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EC number: 423-300-7 | CAS number: 128554-52-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-03-01 to 2007-06-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Validity criteria of guideline are fulfilled.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 423-300-7
- EC Name:
- -
- Cas Number:
- 128554-52-9
- Molecular formula:
- Not available
- IUPAC Name:
- Reaction products of 12-hydroxyoctadecanoic acid and octadecanoic acid and 1,3-phenylenedimethanamine
- Details on test material:
- - Name of test material (as cited in study report): WAX#700
- Lot/batch No.: 001
- Stability under test conditions: After the exposure period, infrared absorption spectrum of the test substance was measured.
The spectrum was consistent with that measured before the exposure period, indicating that the test substance was stable under the storage
conditions.
- Storage condition of test material: in a dessiccator at room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Activated sludge from domestic sewage treatment plant
Naruse Clean Center, Machida city, Tokyo, Japan
- Concentration of sludge: Mixed liquor suspended solid (MLSS) : 4890 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- 100 mg/L
- Based on:
- other: reference substance (aniline)
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- other: BOD
- Details on study design:
- TEST SYSTEM
Test bottle contents :
Bottle 1 : Aniline + activated sludge + mineral medium
29 µL (30 mg) of aniline was added to the mineral medium , then activated sludge was added.
Bottle 2: Activated sludge + mineral medium
Activated sludge was added to the mineral medium' .
Bottles 3, 4, 5: Test substance + activated sludge + mineral medium
30 mg of the test substance was added to the mineral medium·, then activated sludge was added.
Bottle 6: Test substance + purified water
30 mg of the test substance was added to 300 mL of purified water
The total volume of the mineral medium and the sludge was held fixed at 300 mL,
resulting in the concentration of test substnce and reference substance 100 mg/L.
Reference substance
- Reference substance:
- aniline
Results and discussion
- Test performance:
- Validity
The biodegradability test was judged valid because the measurement results fulfilled the validity criteria of the guidelines:
1) The degradability based on BOD measurement of reference substance (aniline) is greater than 60% at day 14.
2) The difference of extremes of replicate values of degradability at day 28 is less than 20%.
3) BOD in the blank (bottle 2) is less than 60 mg 021L after day 28.
% Degradation
- Parameter:
- other: BOD
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottles 3, 4 and 5
- Details on results:
- Degradability based on BOD
At day 28, BOD in bottles 3, 4 and 5 were 0.02, 0.04 and 0.02 mg02/mg, respectively. ( Maximum theoretical value: 2.97 mg02/mg)
The degradability were all 1% for bottles 3, 4 and 5.
Degradability based on DOC
DOC in bottles 3, 4 and 5 (as corrected for the value in bottle 2) were 0.0, 0.1 and -0.4 mg, respectively. DOC in bottle 6 was 0.4 mg.
(* Maximum theoretical value: 22.8 mg)
Degradability was not calculated because the test substance was insoluble in mineral medium and DOC in bottle 6 was less than 90% of the theoretical value.
BOD5 / COD results
- Results with reference substance:
- The degradability based on BOD measurement of reference substance (aniline) is greater than 60% at day 14.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
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