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EC number: 908-343-6
CAS number: -
Table 1: Results of the second cytogenicity study.
MPE/1000 PE b)
PE/NE ration c)
Time of sacrifice d) (hours)
a) 10 animals (5 males, 5 females) per group; b) based on scoring of
2000 polychromatic erythrocytes; c) based on a total of 1000
erythrocytes (PE + NE); d) time following treatment (intraperitoneal
injection, dissolved in water); MPE: micronucleated polychromatic
erythrocytes; PE: polychromatic erythrocytes; NE: normochromatic
erythrocytes; SD: standard deviation; * p < 0.05, ** p < 0.01, *** p <
0.001 (X-square test for MPE, Student's t-test for PE/NE ratio)
Table 2: Clinical signs and mortality noted in the frist cytogenicity
Males (animals/total number of animals)
Females (animals/total number of animals)
2 h – 48 h
24 h – 48 h
Convulsions before death
Convulsions, piloerection, hypoactivity
* Animals remaining after first intraperitoneal injection; ** Animals
remaining after second intraperitoneal injection
Table 3: Clinical signs and mortality noted in second cytogenic study.
Solution at 4 % hydrogen peroxide
The potential of hydrogen peroxide to induce cytogenetic damage to the
bone marrow cells of Swiss OF1 mice was tested in a micronucleus assay
in accordance with OECD Guideline No. 474 and under GLP conditions.
Preliminary toxicity tests were performed to define the doses to be used
in the cytogenetic test. Six groups of 5 male and 5 female mice received
the test substance by a single intraperitoneal injection at doses of
250, 500 and 1000 mg/kg. Two groups of 5 males and 5 females received
the vehicle (water) alone. One group of 5 males and 5 females was
treated with the positive control substance cyclophosphamide
administered by a single oral dose of 50 mg/kg. For each animal, bone
marrow cell smears were prepared and the micronuclei were counted in
2000 polychromatic erythrocytes. The polychromatic (PE) to
normochromatic (NE) erythrocyte ratio was established by scoring 1000
erythrocytes (PE + NE). In the two vehicle control groups, the mean
values of micronucleated polychromatic erythrocytes (MPE) were in the
range of historical controls. Cyclophosphamide induced a highly
significant increase in the number of MPE and significantly decreased
the PE/NE ratio, indicating the cytotoxicity of the control substance.
In all groups treated with hydrogen peroxide, the mean values of MPE
were similar to those of their respective vehicle controls. A slight
statistically significant increase in the MPE number in the low-dose
group after 24 hours was considered as biologically insignificant. A
statistically significant decrease in the PE/NE ratio in most treated
groups after 24 and 48 hours showed that hydrogen peroxide effectively
affected the bone marrow cells. It was concluded from the findings that
under the experimental conditions hydrogen peroxide did not induce
cytogenetic damage in bone marrow cells of mice when administered by the
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