acetalization product between glucose and C14 alcohol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2006, November the 21st to december the 1st

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: No amendment to the study plan was requested. No incident, which could have affected the quality or the interpretation of the results obtained, was observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

2007-01-12

Test material

Test material information

Constituent 1

Reference substance name:

Montanov 14

IUPAC Name:

Montanov 14

Details on test material:

- Name: Montanov 14
- Batch n°: T53815
- Appearence: pearls of was
- Purity: ES>99%
- Stability: water 96h
- Solubility: water<0.1g/L
- Expiration date: 22.09.07
- Storage: romm temperature 20 +/- 5°C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

As the solubility lies below 100mg/L, the water-accomodated fraction was prepared for the test. this was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours; the resulting solution was filtrated through 0.45 um filters.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Variety STRAUS
Strain: Berlin
Sex: female
Age: between 0 end 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: October 4th 2005
Selection of the test system was made following the proposal of the guidelines.
Food: unicellular green algae, not during the test
Medium renewal: twice a week
Photo period: 16/8 hours, using neon tubes
T: 20 +/- 2°C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.50 mmol/L

Test temperature:

21.4-22.4°C

pH:

from 7.4 to 8

Dissolved oxygen:

above 8.3 mg/L

Details on test conditions:

- Vessels: glass beakers, nominal volume 50 mL, tall shape
- Feeding: none
- lighting: none
-Temperature: 20 +/-2°C

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

24h EC50 of K2Cr2O7 was determined at 1.4mg/L
Immobilisation in the controls was 10% at he most
The lowest concentration of dissolved oxygen at the end of the test was 8.3mg/L
The highest pH variation was 0.4 units.
The study can be considered valid

Results with reference substance (positive control):

24h EC50 of K2Cr2O7 was determined at 1.4mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

24h NOEC= 100mg/L
48h NOEC= 100mg/L
24h EC50i> 100mg/L
48h EC50> 100 mg/L

Executive summary:

This study was performed in order to evaluate the toxic potential of MONTANOV 14 towards freshwater shrimp, using species Daphnia magna. D magna STRAUSS, which belongs to the family of crustacea, was chosen in the guideline as a typical part of zoo-plancton.

As the solubility lies below 100mg/L, the water-accomodated fraction was prepared for the test. t his was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours; the resulting solution was filtrated through 0.45 um filters.

In the main study, 3 concentrations of the test item was used, spaces by factor 10, the highesty being 100 mg/L were used. 20 Daphnia were exposed to the test item for 48 hours, immobilised Daphnia were counted.

24h EC50 of K2Cr2O7 was determined at 1.4mg/L, lying with demanded range of 0.6 -2.1mg/L.

The following results were obtained for the test item:

24h NOEC= 100mg/L

48h NOEC= 100mg/L

24h EC50i> 100mg/L

48h EC50> 100 mg/L