Registration Dossier

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Gross autopsy not reported, no information about GLP.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1992
Reference Type:
other: OECD SIDS
Title:
1-Chloro-2-nitrobenzene, CAS: 88-73-3
Author:
OECD SIDS
Year:
2001
Bibliographic source:
SIDS Initial Assessment Report for SIAM 13

Materials and methods

Principles of method if other than guideline:
10 male rats/concentration, head-only exposure, 6 concentrations, heated vapour was diluted with humidified and O2-enriched air and thus converted to a mixture of vapour and liquid aerosol, post exposure observation time: 14 days
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity 99.8%

Test animals

Species:
rat
Strain:
other: CD
Sex:
male

Administration / exposure

Route of administration:
other: inhalation: mixture of vapour and liquid aerosol
Type of inhalation exposure:
head only
Vehicle:
other: humidified and O2-enriched air
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
1.56, 1.83, 2.46, 2.64, 3.23, 3.33 mg/L
No. of animals per sex per dose:
10 male rats/concentration
Control animals:
no
Statistics:
LC50 value was calculated from statistically not significant regression (because mortality was not strictly dose-dependant).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 3.2 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Mortality:
1.56 mg/L: 1 rat (day 5)
1.83 mg/L: 3 rats (2 on day 2, 1 on day 3)
2.46 mg/L: 2 rats (days 2 and 3)
2.64 mg/L: 10 rats (1 on day 0, 1 on day 1, 7 on day 2, 1 on day 3)
3.23 mg/L: 1 rat (day 2)
3.33 mg/L: 6 rats ( 1 on day 2, 2 on day 2, 2 on day 3, 1 on day 7)
Clinical signs:
During exposure: slight to moderate cyanosis, semi-prostration, lethargy and reddish brown nasal discharge to 24 hours, slight to moderate corneal opacity, tachypnea, some rats with partial hind-leg paralysis, abnormal arched-back posture.
Post exposure: pallor, stained perineal area, lethargy, some rats with salivation, lacrimation and corneal opacity, chromodacryorrhea.
Body weight:
Weight loss of 8 to 16% from 1 to 3 days with normal gains thereafter.

Applicant's summary and conclusion