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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Remarks:
but main study waived
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Specific details on test material used for the study:
Name of test material (as cited in study report) = Shieldex AC 3

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Aqueous extracts of loadings (water-accommodated fraction, WAF)
after 24-h shaking of the suspensions in deionised water at approx. 130 revolutions/min;.
fitration through folded paperfilter;
addition of necessary salts from stock solutions before test.
- Differential loading: yes
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Filtrates (WAFs) were clear.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus
- Source: Dr. Knie LWA
- Age at study initiation (mean and range, SD): >= 6 h; =< 24 h
- Method of breeding: daily in drinking water at 20 +-1 °C


ACCLIMATION
- Type and amount of food: Scenedesmus subspicatus
- Feeding frequency: daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
no data
Test temperature:
20 ± 1 °C
pH:
control: 7.86 and 7.66
tests: 7.81 - 9.35
Dissolved oxygen:
8.0 - 8.7
Salinity:
no data
Nominal and measured concentrations:
100; 1000; and 10,000 mg/L (loading)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: no data
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 dark
- Light intensity: no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 and 48 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: 100; 1000; and 10000 mg/L
- Results used to determine the conditions for the definitive study: no main study performed
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EL0
Remarks:
loading without effect
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No toxic effects observed.
Reported statistics and error estimates:
not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:
yes