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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Remarks:
but main study waived
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Silicic acid, calcium salt
EC Number:
215-710-8
EC Name:
Silicic acid, calcium salt
Cas Number:
1344-95-2
IUPAC Name:
Silicic acid, Calcium salt
Test material form:
solid
Specific details on test material used for the study:
Name of test material (as cited in study report) = Shieldex AC 3

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Aqueous extracts of loadings (water-accommodated fraction, WAF)
after 24-h shaking of the suspensions in deionised water at approx. 130 revolutions/min;.
fitration through folded paperfilter;
addition of necessary salts from stock solutions before test.
- Differential loading: yes
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Filtrates (WAFs) were clear.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus
- Source: Dr. Knie LWA
- Age at study initiation (mean and range, SD): >= 6 h; =< 24 h
- Method of breeding: daily in drinking water at 20 +-1 °C


ACCLIMATION
- Type and amount of food: Scenedesmus subspicatus
- Feeding frequency: daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
no data
Test temperature:
20 ± 1 °C
pH:
control: 7.86 and 7.66
tests: 7.81 - 9.35
Dissolved oxygen:
8.0 - 8.7
Salinity:
no data
Nominal and measured concentrations:
100; 1000; and 10,000 mg/L (loading)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: no data
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 dark
- Light intensity: no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 and 48 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: 100; 1000; and 10000 mg/L
- Results used to determine the conditions for the definitive study: no main study performed
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EL0
Remarks:
loading without effect
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No toxic effects observed.
Reported statistics and error estimates:
not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:
yes