[1,1'-Bianthracene]-9,9',10,10'-tetrone, 4,4'-bis[[4,6-bis[[3-(diethylamino)propyl]amino]-1,3,5-triazin-2-yl]amino]-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan 2014 - Feb 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

308 AQN

IUPAC Name:

308 AQN

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): 308 AQN

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): Poorly water soluble

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 1000 mg/L

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Desmodesmus subspicatus CHODAT was used as the test system (Origin: Sammlung von
Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen [SAG], Strain-No. 86.81).
The algae were cultivated under aseptic / sterile conditions.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

not performed

Test conditions

Test temperature:

24+/- 1°C

pH:

8.13 (t0) - 9.36 (72h)

Nominal and measured concentrations:

The reported effect concentrations (EL values) refer to nominal concentrations of the test item.

Details on test conditions:

Three days before starting of the test (main test), an algal pre-culture was made which was
inoculated from an algal stock culture derived from the algal type culture collection Göttingen in
order to guarantee sterile conditions at all. The pre-culture was incubated under the same
conditions as the test solutions to be tested afterwards.
At t0 the test solutions were prepared. Absorbance values of the solutions were determined at t0
(theoretical value on basis of the measured value for the inoculum), after 25 hours, 49 hours and
72 hours using a spectrophotometer at 578 nm.
The incubation of the test and control solutions took place according to the OECD Guideline 201
for a period of 72 hours at a temperature of ≈23.5°C at an illumination rate of >120
μE/m2s ([8000 Lux] measured once per month with a spherical collector within the range of 400-
700 nm). In order to assure the input of CO2, the test solutions were stirred by means of a
magnetic stirrer for the duration of 15 minutes per hour. Longer stirring periods were avoided to
prevent damage of the algal cells.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

- Exponential growth in the control (for algal test): yes

Reported statistics and error estimates:

According to OECD 201, the factor of the biomass parameter, measured in the control between 0 and 72 h,
must be at least 16. With the current test it was found to be 189,0. The test fulfills this validity criterion.
The mean of the replicate coefficients of variation in the section-by-section growth rate was: 15,2%. According to OECD 201, the mean coefficient of variation, measured in the control from 0 to 72 h, must not be higher than 35%. The test fulfills this validity criterion.
The coefficient of variation of the mean specific growth rate replicates in the control between 0 and 72 h was: 1,3%.
According to OECD 201, the coefficient of variation of the mean specific growth rate, measured in the control
from 0 to 72 h, must not exceed 7%. The test fulfills this validity criterion.

Any other information on results incl. tables

Yield (0 - 72 h): EL 50 >1000 mg/L, Section-by-section growth rate (47-72h): EL 50 >1000 mg/L, Growth rate (0 - 72 h):

EL 50 >1000 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EL 50 of 308 AQN towards algae is found to be > 1000 mg/L.

Executive summary:

The aim of this study was the determination of the chronic toxicity towards algae of “308 AQN” according to OECD-Guideline No. 201, in the Version dated 23-March-2006.

The toxic behaviour was investigated by determination of the inhibition of the growth rate of the algae and the yield during the exposure period of 72 hours. As the test item exhibits an extreme poor water solubility it was tested using aqueous extracts (“WAFs”) at nominal concentrations up to and including 1000 mg test item/L (final concentration). The main test was performed as a limit test using “WAF” with nominal concentation from 1000 mg/L of the test item. The following EL-values (“effective loading”) with 308 AQN are assumed:

Yield (0 - 72 h): EL 50 >1000 mg/L, Section-by-section growth rate (47-72h): EL 50 >1000 mg/L, Growth rate (0 - 72 h):

EL 50 >1000 mg/L.

Supporting chemical analyses were not performed within this study as the test item could not be determined by any analytical method because of the huge molecular weight of the molecule. Based on this, the reported effect concentrations (EL values) should refer to nominal concentrations of the test item. The validity criteria for the study as given by the OECD Guideline 201 were met and the study is therefore considered to be valid.