N,N-dibutyloleamide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N,N-dibutyloleamide

EC Number:

227-410-4

EC Name:

N,N-dibutyloleamide

Cas Number:

5831-80-1

Molecular formula:

C26H51NO

IUPAC Name:

N,N-Dibutyloleamide

Test material form:

other: yellow liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

18 - 22 °C

pH:

7,2

Salinity:

freshwater

Nominal and measured concentrations:

100 - 320 - 1000 - 3200 - 10000 mg/L

Reference substance (positive control):

yes

Remarks:

copper (II) sulphate pentahydrate

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50 = 93 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at concentrations up to 10000 mg/L.

Executive summary:

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item.Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the concentration 100 – 320 – 1000 – 3200 - 10000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates.

 

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined.

 

EC-Values of the test item

		EC-values [mg/L]
EC20		> 10000
EC50		> 10000
EC80		> 10000

 

The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at concentrations up to 10000 mg/L.