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EC number: 442-480-8 | CAS number: 182893-11-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Test samples for analysis were taken from the approximate centre of the test vessels according to the following sampling schedule:
- Frequency
At t= 0 h from freshly prepared and at t= 24 h from 24h-old solutions of all test concentrations. At t =72 h from freshly prepared and at t=96 h from 24h-old solutions from 0, 10 and 18 mg/L.
- Volumes
0,10 and 18 mg/I: 6 ml
32 mg/I: 3 ml
56 mg/I: 2 ml
100 mg/I: 1 ml
- Storage
Samples were stored in a deep-freeze until analysis together with freshly taken samples at t=96 hours.
Additionally, reserve samples of 12 ml were taken from all test solutions at t=O and t= 24 hand from the test concentrations with surviving fish at t=72 and t=96 h for possible analysis. If not already used, these samples were stored in a freezer for possible analysis until delivery of the final report with a maximum of three months. - Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with stock solutions at nominally 100 mg/1. These solutions were magnetically stirred for 15 to 20 minutes following treatment with ultrasonic waves for 5 minutes during the range-finding test. The resulting, clear and colourless, stock solutions were then used to prepare the lower test concentrations by subsequent dilutions in test medium. The test solutions were daily renewed during the final test.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- - Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) (Linnaeus, 1758)
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands.
- Mean length: Static range-finding test: 2.5 ± 0.16 cm; Semi-static final test: 2.1 ± 0.18 cm
- Mean weight: Static range-finding test: 0.50 ± 0.12 g; Semi-static final test: 0.13 ± 0.04 g
- Characteristics: F1 from a single parent-pair bred in UV-treated water.
- Feeding: daily with Trouvit - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20.9 - 21.8 °C
- pH:
- 7.4 - 7.8
- Dissolved oxygen:
- 7.5 - 9.0 mg/L
- Nominal and measured concentrations:
- Nominal: 10, 18, 32, 56, 100 mg/L
Measured concentrations were close to nominals, within 80 to 120% of the nominals, therefore nominal concentrations are used. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5.5 L all-glass
- Material, size, headspace, fill volume: 4 L test medium
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.23 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO-medium
- Ca/mg ratio: 20:3
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 0.1, 1.0, 10 AND 100 mg/L
- Results used to determine the conditions for the definitive study: All fish died within 3 hours at 100 mg/L and no fish died in the other test concentrations. - Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I. 14 - 21 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
18 mg/L: 24h and 72-96h swimming at the surface
32 mg/L: 3 3/4h swimming at the surface
56 mg/L: 3 3/4h immobile
- Other biological observations:
10 mg/L: 72-96h discoloured
18 mg/L: 24-96h discoloured
32 mg/L: 3 3/4h discoloured
56 mg/L: 3 3/4h discoloured
- Mortality of control: No mortality
- Other adverse effects control: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Results as expected
- Reported statistics and error estimates:
- the maximum likelihood estimation method with the probits of the percentages of dead fish as function of the logarithms of the corresponding concentrations (Finney, D.J., 1971: Probit analysis, Cambridge University Press, Cambridge, U.K., 3rd edition)
- Sublethal observations / clinical signs:
Conc. (mg/L)
Initial no. fish
Cumulative mortality
Total mortality (%)
3¾h
24h
48h
72h
96h
Control
7
0
0
0
0
0
0
10
7
0
0
0
0
0
0
18
7
0
1
5
5
5
71
32
7
0
7
7
7
7
100
56
7
2
7
7
7
7
100
100
7
7
7
7
7
7
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h-LC50 was already reached within 48 hours of exposure being 16 mg/l with a 95% confidence interval between 14 and 21 mg/l.
- Executive summary:
96-Hour Acute Toxicity Study in carp.
The study procedures described in this report were based on the EEC directive 92/69; Part C: methods for the determination of ecotoxicity, Publication No. L383, December 1992, C.1 . "Acute toxicity for fish", and the OECD guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992.
The test substance was completely miscible with test medium at the concentrations tested.
The project started with a static range-finding test exposing three carp per test group to nominal test substance concentrations of 0.1, 1.0, 10 and 100 mg/l. All fish exposed to nominally 100 mg/l died within 3 hours of exposure. No mortality was observed at the lower test concentrations. Hence, the 96h-LC50 for carp was expected to be between 10 and 100 mg/l. Analytical results showed that the measured concentrations of both main components decreased by more than 20% during the test period. It was decided to continue testing applying a semi-static test design with daily renewal of test solutions as concentrations did not decrease by more than 20% during the first 24-hour test period.
The project was continued with a final LC50 study exposing seven carp per concentration to test substance concentrations ranging from 10 to 100 mg/l, increasing with a factor of 1.8. All test solutions were daily renewed. Samples for analysis were taken at the start and after 72 hours of exposure from freshly prepared solutions and from 24-hour old test solutions at 24 and 96 hours of exposure.
Analysis of the samples taken during the final test showed that the measured concentrations (based on both components) were in agreement with nominal in the freshly prepared solutions at the start of exposure (88-101 %) and the freshly prepared solutions at 72 hours of exposure (93-99%). This indicated that preparation procedures were adequate and repeatable. During the 24-hour periods between renewals the concentrations measured did not decrease by more than 20% below initial. In addition, the average exposure concentrations all remained above 80% relative to nominal. Consequently, the calculated toxicity parameters were based on the nominal test concentrations. In the control group no fish died, and all test conditions (pH, oxygen and temperature) remained within the ranges prescribed by the protocol.
The test substance induced no mortality in carp at or below nominally 10 mg/l.
The 3%h-LC50 was 60 mg/l with a 95% confidence interval between 52 and 81 mg/l.
The 24h-LC50 was estimated to be 21 mg/l with 14% mortality at 18 mg/l and 100% mortality at 32 mg/l (regression line: log10(conc.)=1.01 +(probit-2.61 )/7.73).
The 96h-LC50 was already reached within 48 hours of exposure being 16 mg/l with a 95% confidence interval between 14 and 21 mg/l.
Reference
Description of key information
The 96h-LC50 was already reached within 48 hours of exposure being 16 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 16 mg/L
Additional information
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