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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
yes
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-p-phenylenedioxydiethanol
EC Number:
203-197-3
EC Name:
2,2'-p-phenylenedioxydiethanol
Cas Number:
104-38-1
Molecular formula:
C10H14O4
IUPAC Name:
2,2'-[1,4-phenylenebis(oxy)]diethanol

Test animals

Species:
mouse
Strain:
CD-1
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
0.5% CMC
Duration of treatment / exposure:
Oral gavage dose
Frequency of treatment:
Twice, at 0 and 24 hours
Post exposure period:
24 hours after second dose
Doses / concentrations
Remarks:
Doses / Concentrations:
2000 mg/kg
Basis:
actual ingested
No. of animals per sex per dose:
10 males and 10 females
Control animals:
yes, concurrent vehicle
Positive control(s):
50 mg/kg Cyclophosphamide

Examinations

Tissues and cell types examined:
Bone marrow from one femur

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Positive controls validity:
valid

Any other information on results incl. tables

No deaths or adverse reactions were observed following dosing.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: negative
HQEE was considered to be non-mutagenic in an vivo micronucleus assay.