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Description of key information

The study documentation of the available skin irritations studies is incomplete in one case and in the two other cases the test substance was applied undissolved or respectively diluted. However, the studies gave no evidence of a skin irritating potential of 1-chloro-2-nitrobenzene.
1-Chloro-2-nitrobenzene caused slight irritational effects to the eyes of rabbits which were reversible within 24 hours.

Key value for chemical safety assessment

Additional information

Skin Irritation

There are no studies according to the current OECD guideline but there are study reports with rabbits which give sufficient information to evaluate this endpoint:

In an older study, 0.5 mL of a 10 % sesame oil solution of 1-chloro-2-nitrobenzene was applied to the shaved (intact and abraded) skin of six rabbits for 24 hours covered by semi-occlusive dressing. When the dressing was removed (24 hour-reading) only mild erythema (score 1/0-4) was noted in both, intact and abraded skin of 4/6 rabbits. Erythema was not observed at 48 hour- and at 72 hour-reading. According to Fed. Reg. 38, No 187, p. 27109, §1500.41, 1973, the compound was evaluated as no irritant (Hoechst 1975).

In another study, 500 mg 1-chloro-2-nitrobenzene was applied undissolved to the inner surface of one ear of each of two rabbits for 24 hours covered by cellulose pads and plaster. To fix the plaster tightly a rolled gauze pad was put on it. Ear, substance, pad, plaster and rolled pad were then bandaged. No signs of irritation (sore 0/4) were observed neither when the pad, plaster, rolled pad were removed nor during the 7 day post exposure observation period (Bayer 1976). In addition, in the same report, the results of acute dermal testing in rats with the substance formulated in polyethylene glycole 400 are mentioned. Signs of irritation were not reported. 0.5 mL of warmed, undiluted 1-chloro-2-nitrobenzene was applied to the skin of six rabbits for 24 hours. No erythema or edema was observed till 168 hours after application (no information about the type of application and pretreatment of the skin) (Younger Labs. 1991).

No skin irritation was reported in an acute dermal toxicity study (Bayer 1976).

 

Eye Irritation

There are no studies according to the current OECD guideline but there are study reports with rabbits which give sufficient information to evaluate this endpoint:

In an older study, performed as described in Fed. Reg, Vol. 38, No.187, §1500.42, 1973, 100 mg of 1-chloro-2-nitrobenzene was applied undissolved into one eye of each of 6 rabbits (the other eye served as control). One hour post application slight conjunctival injections (score 1-2/0-3) were noted in the eyes of 6/6 rabbits, 7 hours post application in the eyes of only 2/6 rabbits (score 1/0-3) and 24 hours post application no irritational effects were observed. The compound was evaluated to be a mild irritant (Hoechst 1975).

In another study in the same report, a 10 % solution was applied into one eye of each of 6 rabbits which leads to slight irritational effects (score 1/0-3) in the eyes of 3/6 rabbits one hour post application. These effects had disappeared after 7 hours. The compound was evaluated as slightly irritating (Hoechst 1975).

In another study 50 mg 1-chloro-2-nitrobenzene was applied into the right eye of each of two rabbits. Slight redness (score 1/3) was observed in the eye of one rabbit, which disappeared within 24 hours. No signs of irritation were observed in cornea neither on the application day nor during the 7 day post exposure observation period (Bayer 1976).

Justification for classification or non-classification

Based on the available data, the substance does need not to be classified for skin or eye irritation according to Directive 67/548/EEC and according EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.