1,3-Benzenedicarboxylic acid, 5,5'-[1,2-ethanediylbis[thio-1,3,4-thiadiazole-5,2-diyl-2,1-diazenediyl[5-amino-3-(1,1-dimethylethyl)-1H-pyrazole-4,1-diyl]]]bis-, potassium salt (1:4)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24.05.11 to 27.05.11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Report on substance meets the criteria for classification as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Test solutions prepared in dechlorinated water

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

tetrapotassium 5-{5-amino-4-[(1E)-2-(5-{[2-({5-[(1E)-2-[5-amino-3-tert-butyl-1-(3,5-dicarboxylatophenyl)-1H-pyrazol-4-yl]diazen-1-yl]-1,3,4-thiadiazol-2-yl}sulfanyl)ethyl]sulfanyl}-1,3,4-thiadiazol-2-yl)diazen-1-yl]-3-tert-butyl-1H-pyrazol-1-yl}benzene-1,3-dicarboxylate

EC Number:

700-616-6

Cas Number:

849608-59-9

Molecular formula:

C36H32K4N14O8S4

IUPAC Name:

tetrapotassium 5-{5-amino-4-[(1E)-2-(5-{[2-({5-[(1E)-2-[5-amino-3-tert-butyl-1-(3,5-dicarboxylatophenyl)-1H-pyrazol-4-yl]diazen-1-yl]-1,3,4-thiadiazol-2-yl}sulfanyl)ethyl]sulfanyl}-1,3,4-thiadiazol-2-yl)diazen-1-yl]-3-tert-butyl-1H-pyrazol-1-yl}benzene-1,3-dicarboxylate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 120 mg/L test substance (nominal)
- Sampling method; Samples taken at 0h from excess test solutions. Samples at 48h taken from single test replicate and analysed post centrifugation.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia sp.
- Source: Continuous laboratory culture at Brixham Environmental Laboratory
- Age at study initiation: <24h
- Method of breeding: diploid parthogenesis
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

50 mg/L CaCO3

Test temperature:

20.6-20.7 degrees centigrade

pH:

8.0-8.4

Dissolved oxygen:

8.2-8.4 mg/L (90-93% of air saturation value)

Salinity:

not applicable

Nominal and measured concentrations:

Test concentrations were dechlorinated medium control and nominal concentrations of 120 mg/L (nominal) test substance. The measured concentration in the nominal 120 mg/L was 100% of nominal at the start of the test and 20% of nominal at the end. On the basis of the analytical data the geometric mean measured concentration was used for calculating and reporting of the results.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250ml glass beakers
- Material, size, headspace, fill volume: 200ml of test solution providing a depth of approx 60mm
- Aeration: none
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water aerated overnight
- Conductivity: 201uS/cm

- Photoperiod: 16h light and 8h dark with 20 min. dusk/dawn transition

EFFECT PARAMETER MEASURED: Observations at 24h and 48h of whole body movement. Absence of movement in a 15s period is considered immobile

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 54 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

54 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

It is concluded that as none of the D.magna were observed as immobilised after 24 or 48h in the control or test concentration solutions and no other symptoms of toxicity were observed that the test substance has no toxic effect on Daphnia magna at 54 mg/L (based on geometric mean measured concentrations).