minutes after which it was allowed to settle and the supernatant coarse-filtered through medical gauze. The supernatant was added at a 1% loading volume to the test medium and aerated with moist air at the test temperature for approximately 25 hours. Test medium consisted of glass distilled water and mineral salts (phosphate buffer, ferric chloride, magnesium sulfate, calcium chloride). 500 ml of test medium and activated sludge was added to each respirometer flask.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 98 mg/L
- Based on:
- ThOD/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Triplicate test systems were used to evaluate the biodegradability of the test substance and duplicate test systems were used to evaluate the biodegradability of the positive control substance at mean concentrations of 98 and 100 mg/L as ThOD, respectively. Blank test systems, which did not contain the test or positive control substance, were run concurrently in triplicate. The test substance was added to the test systems with a syringe and injected into the test medium below the surface. The test system was sealed immediately after addition of the test substance. An aliquot of the positive control stock solution was added to the appropriate test flasks. All test vessels were stirred constantly using magnetic stir bars and plates. The test systems temperature range was 20.2 – 20.3°C.
- Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- A preliminary study was not conducted.
- Test performance:
- No deviations from the protocol occurred that affected the integrity of the study data.
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 5
- Sampling time:
- 3 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 34
- Sampling time:
- 4 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 50
- Sampling time:
- 15 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 61
- Sampling time:
- 23 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69
- St. dev.:
- 11
- Sampling time:
- 28 d
- Details on results:
- The test substance exhibited rapid biodegradation and was assessed as readily biodegradable. The half-life was approximately 15 days. By Day 28, the average percent biodegradation of the test substance was 69%. The test substance reached 10% biodegradation on approximately Day 4 and 60% biodegradation on approximately Day 23.
Biodegradation was based on oxygen consumption and the theoretical oxygen demand of the test substance was calculated using results of an elemental analysis of the test substance. - Results with reference substance:
- The reference substance biodegraded to an extent of approximately 100% after 28 days. By Day 3, >60% biodegradation of the positive control was observed, which meets the guideline requirement.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- The test substance, a multi-component substance, biodegraded to an extent of 69% after 28 days. The data support characterizing the test substance as rapidly biodegradable, readily biodegradable, not expected to persist in the environment under aerobic conditions. Although it did not meet the 10-day window requirement, it is characterized as readily biodegradable because the criterium is not applied to multi-component substances when assessing their ready biodegradability.
- Executive summary:
The test substance, a multi-component substance, biodegraded to an extent of 69% after 28 days. The data support characterizing the test substance as rapidly biodegradable, readily biodegradable, not expected to persist in the environment under aerobic conditions. Although it did not meet the 10-day window requirement, it is characterized as readily biodegradable because the criterium is not applied to multi-component substances when assessing their ready biodegradability.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- The registration substance Hydrocarbons, C11-C14, n-alkanes, isoalkanes, <2% aromatics, EC number 701-280-3 (target) and the test substance Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics (source) are both hydrocarbons with many constituents in common.
The source substance covers the carbon number range of the target substance. The substances therefore have qualitatively similar properties (RAAF Scenario 2 applies) - Reason / purpose for cross-reference:
- read-across source
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69
- Sampling time:
- 28 d
Referenceopen allclose all
Description of key information
No ready biodegradadation test data are available for the registered substance Hydrocarbons, C11-C14 n-alkanes, isoalkanes, <2% aromatics, however, measured data are available for a substance with the same carbon range as the registered substance.
Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics, biodegraded to an extent of 69% after 28 days. This study is considered to be reliable and the data are used as read-across data for the registered substance, Hydrocarbons, C11-C14 n-alkanes, isoalkanes, <2% aromatics.
Key value for chemical safety assessment
Additional information
No ready biodegradation test data are available for the registered substance Hydrocarbons, C11-C14 n-alkanes, isoalkanes, <2% aromatics, however, measured data are available for a substance with the same carbon range as the registered substance.
Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics biodegraded to an extent of 69% after 28 days in a study conducted according to OECD Technical Guideline 301 F and in compliance with GLP. The data support characterizing Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics as rapidly biodegradable, readily biodegradable, not expected to persist in the environment under aerobic conditions. The 10-day window criterion is not applicable for complex substances where sequential degradation of the constituents takes place (REACH guidance R.7b ECHA, 2017). The substance was therefore concluded to be readily biodegradable. The results from this study are considered to be reliable and the data are read across to the registered substance, Hydrocarbons, C11-C14 n-alkanes, isoalkanes, <2% aromatics.
Supporting data are available for other analogue substances:
Hydrocarbons, C11-C12, n-alkanes, <2% aromatics biodegraded to an extent of approximately 77% after 28 days. The data support characterizing Hydrocarbons, C11-C12, n-alkanes, <2% aromatics as rapidly biodegradable (readily biodegradable).
Hydrocarbons, C13-C14, n-alkanes, <2% aromatics biodegraded to an extent of approximately 79% after 28 days. The data support characterizing Hydrocarbons, C13-C14, n-alkanes, <2% aromatics as rapidly biodegradable (readily biodegradable).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)