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EC number: 309-916-8 | CAS number: 101357-19-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material : Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates
- Molecular formula :C8H22NO12P.Mo.W
- Molecular weight : 635.02 g/mol
- Smiles notation : CN(C)c1ccccc1_O=[Mo](=O)(O{-})O{-}_O=[W](=O)(O{-})O{-}_OP(O)(O)=O
- Substance type: Organic
- Physical state : Solid - Analytical monitoring:
- yes
- Vehicle:
- not specified
- Details on test solutions:
- The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively.
The test substance was stable in RO water. In house water solubility was 0.2308 mg/L in milliQ water hence stock prepared in RO water with 4g/4L by giving 24 hrs stirring, after stirring stock was analytically detected - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Anytime Pet,Jaripatka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.7 cm
- Weight at study initiation (mean and range, SD): average 0.066 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod:16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 153.5 mg of CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.1 mg/l
- Nominal and measured concentrations:
- 0.0459 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- < 0.046 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Mortality were observed
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 0.046 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 100 % mortality were observed
- Sublethal observations / clinical signs:
8.1. Mortality and symptoms
Nominal
Number of
Mortality (cumulated) after
Concentration fish
3 h
6 h
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.0459
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
symptom (cumulated) after
Nominal
Number of
3 h
6 h
24h
48h
72h
96h
Concentration
fish
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1 V2
(mg/l)
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.0459
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Number of
pH readings after
Nominal
fish
24h
48h
72h
96h
Concentration
V1
V2
V1
V2
V1
V2
V1
V2
(mg/l)
Control
4
4
-
-
-
-
-
-
-
-
0.0459
4
4
-
-
-
-
-
-
-
-
Nominal
Number of
Oxygen content (mg/L) after
Concentrationfish
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
0.0459
4
4
-
-
-
-
-
-
-
-
8.3. Test temperature
Nominal
Number of
Temperature (°C) after
Concentrationfish
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
0.0459
4
4
-
-
-
-
-
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on Zebra Fish Danio rerio was determine to be < 0.0459 mg/l.
- Executive summary:
Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.
Reference
Description of key information
Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.046 mg/L
Additional information
Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.
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