Ethanaminium, 2-hydroxy-N-(2-hydroxyethyl)-N,N-dimethyl-, esters with C16-18 and C18-unsatd. fatty acids, chlorides

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug 16, 1985 - Sept 13, 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

no analytical monitoring, 24 hr study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

(24 h study, no analytical, dissolved oxygen measurements not provided)

GLP compliance:

yes

Specific details on test material used for the study:

- Substance type: quaternized ammonium compound
- Physical state: solid
- Storage condition of test material: ambient, isopropanol

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Method: A stock solution was prepared by mxing an appropriate amount of test solution with dilution water; test solutions were prepared by dilution of the stock solution in dilution water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): 4-24 hr old
- Method of breeding: adults (at least 3 weeks old) seperated from stock population by sieving
- Feeding during test: not fed during test

ACCLIMATION
- Acclimation conditions (same as test or not): acclimated in dilution water (same as test)
- Type and amount of food: algal suspension (Scenedesmus subspicata)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

268.5 mg/L

Test temperature:

18 - 22°C, for each single test constant within +/- 0.5 °C

pH:

7.8 +/- 2.0

Nominal and measured concentrations:

Nominal: 0.1, 0.32, 1.0, 3.2, 10, 32 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 ml beaker
- Renewal rate of test solution (frequency/flow rate): 24 h static test (no renewal)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared according to test guideline DIN 348 12 L 11
- Ca/Mg ratio: 4:1
- Intervals of water quality measurement: 0 and 24 hrs

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization at 24 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study
- Test concentrations: 0, 0.1, 1, 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

14.8 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 8.4 - 26.2

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 32 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mobility

Details on results:

- Effect concentrations exceeding solubility of substance in test medium: 32 mg/L (solubility <20 mg/L)

Reported statistics and error estimates:

LC50 or EC50, calculated according to Litchfield and Wilcoxon (1949).

Table: Immobilisation of daphnids

Conc. [mg/L]	Number of immobilized Daphnia	% of 20 Daphnia immobilized
control	0	0
0.10	0	0
0.32	0	0
1.00	0	0
3.20	2	10
10.00	8	40
32.00	14	70

Validity criteria fulfilled:

no

Remarks:

(dissolved oxygen concentrations not provided)

Conclusions:

The 24-hr acute toxicity of MDEA-Esterquat C16-18 and C18 unsatd. to Daphnia magna was investigated under static conditions in a study conducted according to OECD TG 202. The 24-h EC50 was determined to be 14.8 mg/L (nominal; 95% C.L.: 8.4 - 26.2 mg/L).

Executive summary:

The 24-hr acute toxicity of MDEA-Esterquat C16-18 and C18 unsatd. to Daphnia magna was investigated under static conditions in a study conducted according to OECD TG 202 (Part I). Daphnids were exposed to 0, 0.1, 0.32, 1.0, 3.2, 10 and 32 mg/L (nominal) for 24 hours. Immobilisation was observed at test termination. The 24-h EC50 was 14.8 mg/L with 95% CL of 8.4 - 26.2 mg/L (nominal each). No other effects were noted. The study period of 24 h was recommended before adoption of the OECD Guideline 202 in 2004. Therefore this study does not meet the time criterium (study duration 24 h instead of 48 h) of today standard methods.

This study, however, is classified as reliable with restrictions and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

Description of key information

Daphnia magna: 24-h EC50=14.8 mg/L (nominal; 95% C.L.: 8.4 - 26.2 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

14.8 mg/L

Additional information

The 24-hr acute toxicity of MDEA-Esterquat C16-18 and C18 unsatd. to Daphnia magna was investigated under static conditions in a study conducted according to OECD TG 202 (Part I). Daphnids were exposed to 0, 0.1, 0.32, 1.0, 3.2, 10 and 32 mg/L (nominal) for 24 hours. Immobilisation was observed at test termination. The 24-h EC50 was 14.8 mg/L with 95% CL of 8.4 - 26.2 mg/L (nominal each). No other effects were noted. The study period of 24 h was recommended before adoption of the OECD Guideline 202 in 2004. Therefore this study does not meet the time criterium (study duration 24 h instead of 48 h) of today standard methods.

Similar results were obtained with the closely related read-across substance MDIPA EsterquatC16-18 and C18 unsatd and MDIPA Esterquat C18 unsatd..:

The 48–hr-acute toxicity of MDIPA Esterquat C18 unsatd. to Daphnia magna was studied under semi-static conditions. Daphnids were exposed to control and test chemical at nominal concentrations of 0.10, 0.32, 1.0, 3.2, 10 mg/L, corresponding to measured (TWA) concentrations of 0.077, 0.27, 0.89, 2.8 and 8.6 mg/L in the presence of 4 mg/L humic acid for 48 h. Mortality/immobilisation and sublethal effects were observed daily. 

The 48-hour EC50 was >8.6 mg/L (TWA) based on immobilisation.  

The 48–hr-acute toxicity of MDIPA-Esterquat C16-18 and C18 unsatd. to Daphnia magna was studied under semi-static conditions. Daphnids were exposed to control and test chemical at analytically confirmed nominal concentrations of 0, 2.5, 4.5, 8.1, 14 and 25 mg/L in the presence of 4 mg/L (< 2mg/L DOC) humic acid for 48 h. Immobilisation was observed daily. 

The 48-hour EC50 was 6.7 mg/L (analytically confirmed nominal concentration) based on immobilisation.