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EC number: 800-038-5 | CAS number: 1071838-81-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Lack of analytical determination of the substance
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate is included in the report (date of inspection: from 22 to 24 January 2013)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Stock solutions of CuGUN have been prepared in deionised water acidified with nitric acid to reach pH < 4 in order to stabilized the test item in solution until use. Indeed, according to information provided by the sponsor, CuGUN is rapidly hydrolyzed in N-guanylurea and copper, at pH 7.
Stock solutions were carried out, for each test and used for carrying out the test concentrations.
Stock solution for definitive test (1 g/L): pH: 6.8; conductivity: 567 µS/cm - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The inoculum was prepared from activated sludge sampled in the aeration tank treatment plant receiving predominantly domestic sewage (Montataire - (60) France).
After settling, the supernatant of activated sludge was discarded and the sludge re-suspended in chlorine-free tap water. The wash-water was again removed after settling of sludge and activated sludge were re-suspended in chlorine-free tap water.
The inoculum was continuously aerated and 50 mL of synthetic sewage feed/L activated sludge were added each day until use.
Sampling of the 10th December 2013 and test carried out 11 December 2013: dry matter of inoculum: 3300 mg/L; pH: 7.7; 227.3 mL of inoculum we added per test flask corresponding to a concentration of suspended solids (SS) in the test mixture of 1.5 g/L. - Test type:
- static
- Total exposure duration:
- 3 h
- Post exposure observation period:
- /
- Hardness:
- /
- Test temperature:
- Between 19.5 and 21.0°C
- pH:
- Between 7.0 and 8.0
- Salinity:
- /
- Nominal and measured concentrations:
- Test nominal concentrations: 5.7; 14.8; 38.5; 100 and 260 mg/L
- Details on test conditions:
- Vessel: 1 litre glass beakers
Dilution water: deionised water
Medium: synthetic sewage feed
Total volume of mixtures: 500 mL
Air supply: clean, oil-free. Air flow 0.5 to 1 litre/ min.
Definitive test: Blank controls: 6 test flasks and test concentrations: 4 test flasks for each - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 73.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 23.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 14.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Details on results:
- Statistical tests (Toxstat 3.5, Western Inc. and D. Gulley, University of Wyoming) were performed to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) in the test conditions.
After verification of the normality and homogeneity of variance,Bonferroni test was performed and showed a statistically significant effect from the test concentration of 38.5mg/L.
Therefore, the first test concentration without effect observed is the test concentration 14.8 mg/L of test item.
ECx(3 hours) were calculated using log-logistic regression with bootstrap estimation of the 95% confidence limit:
EC20 3h = 23.4 mg/L (15.5 – 33.9 mg/L)
EC50 3h = 73.8 mg/L (60.6 – 91.3 mg/L)
EC80 3h: Not determinable - Results with reference substance (positive control):
- The EC50 3,5-DCP, after a contact time of 3 hours, was graphically determined between 6.0 and 8.0 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- The blank controls oxygen uptake rate remained above 20 mg O2/g h during the test and the coefficient of variation did not exceed 30% at the end of the test. EC50 (3 hours) of 3,5-dichlorophenol were in the range 2 mg/L and 25 mg/L
- Conclusions:
- In the test conditions, an EC20 of 23.4 mg/L, an EC50 of 73.8 mg/L and a NOEC of 14.8 mg/L have been determined for the test item CuGUN, after a contact time of 3 hours.
- Executive summary:
The total respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed is measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item: CuGUN under otherwise identical conditions is also measured. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean respiration rates of the controls.
In the test conditions, no oxygen consumption was observed for abiotic controls during this range-finding test, showing a lack of reducing properties of the test item and an EC20 of 23.4 mg/L, an EC50 of 73.8 mg/L and a NOEC of 14.8 mg/L have been determined for the test item CuGUN, after a contact time of 3 hours.
.
Reference
Range-finding test: CuGUN
Identification |
Q1 |
Q2 |
Dt |
R |
Rs |
IT |
pH |
|
T0 |
3h |
|||||||
Blank control 1 |
6.9 |
4.2 |
6 |
27.0 |
18.0 |
/ |
7.3 |
7.8 |
Blank control 2 |
6.8 |
4.2 |
6 |
26.0 |
17.3 |
7.3 |
7.7 |
|
Blank control 3 |
6.5 |
3.5 |
6 |
30.0 |
20.0 |
7.3 |
7.7 |
|
Blank controls |
Mean |
27.7 |
18.4 |
/ |
||||
CuGUN 1.0 mg/L |
7.0 |
4.2 |
6 |
28.0 |
18.7 |
- 1.2 |
7.3 |
7.8 |
CuGUN 10.0 mg/L |
7.2 |
4.7 |
6 |
25.0 |
16.7 |
9.6 |
7.2 |
7.8 |
CuGUN 100 mg/L |
8.3 |
7.1 |
6 |
12.0 |
8.0 |
56.6 |
7.0* |
7.9 |
CuGUN 1000 mg/L |
9.3 |
9.2 |
6 |
1.0 |
0.7 |
96.4 |
7.0* |
7.3 |
CuGUN Abio. 1000 mg/L (1) |
9.2 |
9.2 |
6 |
0.0 |
0.0 |
/ |
7.0* |
7.2 |
CuGUN Abio. 1000 mg/L (2) |
9.2 |
9.2 |
6 |
0.0 |
0.0 |
7.1* |
7.2 |
|
CuGUN Abio. 1000 mg/L (3) |
9.3 |
9.3 |
6 |
0.0 |
0.0 |
7.0* |
7.1 |
Specific inhibitor of nitrification: N-allylthiourea (ATU)
Identification |
Q1 |
Q2 |
Dt |
R |
Rs |
IT |
pH |
|
T0 |
T3h |
|||||||
Blank control 1 |
7.4 |
5.3 |
6 |
21.0 |
14.0 |
/ |
7.3 |
8.0 |
Blank control 2 |
7.3 |
5.1 |
6 |
22.0 |
14.7 |
7.3 |
8.0 |
|
Blank controls |
Mean |
21.5 |
14.35 |
/ |
||||
CuGUN 1.0 mg/L |
7.0 |
4.6 |
6 |
24.0 |
16.0 |
- 11.6 |
7.3 |
8.0 |
CuGUN 10.0 mg/L |
6.8 |
4.3 |
6 |
25.0 |
16.7 |
-16.3 |
7.3 |
8.0 |
CuGUN 100 mg/L |
8.0 |
7.1 |
6 |
9.0 |
6.0 |
58.1 |
7.2* |
7.9 |
CuGUN 1000 mg/L |
8.7 |
8.5 |
6 |
2.0 |
1.3 |
90.7 |
7.0* |
7.2 |
*:pH adjustment with NaOH was carried out before addition of the inoculum
Description of key information
The total respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed is measured after a contact time of 3 hours.
The respiration rate of the same activated sludge in the presence of various concentrations of the test item: "CuGUN" under otherwise identical conditions is also measured.
The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean respiration rates of the controls.
An EC50 value is calculated and a no observed effect concentration (NOEC) is statistically determined from the inhibitory effect observed at different concentrations when possible.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 73.8 mg/L
- EC10 or NOEC for microorganisms:
- 14.8 mg/L
Additional information
No oxygen consumption was observed for abiotic controls during the range-finding test, showing a lack of reducing properties of the test item.
Statistical tests (Toxstat 3.5, Western Inc. and D. Gulley, University of Wyoming) were performed to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) in the test conditions. After verification of the normality and homogeneity of variance,Bonferroni test was performed and showed a statistically significant effect from the test concentration of 38.5mg/L.
Therefore, the first test concentration without effect observed is the test concentration 14.8 mg/L of test item.
ECx(3 hours) were calculated using log-logistic regression with bootstrap estimation of the 95% confidence limit:
EC20 3h = 23.4 mg/L (15.5 – 33.9 mg/L)
EC50 3h = 73.8 mg/L (60.6 – 91.3 mg/L)
EC80 3h: Not determinable
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