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EC number: 231-967-9 | CAS number: 7782-87-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-07-27 till 2009-07-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study in compliance to GLP standards.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- only one concentration was tested (limit test 1000 mg/L) Water solubility of the test substance is at least 5g/L
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- in stead of a minimum of five concentrations, only one concentration was tested (limit test 1000 mg/L) Water solubility of the test substance is at least 5g/L
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The aerobic activated sludge from the wastewater treatment plant (ARA Ergolz II, Füllinsdorf / Switzerland) was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. Based on this ratio, an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter.
- Pretreatment: Three days prior to use, the sludge was fed daily with 50 mL synthetic sewage feed per liter (composition is given in "other information on materials and methods") and was kept at room temperature under continuous aeration until use.
- Initial biomass concentration: 3.3 g/L dry weight
- pH of the activated sludge inoculum: 7.8 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 21°C
- pH:
- 7.3 - 8.3
- Dissolved oxygen:
- 8.1 - 9.4 mg 02/L
- Nominal and measured concentrations:
- Nominal concentration test substance: 1000 mg/L
Nominal concentration reference substance: 5, 16 and 50 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material: 2000 mL glass beakers
- Aeration: continuously by intense stirring on magnetic stirrers to avoid possible foaming and/or stripping of the test item
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: about 1.3 g dry material per liter test medium
TEST MEDIUM
- Preparation:
500.1 mg of the test substance was dissolved in 284 mL tap water by intense stirring for 5 minutes at room temperature. Afterwards 16 mL synthetic sewage feed and 200 mL activated sludge inoculum were added.
- Composition of test medium: as recommended in the guideline
EFFECT PARAMETERS MEASURED: oxygen consumption rate
- Methodology of measurement:
A well-mixed sample of each test medium was poured into a BOD-flask after incubation and was not further aerated. Then the dissolved oxygen concentration was measured and continuously recorded. During these measurements, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption rate (in mg O2 L-1 minute-1) was determined from the linear part of the respiration curve in the range 6.5–2.5 mg O2/L. In case of very rapid oxygen consumption, the range used was below the limits indicated previously but always within the linear part of the respiration curve. In case of low oxygen consumption the rate was determined over a period of at least ten minutes.
- Measurement equipment: oxygen electrode (WTW TriOxmatic® 300) and an oxygen meter (WTW Oxi 539, Wissenschaftlich-Technische Werkstaetten WTW, Weilheim/Germany)
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: based on EU testing guidelines A limit test was performed to demonstrate that the test item has no toxic effect on activated sludge up to the nominal test concentration of 1000 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no significant inhibitory effect
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: absence of toxic effect with limit test
- Details on results:
- The EC20, EC50 and EC80 (3 hours) could not be calculated but were clearly higher than 1000 mg/L
- Results with reference substance (positive control):
- - Results with reference substance are valid
- Relevant effect levels: EC50 (3h): 18 mg/L (95% confidence limits could not be calculated due to mathematical reasons) - Reported statistics and error estimates:
- - Test substance: 3h EC20, EC50 and EC80 and their 95%-confidence limits could not be calculated because of the absence of a toxic effect
- Reference item: 3h EC50 was calculated by Probit Analysis - Validity criteria fulfilled:
- yes
- Remarks:
- see section overall remarks, attachments
- Conclusions:
- In the test conditions (limit test at 1000 mg/L) no toxic effects were observed. Thus the test substance has no significant inhibitory effect on the respiration rate of activated sludge.
- Executive summary:
The authors tested the respiratory inhibition potential of sodium hypophosphite (CAS n° 10039-56-2) to activated sludge according to the EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test) and to the OECD guideline 209 (Activated Sludge, Respiration Inhibition Test). A 3h respiration inhibition limit test was performed with a nominal concentration of 1000 mg/L. In addition, two controls (at the start and end of the test) were tested in parallel with the test substance. Furthermore, in parellel 3,5-dichlorophenol (positive control) was tested at concentrations of 5, 16, and 50 mg/L.
Under these conditions no significant toxic effects were observed for the test substance and the results of the positive control confirmed suitability of the activated sludge and the method used. Hence, the EC10 (3h) of the test substance to activated sludge microorganisms was at least 1000 mg/L. The EC20, EC50, and EC80 (3h) could not be calculated but were clearly higher than 1000 mg/L.
Therefore, in the test conditions the test substance has no significant inhibitory effect on the respiration rate of activated sludge.
This study was performed according to OECD guidelines, in compliance to GLP standards (certificate included) and all validity criteria are fulfilled Hence the study can be consided as klimisch 1: reliable without restrictions.
Reference
- Table 1: Detailed overview of the results obtained during the test as well as the influence of te test substance sodium hypophosphite and 3,the reference substance 5-Dichlorophenol on the oxygen consumption of activated sludge.
Vessel no. |
Nominal concentration of test chemical (mg O2/L) |
Oxygen consumption rate (mg O2L-1min-1) |
Inhibition (%) |
pH |
Oxygen concentration (mg/L) |
||
start* |
end* |
start* |
end* |
||||
Control A |
0 |
1.505 |
|
7.6 |
8.3 |
8.6 |
8.5 |
Control B |
0 |
1.333 |
|
7.7 |
8.2 |
8.5 |
8.8 |
Mean |
1.419 |
|
|||||
Deviation (%) |
11.5 |
|
|||||
|
|||||||
Test item |
1000 |
1.336 |
5.9 |
7.3 |
8.2 |
8.2 |
9.1 |
|
|||||||
Reference item 1 |
5 |
1.553 |
-9.5 |
7.6 |
8.2 |
8.1 |
8.6 |
Reference item 2 |
16 |
0.770 |
45.7 |
7.6 |
8.3 |
8.6 |
8.8 |
Reference item 3 |
50 |
0.172 |
87.9 |
7.6 |
8.3 |
8.6 |
9.4 |
* Start and end of the 3h incubation period
- Controls: oxygen consumption rates of the two controls (run at the start and at the end of the test) differed by 12%.
Description of key information
Toxicity of sodium phosphinate to activated sludge microorganisms:
- EC10 (3h) >= 1000 mg/L (EU Method C.11 and OECD guideline 209)
- EC50 (3h) > 1000 mg/L (EU Method C.11 and OECD guideline 209)
By read-across, these results also apply to potassium phosphinate.
Key value for chemical safety assessment
Additional information
A 3h respiration inhibition limit test was performed with a nominal concentration of 1000 mg/L of sodium phosphinate according to the EU Method C.11 and the OECD guideline 209 (Eisner, 2009).Two controls and a positive control (3,5 -dichlorophenol) were tested in parallel.
No significant toxic effects were observed and the results of the positive control confirmed suitability of the activated sludge and the method used. The EC10 (3h) of the test substance to activated sludge microorganisms was at least 1000 mg/L. The EC20, EC50, and EC80 (3h) could not be calculated but were clearly higher than 1000 mg/L. Therefore, sodium phosphinate has no significant inhibitory effect on the respiration rate of activated sludge and is thus not toxic to these microorganisms. By read-across, potassium phosphinate does not have any inhibitory effect either.
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