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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No pathologic examination performed, individual animal data and information on GLP are missing.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976
Reference Type:
other: OECD SIDS
Title:
1-Chloro-2-nitrobenzene, CAS: 88-73-3
Author:
OECD SIDS
Year:
2001
Bibliographic source:
SIDS Initial Assessment Report for SIAM 13

Materials and methods

Principles of method if other than guideline:
10 or 20 rats/dose, 6 (males) or 3 (females) doses, substance (dissolved in polyethylene glycol 400) applied on the shaved back for 24 hours, covered by aluminium foil and a plaster, then rinsed with water and soap, post exposure observation time: 14 days.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: polyethylene glycol 400
Details on dermal exposure:
TEST SITE
- Shaved back
- Covered by aluminium foil and a plaster
REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water and soap
- Time after start of exposure: 24 hours



Duration of exposure:
24 hours
Doses:
Male rats: 250, 350, 500, 750, 1000, 1500 mg/kg bw
Female rats: 750, 1000, 1500 mg/kg bw
No. of animals per sex per dose:
Male rats: 10 per dose
Female rats: 10 per dose at 750 and 1500 mg/kg bw, 20 at 1000 mg/kg bw
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
655 mg/kg bw
Based on:
test mat.
Sex:
female
Dose descriptor:
LD50
Effect level:
1 320 mg/kg bw
Based on:
test mat.
Mortality:
Male rats:
1, 1, 3, 7, 7, and 9 rats died at 250, 350, 500, 750, 1000 and 1500 mg/kg bw, respectively.
Time of death: between 1 and 4 days after initiation of treatment.
Female rats:
0, 5 and 6 rats died at 750, 1000 and 1500 mg/kg bw, respectively.
Time of death: between 2 and 6 days after initiation of treatment.
Clinical signs:
Reduced general condition, difficulties in breathing, cyanotic appearance and, in some animals, lacrimation. The signs of intoxication started 18 hours post application and occurred at all doses in both sexes.

Applicant's summary and conclusion